Abstract:
The effect of glucocorticoids on (Na + K)ATPase mRNA synthesis was studied in 19 brain areas of adrenalectomized (ADX) rats untreated or receiving dexamethasone (DEX). For in situ hybridization, we employed a [35S]oligonucleotide probe for the α3-subunit isoform, and a [3H]cDNA coding for the β1-subunit of the enzyme. Mean levels of grain density for the α3 subunit mRNA of DEX-treated rats were significantly higher by a 't' test in medial septum, amygdala lateralis (AL) and medialis (AME), gyrus dentatus, CA4 hippocampal area, substantia nigra and periventricular gray, compared to untreated rats. For the β1-subunit, mean levels after DEX were significantly higher in AL and lateral preoptic area. In addition, the Kolmogorov-Smirnov test applied to frequency histograms of neuronal densities indicated a coordinate increase in α3 and β1-subunit mRNA expression for the CA2 subfield and preoptic area medialis (POA MED). We conclude that (1) glucocorticoids are positive modulators of (Na + K)ATPase mRNA; (2) analysis of frequency histograms suggests that glucocorticoids promote in a few regions (AL, POA MED, CA2 subfield) a coordinate increase in the biosynthesis of the α3 and β1-subunit mRNA. In 11 other areas stimulation occurs for one subunit mRNA only, whereas 5 areas were insensitive to glucocorticoid effects on this enzyme. © 1994.
Registro:
Documento: |
Artículo
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Título: | Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization |
Autor: | Grillo, C.; Piroli, G.; González, S.L.; Angulo, J.; McEwen, B.S.; De Nicola, A.F. |
Filiación: | Laboratory of Neuroendocrine Biochemistry, Instituto de Biología y Medicina Experimental-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina The Rockefeller University, New York, NY, United States
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Palabras clave: | (Na + K)ATPase; Adrenalectomy; Dexamethasone; In situ hybridization; adenosine triphosphatase (potassium sodium); dexamethasone; glucocorticoid; adrenalectomy; animal experiment; animal tissue; article; brain region; controlled study; enzyme activity; in situ hybridization; male; nonhuman; oral drug administration; priority journal; rat; rna synthesis; Adrenal Glands; Adrenalectomy; Animal; Base Sequence; Brain; Dexamethasone; Genetic Code; In Situ Hybridization; Male; Molecular Sequence Data; Na(+)-K(+)-Exchanging ATPase; Peptide Fragments; Rats; Rats, Sprague-Dawley; RNA, Messenger |
Año: | 1994
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Volumen: | 657
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Número: | 1-2
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Página de inicio: | 83
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Página de fin: | 91
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DOI: |
http://dx.doi.org/10.1016/0006-8993(94)90956-3 |
Título revista: | Brain Research
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Título revista abreviado: | Brain Res.
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ISSN: | 00068993
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CODEN: | BRREA
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CAS: | dexamethasone, 50-02-2; Dexamethasone, 50-02-2; Na(+)-K(+)-Exchanging ATPase, EC 3.6.1.37; Peptide Fragments; RNA, Messenger
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v657_n1-2_p83_Grillo |
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Citas:
---------- APA ----------
Grillo, C., Piroli, G., González, S.L., Angulo, J., McEwen, B.S. & De Nicola, A.F.
(1994)
. Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization. Brain Research, 657(1-2), 83-91.
http://dx.doi.org/10.1016/0006-8993(94)90956-3---------- CHICAGO ----------
Grillo, C., Piroli, G., González, S.L., Angulo, J., McEwen, B.S., De Nicola, A.F.
"Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization"
. Brain Research 657, no. 1-2
(1994) : 83-91.
http://dx.doi.org/10.1016/0006-8993(94)90956-3---------- MLA ----------
Grillo, C., Piroli, G., González, S.L., Angulo, J., McEwen, B.S., De Nicola, A.F.
"Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization"
. Brain Research, vol. 657, no. 1-2, 1994, pp. 83-91.
http://dx.doi.org/10.1016/0006-8993(94)90956-3---------- VANCOUVER ----------
Grillo, C., Piroli, G., González, S.L., Angulo, J., McEwen, B.S., De Nicola, A.F. Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization. Brain Res. 1994;657(1-2):83-91.
http://dx.doi.org/10.1016/0006-8993(94)90956-3