Artículo

Cohen, D.J.; Ellerman, D.A.; Busso, D.; Morgenfeld, M.M.; Piazza, A.D.; Hayashi, M.; Young, E.T.; Kasahara, M.; Cuasnicu, P.S. "Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg" (2001) Biology of Reproduction. 65(4):1000-1005
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Abstract:

Human epididymal sperm protein ARP, a member of the cysteine-rich secretory proteins (CRISP) family, exhibits significant homology with rat epididymal protein DE, a candidate molecule for mediating sperm-egg fusion in rodents. The aim of this study was to investigate the involvement of ARP in human gamete fusion. Sequential extraction of proteins from ejaculated human sperm revealed the existence of a population of ARP that is tightly associated with the sperm surface and thus, potentially capable of participating in gamete interaction. Exposure of capacitated human sperm to a polyclonal antibody against recombinant ARP (anti-ARP) produced a significant and concentration-dependent inhibition in the ability of human sperm to penetrate zona-free hamster eggs. This inhibition was not due to a deleterious effect on the gametes because anti-ARP affected neither sperm viability or motility, nor egg penetrability. The antibody did not inhibit the occurrence of spontaneous or Ca 2+ ionophore-induced acrosome reaction, nor did it inhibit the ability of sperm to bind to the oolema, supporting a specific inhibition of the antibody at the sperm-egg fusion level. As a relevant evidence for a role of ARP in gamete fusion, the existence of complementary sites for this protein on the surface of human eggs was investigated. Experiments in which zona-free human oocytes discarded from in vitro fertilization programs were exposed to ARP, fixed, and subjected to indirect immunofluorescence revealed the presence of specific ARP-binding sites on the entire surface of the human egg, in agreement with the fusogenic properties of the human oolema. Together, these results strongly support the participation of ARP in the sperm-egg fusion process, suggesting that this protein would be the functional homologue of DE in humans.

Registro:

Documento: Artículo
Título:Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg
Autor:Cohen, D.J.; Ellerman, D.A.; Busso, D.; Morgenfeld, M.M.; Piazza, A.D.; Hayashi, M.; Young, E.T.; Kasahara, M.; Cuasnicu, P.S.
Filiación:Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Epididymis; Fertilization; Gamete biology; Ovum; Sperm; calcium ionophore; human epididymal sperm protein ARP; polyclonal antibody; rat epididymal sperm protein DE; recombinant protein; secretory protein; unclassified drug; acrosome reaction; animal cell; article; cell fusion; cell viability; concentration response; controlled study; extraction; female; fertilization; hamster; human; human cell; immunofluorescence; male; metabolic inhibition; nonhuman; normal human; oocyte; priority journal; sequence homology; sperm; spermatozoon capacitation; spermatozoon motility; spermatozoon penetration; surface property; zona pellucida
Año:2001
Volumen:65
Número:4
Página de inicio:1000
Página de fin:1005
DOI: http://dx.doi.org/10.1095/biolreprod65.4.1000
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v65_n4_p1000_Cohen

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Citas:

---------- APA ----------
Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M.M., Piazza, A.D., Hayashi, M., Young, E.T.,..., Cuasnicu, P.S. (2001) . Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg. Biology of Reproduction, 65(4), 1000-1005.
http://dx.doi.org/10.1095/biolreprod65.4.1000
---------- CHICAGO ----------
Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M.M., Piazza, A.D., Hayashi, M., et al. "Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg" . Biology of Reproduction 65, no. 4 (2001) : 1000-1005.
http://dx.doi.org/10.1095/biolreprod65.4.1000
---------- MLA ----------
Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M.M., Piazza, A.D., Hayashi, M., et al. "Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg" . Biology of Reproduction, vol. 65, no. 4, 2001, pp. 1000-1005.
http://dx.doi.org/10.1095/biolreprod65.4.1000
---------- VANCOUVER ----------
Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M.M., Piazza, A.D., Hayashi, M., et al. Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg. Biol. Reprod. 2001;65(4):1000-1005.
http://dx.doi.org/10.1095/biolreprod65.4.1000