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Abstract:

We evaluated the effects of transforming growth factor β1 (TGFβ1), alone or in combination with FSH and estradiol, on DNA synthesis in primary cultures of immature rat granulosa cells. 3H-Thymidine incorporation was significantly stimulated by TGFβ1 (5.6-fold). This effect was enhanced by FSH (20 ng/ml, 27.7-fold) or estradiol (100 ng/ml, 13.4-fold) or by a combination of both hormones (59.2-fold). Measurement of TGFβ bioactivity showed the presence of significant amounts of TGFβ in conditioned medium from granulosa cell cultures, and most of the activity was present in the latent form. FSH alone or in combination with estradiol produced a marked suppression of the production of latent and active TGFβ. Activated conditioned medium from control cultures of granulosa cell elicited a 1.4 fold increase in thymidine incorporation. This effect was markedly amplified by FSH (3-fold) and estradiol (4.3-fold) and by a combination of both (8.7-fold). The peptide containing the cell-binding domain of fibronectin (RGDSPC) partially inhibited thymidine incorporation stimulated by TGFβ1. Fibronectin did not synergize with FSH, and the interaction between TGFβ1 and FSH was even observed in the presence of this protein. The conclusions reached were as follows: 1) TGFβ1 is an autocrine stimulator of rat granulosa cell DNA synthesis, 2) FSH and estradiol produce a suppression of latent and active TGFβ production but markedly amplify TGFβ action, presumably at a postreceptor level, and 3) the stimulatory effects of TGFβ1 may be only partly mediated by the increased fibronectin secretion.

Registro:

Documento: Artículo
Título:Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
Autor:Saragüeta, P.E.; Lanuza, G.M.; Barañao, J.L.
Filiación:Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Estradiol; Follicle-stimulating hormone; Granulosa cells; Growth factors; estradiol; fibronectin; follitropin; thymidine; transforming growth factor beta1; arginyl-glycyl-aspartic acid; arginylglycylaspartic acid; DNA; estradiol; fibronectin; follitropin; oligopeptide; peptide fragment; Tgfb1 protein, rat; transforming growth factor beta; transforming growth factor beta1; animal cell; article; autocrine effect; cell proliferation; controlled study; DNA synthesis; female; granulosa cell; hormone action; nonhuman; priority journal; protein binding; protein domain; rat; animal; binding site; biosynthesis; cell culture; cell division; chemistry; culture medium; cytology; drug antagonism; drug effect; drug interaction; metabolism; physiology; Sprague Dawley rat; Animalia; Animals; Binding Sites; Cell Division; Cells, Cultured; Culture Media, Conditioned; DNA; Drug Interactions; Estradiol; Female; Fibronectins; Follicle Stimulating Hormone; Granulosa Cells; Oligopeptides; Peptide Fragments; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; Transforming Growth Factor beta1
Año:2002
Volumen:66
Número:6
Página de inicio:1862
Página de fin:1868
DOI: http://dx.doi.org/10.1095/biolreprod66.6.1862
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:estradiol, 50-28-2; fibronectin, 86088-83-7; follitropin, 9002-68-0; thymidine, 50-89-5; arginyl-glycyl-aspartic acid, 99896-85-2; Culture Media, Conditioned; DNA, 9007-49-2; Estradiol, 50-28-2; Fibronectins; Follicle Stimulating Hormone, 9002-68-0; Oligopeptides; Peptide Fragments; Tgfb1 protein, rat; Transforming Growth Factor beta; Transforming Growth Factor beta1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v66_n6_p1862_Saragueta

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Citas:

---------- APA ----------
Saragüeta, P.E., Lanuza, G.M. & Barañao, J.L. (2002) . Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation. Biology of Reproduction, 66(6), 1862-1868.
http://dx.doi.org/10.1095/biolreprod66.6.1862
---------- CHICAGO ----------
Saragüeta, P.E., Lanuza, G.M., Barañao, J.L. "Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation" . Biology of Reproduction 66, no. 6 (2002) : 1862-1868.
http://dx.doi.org/10.1095/biolreprod66.6.1862
---------- MLA ----------
Saragüeta, P.E., Lanuza, G.M., Barañao, J.L. "Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation" . Biology of Reproduction, vol. 66, no. 6, 2002, pp. 1862-1868.
http://dx.doi.org/10.1095/biolreprod66.6.1862
---------- VANCOUVER ----------
Saragüeta, P.E., Lanuza, G.M., Barañao, J.L. Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation. Biol. Reprod. 2002;66(6):1862-1868.
http://dx.doi.org/10.1095/biolreprod66.6.1862