Artículo

Marín-Briggiler, C.I.; Vazquez-Levin, M.H.; Gonzalez-Echeverría, F.; Blaquier, J.A.; Tezón, J.G.; Miranda, P.V. "Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction" (1999) Biology of Reproduction. 61(3):673-680
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Abstract:

The ability of strontium (Sr2+) to replace calcium (Ca2+) in maintaining human sperm function has still not been completely characterized. In the present study, acrosome reaction (AR) inducibility in response to human follicular fluid (hFF) was compared in spermatozoa incubated in either Ca2+- or Sr2+-containing media. Other events related to sperm capacitation, such as protein tyrosine phosphorylation and hyperactivation as well as zona pellucida (ZP) recognition under both conditions, were also analyzed. Spermatozoa incubated overnight in the presence of Sr2+ were unable to undergo the AR when exposed to hFF. Nevertheless, when spermatozoa were incubated under this condition and then transferred to medium with Ca2+, sperm response to hFF was similar to that of cells incubated throughout in the presence of Ca2+. The sperm protein tyrosine phosphorylation patterns and the percentages of sperm motility and hyperactivation were similar after incubation in Ca2+- or Sr2+-containing media. Under both conditions, the same binding capacity to homologous ZP was observed. Similar results were obtained when EGTA was added in order to chelate traces of Ca2+ present in Sr2+ medium. From these results, it can be concluded that Sr2+ can replace Ca2+ in supporting capacitation- related events and ZP binding, but not hFF-induced AR of human spermatozoa.

Registro:

Documento: Artículo
Título:Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction
Autor:Marín-Briggiler, C.I.; Vazquez-Levin, M.H.; Gonzalez-Echeverría, F.; Blaquier, J.A.; Tezón, J.G.; Miranda, P.V.
Filiación:Inst. de Biol. y Med. Experimental, (1428) Buenos Aires, Argentina
Fertilab, (1116) Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:protein tyrosine kinase; strontium; acrosome reaction; article; controlled study; human; human cell; ovary follicle fluid; priority journal; protein binding; protein phosphorylation; semen analysis; spermatozoon capacitation; spermatozoon motility; zona pellucida; Acrosome Reaction; Calcium; Egtazic Acid; Female; Follicular Fluid; Humans; Kinetics; Male; Phosphorylation; Phosphotyrosine; Sperm Capacitation; Sperm Motility; Sperm-Ovum Interactions; Spermatozoa; Strontium; Zona Pellucida
Año:1999
Volumen:61
Número:3
Página de inicio:673
Página de fin:680
DOI: http://dx.doi.org/10.1095/biolreprod61.3.673
Título revista:Biology of Reproduction
Título revista abreviado:Biol. Reprod.
ISSN:00063363
CODEN:BIREB
CAS:Calcium, 7440-70-2; Egtazic Acid, 67-42-5; Phosphotyrosine, 21820-51-9; Strontium, 7440-24-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v61_n3_p673_MarinBriggiler

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Citas:

---------- APA ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echeverría, F., Blaquier, J.A., Tezón, J.G. & Miranda, P.V. (1999) . Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction. Biology of Reproduction, 61(3), 673-680.
http://dx.doi.org/10.1095/biolreprod61.3.673
---------- CHICAGO ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echeverría, F., Blaquier, J.A., Tezón, J.G., Miranda, P.V. "Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction" . Biology of Reproduction 61, no. 3 (1999) : 673-680.
http://dx.doi.org/10.1095/biolreprod61.3.673
---------- MLA ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echeverría, F., Blaquier, J.A., Tezón, J.G., Miranda, P.V. "Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction" . Biology of Reproduction, vol. 61, no. 3, 1999, pp. 673-680.
http://dx.doi.org/10.1095/biolreprod61.3.673
---------- VANCOUVER ----------
Marín-Briggiler, C.I., Vazquez-Levin, M.H., Gonzalez-Echeverría, F., Blaquier, J.A., Tezón, J.G., Miranda, P.V. Strontium supports human sperm capacitation but not follicular fluid- induced acrosome reaction. Biol. Reprod. 1999;61(3):673-680.
http://dx.doi.org/10.1095/biolreprod61.3.673