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Catecholestrogens are endogenous metabolites that have been shown to modulate granulosa, theca, and luteal cell function in some species. The present study was aimed at determining the possible role of these steroids on oocyte maturation. Cumulus-enclosed bovine oocytes were matured for 24 h, fertilized, and then cultured for 8 days. Whereas estradiol was without effect, addition of catecholestrogens (2-hydroxyestradiol, 4-hydroxyestradiol, and 2-methoxyestradiol [2-MOE2]) to the maturation medium did not affect the cleavage rate but was associated with a decrease in blastocyst production on Day 8. Although 2-MOE2 was also able to inhibit blastocyst formation when added during embryo culture, the effects were less pronounced than those seen when the steroid was added only during maturation. In agreement with the known ability of 2-MOE2 to bind tubulin at the colchicine site, marked alterations were observed in the spindle assembly of oocytes exposed to 2-MOE2 during maturation, which lead to gross chromosomal aberrations after fertilization and consequent developmental arrest at the morula stage. Moreover, that the blastocyst rate was not affected when meiosis was blocked with roscovitine during 2-MOE2 exposure is consistent with the idea that altered nuclear maturation is the cause of the low developmental competence. Because 2-MOE2 could be increased in follicular fluid in response to aryl hydrocarbon-receptor ligands, such as some environmental contaminants, our results show that abnormally high intraovarian levels of catecholestrogens could have a deleterious effect on oocyte maturation and early embryonic development arising from the alterations in the meiotic spindle.


Documento: Artículo
Título:Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development
Autor:Lattanzi, M.L.; Santos, C.B.; Mudry, M.D.; Baranao, J.L.
Filiación:Inst. de Biol. y Med. Experimental, Departamento de Ecología, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Quimica Biologica, Departamento de Ecología, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Grupo de Invest. en Biol. Evolutiva, Departamento de Ecología, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Embryo; Gamete biology; Meiosis; Oocyte development; Steroid hormones; 2 hydroxyestradiol; 2 methoxyestradiol; 4 hydroxyestradiol; aromatic hydrocarbon receptor; catechol estrogen; colchicine; estradiol; ligand; roscovitine; steroid; tubulin; animal cell; blastocyst; cattle; chromosome aberration; conference paper; controlled study; cumulus oophorus; embryo culture; embryo development; female; fertilization; gamete; in vitro study; male; meiosis; metabolite; microtubule assembly; nonhuman; oocyte cleavage; oocyte maturation; ovary follicle fluid; priority journal; Animals; Antioxidants; Apoptosis; Blastocyst; Cattle; Cells, Cultured; Colchicine; Cytogenetic Analysis; Dose-Response Relationship, Drug; Estradiol; Female; Fertilization in Vitro; Male; Meiosis; Mitotic Spindle Apparatus; Oocytes; Animalia; Bos taurus; Bovinae; Mammalia
Página de inicio:1793
Página de fin:1800
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
CAS:2 hydroxyestradiol, 362-05-0; 2 methoxyestradiol, 362-07-2; 4 hydroxyestradiol, 5976-61-4; colchicine, 64-86-8; estradiol, 50-28-2; roscovitine, 186692-46-6; 2-hydroxyestradiol, 362-05-0; 2-methoxyestradiol, 362-07-2; 4-hydroxyestradiol-17 beta, 5976-61-4; Antioxidants; Colchicine, 64-86-8; Estradiol, 50-28-2


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---------- APA ----------
Lattanzi, M.L., Santos, C.B., Mudry, M.D. & Baranao, J.L. (2003) . Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development. Biology of Reproduction, 69(6), 1793-1800.
---------- CHICAGO ----------
Lattanzi, M.L., Santos, C.B., Mudry, M.D., Baranao, J.L. "Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development" . Biology of Reproduction 69, no. 6 (2003) : 1793-1800.
---------- MLA ----------
Lattanzi, M.L., Santos, C.B., Mudry, M.D., Baranao, J.L. "Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development" . Biology of Reproduction, vol. 69, no. 6, 2003, pp. 1793-1800.
---------- VANCOUVER ----------
Lattanzi, M.L., Santos, C.B., Mudry, M.D., Baranao, J.L. Exposure of Bovine Oocytes to the Endogenous Metabolite 2-Methoxyestradiol during in Vitro Maturation Inhibits Early Embryonic Development. Biol. Reprod. 2003;69(6):1793-1800.