Artículo

Somoza, G.M.; Lescheid, D.W.; Miranda, L.A.; Lo Nostro, F.L.; Magliulo-Cepriano, L.; Montaner, A.D.; Schreibman, M.P.; Rivier, J.E.; Sherwood, N.M. "Expression of pejerrey gonadotropin-releasing hormone in three orders of fish" (2002) Biology of Reproduction. 67(6):1864-1871
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Abstract:

Molecular variants of GnRH were characterized by reversephase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]. Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish.

Registro:

Documento: Artículo
Título:Expression of pejerrey gonadotropin-releasing hormone in three orders of fish
Autor:Somoza, G.M.; Lescheid, D.W.; Miranda, L.A.; Lo Nostro, F.L.; Magliulo-Cepriano, L.; Montaner, A.D.; Schreibman, M.P.; Rivier, J.E.; Sherwood, N.M.
Filiación:Inst. Tecnologico de Chascomus, CONICET-Universidad de San Martin, Camino de Circunvalacion Laguna, Km 6. CC 164 (B7130IWA) Chascomus, Provincia de Buenos Aires, Argentina
Department of Biology, University of Victoria, Victoria, BC V8W 2Y2, Canada
Departamento de Ciencias Biologicas, Facultad Ciencias Exact. y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Aquatic Res./Environ. Assess. Ctr., Brooklyn Coll. City Univ. New York, New York, NY 11210, United States
Inst. de Investigaciones Biomedicas, Fundacion Pablo Cassara, Saladillo 2452 (C1440FFX), Buenos Aires, Argentina
Clayton Found. Labs. Peptide Biol., Salk Institute, San Diego, CA 92037, United States
Palabras clave:Anterior pituitary; Central nervous system; Gonadotropin-releasing hormone; Hypothalamic hormones; Neuropeptides; brain extract; gonadorelin; gonadorelin; tissue extract; animal tissue; article; chicken; controlled study; female; fish; hypophysis; hypophysis function; male; nonhuman; priority journal; radioimmunoassay; reversed phase high performance liquid chromatography; salmon; animal; brain level; chemistry; Cyprinodontiformes; gene expression; genetics; high performance liquid chromatography; isolation and purification; metabolism; Non-programmatic; Smegmamorpha; Anguilliformes; Animalia; Atheriniformes; Gallus gallus; Monopterus albus; Odontesthes bonariensis; Synbranchiformes; Synbranchus marmoratus; Xiphophorus hellerii; Xiphophorus maculatus; Non-programmatic; Animals; Brain Chemistry; Chromatography, High Pressure Liquid; Cyprinodontiformes; Female; Gene Expression; Gonadotropin-Releasing Hormone; Male; Pituitary Gland; Smegmamorpha; Tissue Extracts
Año:2002
Volumen:67
Número:6
Página de inicio:1864
Página de fin:1871
DOI: http://dx.doi.org/10.1095/biolreprod.102.004572
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:Gonadotropin-Releasing Hormone, 33515-09-2; Tissue Extracts
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n6_p1864_Somoza

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Citas:

---------- APA ----------
Somoza, G.M., Lescheid, D.W., Miranda, L.A., Lo Nostro, F.L., Magliulo-Cepriano, L., Montaner, A.D., Schreibman, M.P.,..., Sherwood, N.M. (2002) . Expression of pejerrey gonadotropin-releasing hormone in three orders of fish. Biology of Reproduction, 67(6), 1864-1871.
http://dx.doi.org/10.1095/biolreprod.102.004572
---------- CHICAGO ----------
Somoza, G.M., Lescheid, D.W., Miranda, L.A., Lo Nostro, F.L., Magliulo-Cepriano, L., Montaner, A.D., et al. "Expression of pejerrey gonadotropin-releasing hormone in three orders of fish" . Biology of Reproduction 67, no. 6 (2002) : 1864-1871.
http://dx.doi.org/10.1095/biolreprod.102.004572
---------- MLA ----------
Somoza, G.M., Lescheid, D.W., Miranda, L.A., Lo Nostro, F.L., Magliulo-Cepriano, L., Montaner, A.D., et al. "Expression of pejerrey gonadotropin-releasing hormone in three orders of fish" . Biology of Reproduction, vol. 67, no. 6, 2002, pp. 1864-1871.
http://dx.doi.org/10.1095/biolreprod.102.004572
---------- VANCOUVER ----------
Somoza, G.M., Lescheid, D.W., Miranda, L.A., Lo Nostro, F.L., Magliulo-Cepriano, L., Montaner, A.D., et al. Expression of pejerrey gonadotropin-releasing hormone in three orders of fish. Biol. Reprod. 2002;67(6):1864-1871.
http://dx.doi.org/10.1095/biolreprod.102.004572