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Abstract:

On sudden presentation of a danger stimulus, the crab Chasmagnathus elicits an escape response that habituates promptly and far a long period. We have previously reported that administration of a cAMP-permeable analog (CPT-cAMP) along with a phosphodiesterase inhibitor (IBMX) improves long-term habituation (LTH). In present experiments we studied the effect of systemic administration of the protein kinase A (PKA) activator Sp-,5,6-DCl-cBIMPS and that of the PKA inhibitor Rp-8-Cl-cAMPS on LTH tested 24 h after a weak training protocol (5 trials of danger stimulus presentation) or a strong training protocol (15-30 trials), respectively. A 50 μl pre-training injection of 75 μM Sp-5,6-DCl-cBIMPS, and to a lesser degree of 25 μM, improved retention of the habituated response but not affect short-term habituation (STH). Like pre-training injection, post-training administration of Sp-5,6-DCl-cBIMPS proved to exert a facilitatory action on retention though with 75 μM dose only. Conversely, both pre- and post-training injection of 25 μM Rp-8-Cl-cAMPS impaired LTH without affecting STH. Thus, the PKA activator Sp-5,6-DCl-cBIMPS enables a weak training to produce LTH while the PKA inhibitor Rp-8-Cl-cAMPS impairs LTH when a strong training is given. Activation of crab PKA by Sp-5,6-DCl-cBIMPS and its inhibition by Rp-8-Cl-cAMPS were assessed using an in vitro PKA activity assay. These results provide independent evidences supporting the view that PKA plays a key role in long-term memory storage in this learning paradigm.

Registro:

Documento: Artículo
Título:Effects of activation and inhibition of cAMP-dependent protein kinase on long-term habituation in the crab Chasmagnathus
Autor:Romano, A.; Locatelli, F.; Delorenzi, A.; Pedreira, M.E.; Maldonado, H.
Filiación:Fac. de Ciencias Exactas y Naturales, Depto. de Cie. Biologicas, Pab 2., University of Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:cAMP analog; Crustacea; Habituation; Learning; Long-term memory; Protein kinase A; cyclic AMP; cyclic AMP dependent protein kinase; cyclic AMP derivative; phosphodiesterase inhibitor; protein kinase inhibitor; animal experiment; animal tissue; article; behavior; brain tissue; controlled study; crab; habituation; long term memory; male; nonhuman; priority journal
Año:1996
Volumen:735
Número:1
Página de inicio:131
Página de fin:140
DOI: http://dx.doi.org/10.1016/0006-8993(96)00584-7
Título revista:Brain Research
Título revista abreviado:BRAIN RES.
ISSN:00068993
CODEN:BRREA
CAS:cyclic AMP, 60-92-4; cyclic AMP dependent protein kinase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v735_n1_p131_Romano

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Citas:

---------- APA ----------
Romano, A., Locatelli, F., Delorenzi, A., Pedreira, M.E. & Maldonado, H. (1996) . Effects of activation and inhibition of cAMP-dependent protein kinase on long-term habituation in the crab Chasmagnathus. Brain Research, 735(1), 131-140.
http://dx.doi.org/10.1016/0006-8993(96)00584-7
---------- CHICAGO ----------
Romano, A., Locatelli, F., Delorenzi, A., Pedreira, M.E., Maldonado, H. "Effects of activation and inhibition of cAMP-dependent protein kinase on long-term habituation in the crab Chasmagnathus" . Brain Research 735, no. 1 (1996) : 131-140.
http://dx.doi.org/10.1016/0006-8993(96)00584-7
---------- MLA ----------
Romano, A., Locatelli, F., Delorenzi, A., Pedreira, M.E., Maldonado, H. "Effects of activation and inhibition of cAMP-dependent protein kinase on long-term habituation in the crab Chasmagnathus" . Brain Research, vol. 735, no. 1, 1996, pp. 131-140.
http://dx.doi.org/10.1016/0006-8993(96)00584-7
---------- VANCOUVER ----------
Romano, A., Locatelli, F., Delorenzi, A., Pedreira, M.E., Maldonado, H. Effects of activation and inhibition of cAMP-dependent protein kinase on long-term habituation in the crab Chasmagnathus. BRAIN RES. 1996;735(1):131-140.
http://dx.doi.org/10.1016/0006-8993(96)00584-7