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Abstract:

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic and endocrine-disruptive actions of aromatic compounds in the ovary. Paradoxically, this receptor has been shown to play important roles in normal female reproductive function as well. Although knowledge of AHR expression regulation in the ovary is of crucial significance to understand the receptor biology and its function in reproductive physiology, there are only limited data in this area. The purpose of the present study was to establish the possible regulation that AHR might undergo in ovarian cells. Here we show that the hormones FSH and estradiol are able to reduce AHR protein and transcript levels in granulosa cells in a way that parallels the changes observed in ovarian tissue across the rat estrous cycle. These findings suggest that estradiol and FSH would be cycle-associated endogenous modulators of AHR expression. In addition, we show that in granulosa cells the receptor is rapidly downregulated via proteasomal degradation following treatment with AHR ligands. However, prolonged treatment with an agonist caused an increase in Ahr mRNA levels. These actions would constitute a regulatory mechanism that both attenuates AHR signal rapidly and replenishes the cellular receptor pool in the long term. In conclusion, our results indicate that AHR expression is regulated by classical hormones and by its own ligands in granulosa cells. © 2006 by the Society for the Study of Reproduction, Inc.

Registro:

Documento: Artículo
Título:Regulation of aryl hydrocarbon receptor expression in rat granulosa cells
Autor:Bussmann, U.A.; Barañao, J.L.
Filiación:Instituto de Biología y Medicina Experimental-CONICET, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Estradiol; Follicle-stimulating hormone; Granulosa cells; Ovary; Toxicology; aromatic compound; aromatic hydrocarbon receptor; cell receptor; endocrine disruptor; estradiol; follitropin; hormone; ligand; messenger RNA; transcription factor; animal cell; article; controlled study; down regulation; estrus cycle; female; female genital system; granulosa cell; hormonal regulation; nonhuman; ovary; ovary cell culture; physiology; priority journal; protein expression; protein function; rat; receptor upregulation; reproduction; signal transduction; Animals; beta-Naphthoflavone; Blotting, Western; Cell Line; Cytochrome P-450 CYP1A1; Down-Regulation; Enzyme Inhibitors; Estradiol; Female; Follicle Stimulating Hormone; Gene Expression Regulation; Granulosa Cells; Ovary; Proteasome Endopeptidase Complex; Rats; Rats, Sprague-Dawley; Receptors, Aryl Hydrocarbon; Reproduction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger
Año:2006
Volumen:75
Número:3
Página de inicio:360
Página de fin:369
DOI: http://dx.doi.org/10.1095/biolreprod.106.053017
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:estradiol, 50-28-2; follitropin, 9002-68-0; beta-Naphthoflavone, 6051-87-2; Cytochrome P-450 CYP1A1, EC 1.14.14.1; Enzyme Inhibitors; Estradiol, 50-28-2; Follicle Stimulating Hormone, 9002-68-0; Proteasome Endopeptidase Complex, EC 3.4.25.1; Receptors, Aryl Hydrocarbon; RNA, Messenger
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00063363_v75_n3_p360_Bussmann.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v75_n3_p360_Bussmann

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Citas:

---------- APA ----------
Bussmann, U.A. & Barañao, J.L. (2006) . Regulation of aryl hydrocarbon receptor expression in rat granulosa cells. Biology of Reproduction, 75(3), 360-369.
http://dx.doi.org/10.1095/biolreprod.106.053017
---------- CHICAGO ----------
Bussmann, U.A., Barañao, J.L. "Regulation of aryl hydrocarbon receptor expression in rat granulosa cells" . Biology of Reproduction 75, no. 3 (2006) : 360-369.
http://dx.doi.org/10.1095/biolreprod.106.053017
---------- MLA ----------
Bussmann, U.A., Barañao, J.L. "Regulation of aryl hydrocarbon receptor expression in rat granulosa cells" . Biology of Reproduction, vol. 75, no. 3, 2006, pp. 360-369.
http://dx.doi.org/10.1095/biolreprod.106.053017
---------- VANCOUVER ----------
Bussmann, U.A., Barañao, J.L. Regulation of aryl hydrocarbon receptor expression in rat granulosa cells. Biol. Reprod. 2006;75(3):360-369.
http://dx.doi.org/10.1095/biolreprod.106.053017