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Abstract:

The participation of type I GnRH receptor (GnRH-R) on GnRH-II-induced gonadotropin secretion in rat pituitary cells was investigated. Furthermore, we extended the study of GnRH-II action to ovarian cells. The GnRH-II was able to mobilize inositol triphosphate (IP3) and to induce LH and FSH release in a dose-dependent manner in pituitary cells and in a GnRH-I-like manner. The GnRH-analog 135-18 (agonist for type II GnRH-R and antagonist for type I GnRH-R) was unable to elicit any cellular response tested in these pituitary cells. The GnRH-II responses were blocked by the type I GnRH-R-antagonists CRX or 135-18, suggesting that these effects were mediated by the type I GnRH-R. In contrast to pituitary cells, GnRH-I, but not GnRH-II, elicited an IP3 response in superovulated ovarian cells; 135-18 also had no effect. However, GnRH-II as well as GnRH-I presented antiproliferative effects on these cells. Surprisingly, 135-18 had stronger antiproliferative effects than either GnRH peptide. The 135-18 analog, but not GnRH-I or GnRH-II, increased progesterone secretion in superovulated ovarian cells. These results strongly suggest that GnRH-II is able to stimulate rat pituitary cells through the type I GnRH-R, with no evidence for the presence of type II GnRH-R. On the other hand, our results indicate a putative GnRH-R in superovulated ovarian cells with response characteristics that differ from those of the GnRH-R in the pituitary.

Registro:

Documento: Artículo
Título:Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells
Autor:Mongiat, L.A.; Lux-Lantos, V.A.; Libertun, C.
Filiación:Laboratorio de Neuroendocrinologia, Inst. de Biol. y Med. Exp. (IBYME), 1428 Buenos Aires, Argentina
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, Buenos Aires, Argentina
Palabras clave:Gonadotropin-releasing hormone; Gonadotropin-releasing hormone receptor; Neuroendocrinology; Ovary; Pituitary; gonadorelin; gonadorelin derivative; gonadorelin receptor; inositol trisphosphate; animal cell; article; cell proliferation; concentration response; controlled study; female; follitropin release; gonadotropin release; hormonal regulation; hormone action; hypophysis cell; luteinizing hormone release; neuroendocrinology; nonhuman; ovary cell; priority journal; progesterone release; rat; superovulation; Animals; Cells, Cultured; Female; Follicle Stimulating Hormone; Gonadotropin-Releasing Hormone; Hormone Antagonists; Luteinizing Hormone; Ovary; Pituitary Gland, Anterior; Rats; Rats, Sprague-Dawley; Receptors, LHRH; Superovulation; Animalia
Año:2004
Volumen:71
Número:2
Página de inicio:464
Página de fin:469
DOI: http://dx.doi.org/10.1095/biolreprod.104.027342
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:gonadorelin, 33515-09-2, 9034-40-6; inositol trisphosphate, 27121-73-9; cetrorelix, 120287-85-6; Follicle Stimulating Hormone, 9002-68-0; GNRHR2 protein, human; Gonadotropin-Releasing Hormone, 33515-09-2; Hormone Antagonists; Luteinizing Hormone, 9002-67-9; Receptors, LHRH
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v71_n2_p464_Mongiat

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Citas:

---------- APA ----------
Mongiat, L.A., Lux-Lantos, V.A. & Libertun, C. (2004) . Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells. Biology of Reproduction, 71(2), 464-469.
http://dx.doi.org/10.1095/biolreprod.104.027342
---------- CHICAGO ----------
Mongiat, L.A., Lux-Lantos, V.A., Libertun, C. "Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells" . Biology of Reproduction 71, no. 2 (2004) : 464-469.
http://dx.doi.org/10.1095/biolreprod.104.027342
---------- MLA ----------
Mongiat, L.A., Lux-Lantos, V.A., Libertun, C. "Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells" . Biology of Reproduction, vol. 71, no. 2, 2004, pp. 464-469.
http://dx.doi.org/10.1095/biolreprod.104.027342
---------- VANCOUVER ----------
Mongiat, L.A., Lux-Lantos, V.A., Libertun, C. Evidence for different gonadotropin-releasing hormone response sites in rat ovarian and pituitary cells. Biol. Reprod. 2004;71(2):464-469.
http://dx.doi.org/10.1095/biolreprod.104.027342