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González Deniselle, M.C.; Garay, L.; López-Costa, J.J.; González, S.; Mougel, A.; Guennoun, R.; Schumacher, M.; De Nicola, A.F. "Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease" (2004) Brain Research. 1014(1-2):71-79
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Abstract:

Previous work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS). Since nitric oxide is a highly reactive species, it may play a role in neuropathology of Wr mice. We now studied if PROG neuroprotection involved changes of NOS activity in motoneurons and astrocytes, determined by the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHD) histochemical reaction. Two and four-month-old Wr mice at the progressive and stabilization stages of the disease, respectively, and their age-matched controls were left untreated or received a single 20-mg PROG pellet for 18 days. PROG reduced the high number of NADPHD-active motoneurons and white matter astrocytes in 2-month-old Wr mice but was unable to change the low number of NADPHD-active motoneurons in 4-month-old Wr mice or astrocytes in this age group. A large number of motoneurons in 2-month-old Wr mice showed a vacuolated phenotype, which was significantly reverted by PROG treatment. In summary, PROG treatment during the early symptomatic stage of the disease caused a significant reduction of NADPHD-active motoneurons and astrocytes and also reduced vacuolated degenerating cells, suggesting that blockade of NO synthesis and oxidative damage may contribute to steroid neuroprotection. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease
Autor:González Deniselle, M.C.; Garay, L.; López-Costa, J.J.; González, S.; Mougel, A.; Guennoun, R.; Schumacher, M.; De Nicola, A.F.
Filiación:Lab. of Neuroendocrine Biochemistry, Inst. de Biol. Y Med. Experimental, University of Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
Inst. Biol. Cel. Y Neurociencias P., Facultad de Medicina, University of Buenos Aires, Argentina
Inst. Univ. de Ciencias de la Salud, Fundación Barceló, Buenos Aires, Argentina
INSERM U488, Hôpital de Bicêtre, Paris, France
Palabras clave:Endocrine and autonomic regulation; Motoneuron disease; Neuroendocrine regulation: other; Neuroprotection; Nitric oxide synthase; Progesterone; Wobbler mouse; 3alpha hydroxy 5alpha pregnan 20 one; 5alpha pregnane 3,20 dione; methylprednisolone; nitric oxide synthase; progesterone; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; animal model; animal tissue; article; astrocyte; cell vacuole; controlled study; disease course; drug blood level; drug effect; drug potency; enzyme activity; female; histochemistry; male; motoneuron; motor neuron disease; mouse; neuroprotection; nonhuman; phenotype; priority journal; white matter; Animals; Astrocytes; Cell Count; Down-Regulation; Female; Male; Mice; Mice, Neurologic Mutants; Motor Neuron Disease; Motor Neurons; NADPH Dehydrogenase; Nitric Oxide Synthase; Progesterone
Año:2004
Volumen:1014
Número:1-2
Página de inicio:71
Página de fin:79
DOI: http://dx.doi.org/10.1016/j.brainres.2004.04.004
Título revista:Brain Research
Título revista abreviado:Brain Res.
ISSN:00068993
CODEN:BRREA
CAS:3alpha hydroxy 5alpha pregnan 20 one, 516-54-1; 5alpha pregnane 3,20 dione, 566-65-4; methylprednisolone, 6923-42-8, 83-43-2; nitric oxide synthase, 125978-95-2; progesterone, 57-83-0; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase, 9001-68-7; NADPH Dehydrogenase, EC 1.6.99.1; Nitric Oxide Synthase, EC 1.14.13.39; Progesterone, 57-83-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle

Referencias:

