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Abstract:

Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.

Registro:

Documento: Artículo
Título:Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval
Autor:Fraiman, D.; Pando, B.; Dargan, S.; Parker, I.; Dawson, S.P.
Filiación:Departamento de Física, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Department of Neurobiology and Behavior, University of California, Irvine, CA, United States
Depto. de Física, Facultad de Ciencias Exactas Y Naturales, Pabellón I, (1428), Buenos Aires, Argentina
Palabras clave:1,4,5 triphosphate; calcium; phosphate; unclassified drug; animal cell; article; controlled study; dynamics; endoplasmic reticulum; fluorescence; Kolmogorov Smirnov test; muscle cell; nonhuman; oocyte; Xenopus laevis; Neptunia
Año:2006
Volumen:90
Número:11
Página de inicio:3897
Página de fin:3907
DOI: http://dx.doi.org/10.1529/biophysj.105.075911
Título revista:Biophysical Journal
Título revista abreviado:Biophys. J.
ISSN:00063495
CODEN:BIOJA
CAS:calcium, 7440-70-2, 14092-94-5; phosphate, 14066-19-4, 14265-44-2
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00063495_v90_n11_p3897_Fraiman.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v90_n11_p3897_Fraiman

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Citas:

---------- APA ----------
Fraiman, D., Pando, B., Dargan, S., Parker, I. & Dawson, S.P. (2006) . Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval. Biophysical Journal, 90(11), 3897-3907.
http://dx.doi.org/10.1529/biophysj.105.075911
---------- CHICAGO ----------
Fraiman, D., Pando, B., Dargan, S., Parker, I., Dawson, S.P. "Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval" . Biophysical Journal 90, no. 11 (2006) : 3897-3907.
http://dx.doi.org/10.1529/biophysj.105.075911
---------- MLA ----------
Fraiman, D., Pando, B., Dargan, S., Parker, I., Dawson, S.P. "Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval" . Biophysical Journal, vol. 90, no. 11, 2006, pp. 3897-3907.
http://dx.doi.org/10.1529/biophysj.105.075911
---------- VANCOUVER ----------
Fraiman, D., Pando, B., Dargan, S., Parker, I., Dawson, S.P. Analysis of puff dynamics in oocytes: Interdependence of puff amplitude and interpuff interval. Biophys. J. 2006;90(11):3897-3907.
http://dx.doi.org/10.1529/biophysj.105.075911