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Abstract:

Analogs of GnRH, including agonists (GnRH-a) and antagonists (GnRH-ant), have been widely used to inhibit gonadotropin pituitary release. Aside from the effect of GnRH analogs on the pituitary-gonadal axis, studies have shown that GnRH has extrapituitary effects, particularly on rat and human ovaries. In the present study, we evaluated the direct in vivo effects of the GnRH-a, leuprolide acetate (LA), or the GnRH-ant, Antide (Ant), either singly or together, on ovarian follicular development in prepubertal eCG-treated rats. LA significantly decreased ovarian weight, whereas Ant increased ovarian weight compared with controls; however, coinjection of both compounds had no effect. In addition, LA increased the number of preantral follicles (PFs) and atretic follicles, and decreased the number of early antral follicles (EAFs) and preovulatory follicles (POFs). Coinjection of Ant interfered with this LA effect. Ant alone increased the number of POFs compared with that of controls. Analysis of apoptosis has shown that LA increases the percentage of apoptotic cells in PFs, EAFs, and POFs; however, Ant prevented this effect. In addition, Ant alone decreased the percentage of apoptotic cells in EAFs and POFs. Data have shown that Ant per se inhibited BAX translocation from cytosol to mitochondria and retained cytochrome C in the mitochondria, whereas LA induced cytochrome C release. We conclude that Ant inhibits apoptosis in preovulatory follicles through a decrease of BAX translocation to mitochondria, suggesting that GnRH may act as a physiological intraovarian modulator factor that is able to interfere with follicular development through an increase in apoptotic events mediated by an imbalance among the BCL-2 family members.

Registro:

Documento: Artículo
Título:Gonadotropin-releasing hormone antagonist antide inhibits apoptosis of preovulatory follicle cells in rat ovary
Autor:Parborell, F.; Irusta, G.; Vitale, A.; Gonzalez, O.; Pecci, A.; Tesone, M.
Filiación:Inst. de Biol. Y Med. Experimental, CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. de Quím. Biol., Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. Fisiol., Biol. Molec. Y Cel., Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Inst. de Biol. Y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Apoptosis; Follicle; Gonadotropin-releasing hormone; Ovary; 4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid; cytochrome c; diethylstilbestrol; gonadorelin agonist; gonadorelin antagonist; iturelix; leuprorelin; protein Bax; protein bcl 2; agonist; animal cell; animal experiment; apoptosis; article; cell count; comparative study; controlled study; cytosol; drug effect; drug inhibition; female; gonadotropin release; hormone inhibition; mitochondrial membrane; nonhuman; organ weight; ovary follicle; ovary follicle atresia; ovary follicle maturation; ovary weight; prepuberty; priority journal; protein family; protein synthesis inhibition; rat; species comparison; Animals; Apoptosis; bcl-2-Associated X Protein; Cytochromes c; Estrous Cycle; Female; Gonadotropin-Releasing Hormone; Hormone Antagonists; Leuprolide; Oligopeptides; Ovarian Follicle; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley; Animalia; Formicidae
Año:2005
Volumen:72
Número:3
Página de inicio:659
Página de fin:666
DOI: http://dx.doi.org/10.1095/biolreprod.104.034454
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid, 7365-45-9; cytochrome c, 9007-43-6, 9064-84-0; diethylstilbestrol, 30498-85-2, 56-53-1; iturelix, 112568-12-4; leuprorelin, 53714-56-0, 74381-53-6; protein bcl 2, 219306-68-0; Bax protein, rat; bcl-2-Associated X Protein; Cytochromes c, 9007-43-6; Gonadotropin-Releasing Hormone, 33515-09-2; Hormone Antagonists; iturelix, 112568-12-4; Leuprolide, 53714-56-0; Oligopeptides; Proto-Oncogene Proteins c-bcl-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v72_n3_p659_Parborell

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Citas:

---------- APA ----------
Parborell, F., Irusta, G., Vitale, A., Gonzalez, O., Pecci, A. & Tesone, M. (2005) . Gonadotropin-releasing hormone antagonist antide inhibits apoptosis of preovulatory follicle cells in rat ovary. Biology of Reproduction, 72(3), 659-666.
http://dx.doi.org/10.1095/biolreprod.104.034454
---------- CHICAGO ----------
Parborell, F., Irusta, G., Vitale, A., Gonzalez, O., Pecci, A., Tesone, M. "Gonadotropin-releasing hormone antagonist antide inhibits apoptosis of preovulatory follicle cells in rat ovary" . Biology of Reproduction 72, no. 3 (2005) : 659-666.
http://dx.doi.org/10.1095/biolreprod.104.034454
---------- MLA ----------
Parborell, F., Irusta, G., Vitale, A., Gonzalez, O., Pecci, A., Tesone, M. "Gonadotropin-releasing hormone antagonist antide inhibits apoptosis of preovulatory follicle cells in rat ovary" . Biology of Reproduction, vol. 72, no. 3, 2005, pp. 659-666.
http://dx.doi.org/10.1095/biolreprod.104.034454
---------- VANCOUVER ----------
Parborell, F., Irusta, G., Vitale, A., Gonzalez, O., Pecci, A., Tesone, M. Gonadotropin-releasing hormone antagonist antide inhibits apoptosis of preovulatory follicle cells in rat ovary. Biol. Reprod. 2005;72(3):659-666.
http://dx.doi.org/10.1095/biolreprod.104.034454