  • Abe, K., Morita, S., Kikuchi, T., Itoyama, Y., Protective effect of a novel free-radical scavenger, OPC-14117, on Wobbler mouse motor neuron disease (1997) J. Neurosci. Res., 48, pp. 63-70
  • Azcoitia, I., Leonelli, E., Magnaghi, V., Veiga, S., Garcia-Segura, L.M., Melcangi, R.C., Progesterone and its derivatives dihydroprogesterone and tetrahydroprogesterone reduce myelin fiber morphological abnormalities and myelin fiber loss in the sciatic nerve of aged rats (2003) Neurobiol. Aging, 24, pp. 853-860
  • Beal, M.F., Mitochondrial dysfunction in neurodegenerative diseases (1998) Biochem. Biophys. Acta, 1366, pp. 217-233
  • Boillée, S., Peschanski, M., Junier, M.-P., The Wobbler mouse: A neurodegeneration jigsaw puzzle (2003) Mol. Neurobiol., 28, pp. 65-106
  • Clarke, P.G.H., Developmental cell death: Morphological diversity and multiple mechanisms (1990) Anat. Embryol., 181, pp. 195-213
  • Clowry, G.J., McHanwell, S., Expression of nitric oxide synthase by motor neurons in the spinal cord of the mutant mouse Wobbler (1996) Neurosci. Lett., 215, pp. 177-180
  • Corvino, V., Businaro, R., Geloso, M.C., Bigini, P., Caballo, V., Pompili, E., Mennini, T., Michetti, F., S100B protein and 4-hydroxynonenal in the spinal cord of Wobbler mice (2003) Neurochem. Res., 28, pp. 341-345
  • Dave, K.R., Bradley, W.G., Pérez Pinzón, M.A., Early mitochondrial dysfunction occurs in motor cortex and spinal cord at the onset of disease in the Wobbler mouse (2003) Exp. Neurol., 182, pp. 412-420
  • Desarnaud, F., Do Thi, A.M., Brown, G., Lemke, G., Suter, U., Baulieu, E.E., Schumacher, M., Progesterone stimulates the activity of the promoters of peripheral myelin protein-22 and protein zero genes in Schwann cells (1998) J. Neurochem., 71, pp. 1765-1768
  • González Deniselle, M.C., Grillo, C., González, S., Roig, P., De Nicola, A.F., Evidence for down-regulation of GAP-43 mRNA in Wobbler mouse spinal motoneurons by corticosterone and a 21-aminosteroid (1999) Brain Res., 841, pp. 78-84
  • González Deniselle, M.C., González, S., Lima, A., Wilkin, G., De Nicola, A.F., The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPHDiaphorase in the spinal cord of Wobbler mouse, a model for Amyotrophic Lateral Sclerosis (1999) Neurochem. Res., 24, pp. 1-8
  • González Deniselle, M.C., González, S., De Nicola, A.F., Cellular basis of steroid neuroprotection in the Wobbler mouse, a model for motoneuron disease (2001) Cell. Mol. Neurobiol., 21, pp. 237-254
  • González Deniselle, M.C., López Costa, J.J., Pecci Saavedra, J., Pietranera, L., González, S., Garay, L., Guennoun, R., De Nicola, A.F., Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease (2002) Neurobiol. Dis., 11, pp. 457-468
  • González Deniselle, M.C., López Costa, J.J., González, S., Labombarda, F., Garay, L., Guennoun, R., Schumacher, M., De Nicola, A.F., Basis of progesterone protection in spinal cord neurodegeneration (2003) J. Steroid Biochem. Mol. Biol., 83, pp. 199-209
  • Guang-Ping, X., Dave, K.R., Moraes, C.T., Busto, R., Sick, T.J., Bradley, W.G., Pérez-Pinzón, M.A., Dysfunctional mitochondrial respiration in the Wobbler mouse brain (2001) Neurosci. Lett., 300, pp. 141-144
  • Gurney, M.E., What transgenic mice tell us about neurodegenerative disease (2000) BioEssays, 22, pp. 297-304
  • Hall, E.D., Neuroprotective actions of glucocorticoid and nonglucocorticoid steroids in acute neuronal injury (1993) Cell. Mol. Neurobiol., 13, pp. 415-432
  • Hall, E.D., Braughler, J.M., Effects of intravenous methylprednisolone on spinal cord lipid peroxidation and (Na, K)-ATPase activity. Dose response analysis during 1st hour after contusion injury in the cat (1982) J. Neurosurg., 49, pp. 563-568
  • Henderson, J.T., Javaheri, M., Kopko, S., Roder, J.C., Reduction of lower motor neuron degeneration in wobbler mice by n-acetyl-L-cysteine (2000) J. Neurosci., 16, pp. 7574-7582
  • Ikeda, K., Iwasaki, Y., Kinoshita, M., Neuronal nitric oxide synthase inhibitor, 7-nitroindazole, delays motor dysfunction and spinal motoneuron degeneration in the Wobbler mouse (1988) J. Neurol. Sci., 160, pp. 9-15
  • Koenig, H.L., Schumacher, M., Ferzaz, B., Do Thi, A.N., Ressouches, A., Guennoun, R., Jung-Testas, I., Baulieu, E.E., Progesterone synthesis and myelin formation by Schwann cells (1995) Science, 268, pp. 1500-1502
  • Kong, J., Zu, Z., Massive mitochondrial degeneration in motor neurons triggers the onset of amyotrophic lateral sclerosis in mice expressing a mutant SOD1 (1998) J. Neurosci., 18, pp. 3241-3250
  • Labombarda, F., González, S., Roig, P., Lima, A., Guennoun, R., Schumacher, M., De Nicola, A.F., Modulation of NADPHDiaphorase and glial fibrillary acidic protein by progesterone in astrocytes from normal and injured spinal cord (2000) J. Steroid Biochem. Mol. Biol., 73, pp. 159-169
  • Labombarda, F., González, S., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F., Cellular basis for progesterone neuroprotection in the injured spinal cord (2002) J. Neurotrauma, 19, pp. 343-355
