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Abstract:

Leptin, the 16-kDa protein product of the obese gene, was originally seen as an adipocyte-derived signaling molecule. Recently, it has been suggested to be involved in some functions during pregnancy, particularly in the placenta. In the present study, we investigated the role of leptin in the secretion of hCG, progesterone, and interleukin-6 (IL-6) by human term trophoblast cells in culture. Placentae were obtained from cesarean sections following uncomplicated pregnancies and used immediately after delivery. Leptin, hCG, progesterone, and IL-6 were measured by ELISA, RIA, and immunoradiometric assay in the cultured media of trophoblast cells cultured for 48 and 96 h. Leptin mRNA expression in these cultures was determined by reverse transcription-polymerase chain reaction. Recombinant human leptin added to primary cultures of human term placental trophoblast cells showed a stimulatory effect on hCG and IL-6 secretion and an inhibitory effect on progesterone secretion. Primary cultures of term trophoblast cells expressed leptin mRNA. All these findings suggest a role for leptin in human placental endocrine function.

Registro:

Documento: Artículo
Título:Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture
Autor:Cameo, P.; Bischof, P.; Calvo, J.C.
Filiación:Inst. de Biol./Medicina Exp. (IBYME), Vuelta de Obligado 2490, Buenos Aires, Argentina
Inst. de Biologia/Medicina Exp., Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Biological Chemistry, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Obstetrics, University of Geneva, Geneva, Switzerland
Palabras clave:Cytokines; Human chorionic gonadotropin; Leptin; Progesterone; Trophoblast; chorionic gonadotropin; interleukin 6; leptin; messenger RNA; progesterone; recombinant leptin; article; cell culture; controlled study; culture medium; cytokine release; endocrine function; enzyme linked immunosorbent assay; gene expression; gonadotropin release; hormone determination; human; human cell; immunoradiometric assay; placenta; priority journal; progesterone release; protein function; radioimmunoassay; regulatory mechanism; reverse transcription polymerase chain reaction; trophoblast; Cells, Cultured; Chorionic Gonadotropin; Delivery, Obstetric; Female; Humans; Interleukin-6; Leptin; Placenta; Pregnancy; Progesterone; Recombinant Proteins; Trophoblasts; Mammalia
Año:2003
Volumen:68
Número:2
Página de inicio:472
Página de fin:477
DOI: http://dx.doi.org/10.1095/biolreprod.102.006122
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:chorionic gonadotropin, 9002-61-3; progesterone, 57-83-0; Chorionic Gonadotropin; Interleukin-6; Leptin; Progesterone, 57-83-0; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v68_n2_p472_Cameo

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Citas:

---------- APA ----------
Cameo, P., Bischof, P. & Calvo, J.C. (2003) . Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture. Biology of Reproduction, 68(2), 472-477.
http://dx.doi.org/10.1095/biolreprod.102.006122
---------- CHICAGO ----------
Cameo, P., Bischof, P., Calvo, J.C. "Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture" . Biology of Reproduction 68, no. 2 (2003) : 472-477.
http://dx.doi.org/10.1095/biolreprod.102.006122
---------- MLA ----------
Cameo, P., Bischof, P., Calvo, J.C. "Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture" . Biology of Reproduction, vol. 68, no. 2, 2003, pp. 472-477.
http://dx.doi.org/10.1095/biolreprod.102.006122
---------- VANCOUVER ----------
Cameo, P., Bischof, P., Calvo, J.C. Effect of leptin on progesterone, human chorionic gonadotropin, and interleukin-6 secretion by human term trophoblast cells in culture. Biol. Reprod. 2003;68(2):472-477.
http://dx.doi.org/10.1095/biolreprod.102.006122