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Abstract:

CRISP2, originally known as Tpx-1, is a cysteine-rich secretory protein specifically expressed in male haploid germ cells. Although likely to be involved in gamete interaction, evidence for a functional role of CRISP2 in fertilization still remains poor. In the present study, we used a mouse model to examine the subcellular localization of CRISP2 in sperm and its involvement in the different stages of fertilization. Results from indirect immunofluorescence and protein extraction experiments indi-cated that mouse CRISP2 is an intraacrosomal component that remains associated with sperm after capacitation and the acrosome reaction (AR). In vitro fertilization assays using zona pellucida-intact mouse eggs showed that an antibody against the protein significantly decreased the percentage of penetrated eggs, with a coincident accumulation of perivitelline sperm. The failure to inhibit zona pellucida penetration excludes a detrimental effect of the antibody on sperm motility or the AR, supporting a specific participation of CRSSP2 at the sperm-egg fusion step. In agreement with this evidence, recombinant mouse CRISP2 (recCRISP2) specifically bound to the fusogenic area of mouse eggs, as previously reported for rat CRISP1, an epididymal protein involved in gamete fusion. In vitro competition investigations showed that incubation of mouse zona-free eggs with a fixed concentration of recCRISP2 and increasing amounts of rat CRISP1 reduced the binding of recCRISP2 to the egg, suggesting that the proteins interact with common complementary sites on the egg surface. Our findings indicate that testicular CRISP2, as observed for epididymal CRISP1, is involved in sperm-egg fusion through its binding to complementary sites on the egg surface, supporting the idea of functional cooperation between homologous molecules to ensure the success of fertilization. © 2007 by the Society for the Study of Reproduction, Inc.

Registro:

Documento: Artículo
Título:Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion
Autor:Busso, D.; Goldweic, N.M.; Hayashi, M.; Kasahara, M.; Cuasnicú, P.S.
Filiación:Instituto de Biología y Medicina Experimental, 1428 Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 6513492 Santiago, Chile
Department of Obstetrics, Obihiro Kousei Hospital, 080-0016 Obihiro, Japan
Department of Pathology, Hokkaido University, Graduate School of Medicine, 060-8638 Sapporo, Japan
Palabras clave:Fertilization; Ovum; Sperm; Testis; cell protein; isoprotein; protein antibody; protein CRISP1; protein CRISP2; recombinant protein; secretory protein; unclassified drug; acrosome reaction; animal cell; animal tissue; article; cell fusion; cell surface; cellular distribution; controlled study; epididymis; female; fertilization; fertilization in vitro; human; immunofluorescence; male; mouse; nonhuman; priority journal; protein analysis; protein binding; protein function; protein localization; spermatozoon capacitation; spermatozoon motility; spermatozoon penetration; testis; vitelline membrane; zona pellucida; Animals; Female; Glycoproteins; Male; Mice; Mice, Inbred Strains; Sperm-Ovum Interactions; Spermatozoa; Testis; Rattus
Año:2007
Volumen:76
Número:4
Página de inicio:701
Página de fin:708
DOI: http://dx.doi.org/10.1095/biolreprod.106.056770
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:Crisp2 protein, mouse; Glycoproteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v76_n4_p701_Busso

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Citas:

---------- APA ----------
Busso, D., Goldweic, N.M., Hayashi, M., Kasahara, M. & Cuasnicú, P.S. (2007) . Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biology of Reproduction, 76(4), 701-708.
http://dx.doi.org/10.1095/biolreprod.106.056770
---------- CHICAGO ----------
Busso, D., Goldweic, N.M., Hayashi, M., Kasahara, M., Cuasnicú, P.S. "Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion" . Biology of Reproduction 76, no. 4 (2007) : 701-708.
http://dx.doi.org/10.1095/biolreprod.106.056770
---------- MLA ----------
Busso, D., Goldweic, N.M., Hayashi, M., Kasahara, M., Cuasnicú, P.S. "Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion" . Biology of Reproduction, vol. 76, no. 4, 2007, pp. 701-708.
http://dx.doi.org/10.1095/biolreprod.106.056770
---------- VANCOUVER ----------
Busso, D., Goldweic, N.M., Hayashi, M., Kasahara, M., Cuasnicú, P.S. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod. 2007;76(4):701-708.
http://dx.doi.org/10.1095/biolreprod.106.056770