  • Labombarda, F., González, S., González Deniselle, M.C., Vinson, G.P., Schumacher, M., De Nicola, A.F., Guennoun, R., Effects of injury and progesterone treatment on progesterone receptor and progesterone binding protein 25 Dx in the rat spinal cord (2003) J. Neurochem., 87, pp. 902-913
  • Lehmann, A., (1974) Atlas Stereotaxique du Cerveau de la Souris, , Paris: Editions du Centre National de la Recherche Scientifique
  • Melcangi, R.C., Magnaghi, V., Martini, L., Aging in peripheral nerves: Regulation of myelin protein genes by steroid hormones (2000) Prog. Neurobiol., 60, pp. 291-308
  • Mitsumoto, H., Bradley, W.G., Murine motor neuron disease (the Wobbler mouse): Degeneration and regeneration of the lower motor neuron (1982) Brain Res., 105, pp. 811-834
  • Phul, R.K., Shaw, P.J., Ince, P.G., Smith, M.E., Expression of nitric oxide synthase isoforms in spinal cord in amyotrophic lateral sclerosis (2000) Amyotroph. Lateral Scler. Other Mot. Neuron Disord., 4, pp. 259-267
  • Radi, R., Beckman, J.S., Bush, K.M., Freeman, B.A., Peroxynitrite-induced membrane lipid peroxidation: The cytotoxic potential of superoxide and nitric oxide (1991) Arch. Biochem. Biophys., 288, pp. 481-487
  • Roof, R., Hall, E.D., Gender differences in acute CNS trauma and stroke: Neuroprotective effects and progesterone (2000) J. Neurotrauma, 17, pp. 367-388
  • Roof, R., Duvdevani, R., Braswell, L., Stein, D.G., Progesterone facilitates cognitive recovery and reduces secondary neuronal loss caused by cortical contusion injury in male rats (1994) Exp. Neurol., 129, pp. 64-69
  • Roof, R., Hoffman, S., Stein, D.G., Progesterone protects against lipid peroxidation following traumatic brain injury in rats (1997) Mol. Chem. Neuropathol., 31, pp. 1-11
  • Rupprecht, R., Reul, J.M., Trapp, T., Van Steensel, B., Wetzel, C., Damm, K., Zieglgansberger, W., Holsboer, F., Progesterone receptor-mediated effects of neuroactive steroids (1993) Neuron, 11, pp. 523-530
  • Saravia, F., González, S., Roig, P., Alves, V., Homo-Delarche, F., De Nicola, A.F., Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of type I diabetes, the nonobese diabetic (NOD) mouse (2001) Cell. Mol. Neurobiol., 21, pp. 15-27
  • Sasaki, S., Warita, H., Abe, K., Iwata, M., Neuronal nitric oxide synthase (nNos) immunoreactivity in the spinal cord of transgenic mice with G93A mutant SOD1 gene (2002) Acta Neuropathol., 103, pp. 421-427
  • Schumacher, M., Akwa, I., Guennoun, R., Robert, F., Labombarda, F., Desarnaud, F., Robel, P., Baulieu, E.E., Steroid synthesis and metabolism in the nervous system: Trophic and protective effects (2000) J. Neurocytol., 29, pp. 307-326
  • Stein, D.G., Fulop, Z.L., Progesterone and recovery after traumatic brain injury: An overview (1998) Neuroscientist, 4, pp. 435-442
  • Stewart, V.C., Heslegrave, A.J., Brown, G.C., Clark, J.B., Heales, J.R., Nitric-oxide dependent damage to neuronal mitochondria involves the NMDA receptor (2002) Eur. J. Neurosci., 15, pp. 458-464
  • Thomas, A., Nockels, R., Pan, H., Shaffrey, C., Chopp, M., Progesterone is neuroprotective after acute experimental spinal cord trauma in rats (1999) Spine, 24, pp. 2134-2138
  • Tsuzaka, K., Ishiyama, T., Pioro, E.P., Mitsumoto, H., Role of brain-derived neurotrophic factor in Wobbler mouse motor neuron disease (2001) Muscle Nerve, 24, pp. 474-480
  • Vincent, S.R., Kimura, H., Histochemical mapping of nitric oxide synthase in the rat brain (1992) Neuroscience, 46, pp. 755-784
  • Yung, K.K.L., Tang, F., Vacca-Galloway, L.L., Changes of neuropeptides in spinal cord and brain stem of Wobbler mouse at different stages of motoneuron disease (1992) Neuroscience, 50, pp. 209-222

Citas:

---------- APA ----------
González Deniselle, M.C., Garay, L., López-Costa, J.J., González, S., Mougel, A., Guennoun, R., Schumacher, M.,..., De Nicola, A.F. (2004) . Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease. Brain Research, 1014(1-2), 71-79.
http://dx.doi.org/10.1016/j.brainres.2004.04.004
---------- CHICAGO ----------
González Deniselle, M.C., Garay, L., López-Costa, J.J., González, S., Mougel, A., Guennoun, R., et al. "Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease" . Brain Research 1014, no. 1-2 (2004) : 71-79.
http://dx.doi.org/10.1016/j.brainres.2004.04.004
---------- MLA ----------
González Deniselle, M.C., Garay, L., López-Costa, J.J., González, S., Mougel, A., Guennoun, R., et al. "Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease" . Brain Research, vol. 1014, no. 1-2, 2004, pp. 71-79.
http://dx.doi.org/10.1016/j.brainres.2004.04.004
---------- VANCOUVER ----------
González Deniselle, M.C., Garay, L., López-Costa, J.J., González, S., Mougel, A., Guennoun, R., et al. Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease. Brain Res. 2004;1014(1-2):71-79.
http://dx.doi.org/10.1016/j.brainres.2004.04.004