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Abstract:

Background: Abnormalities in both thalamic and cortical areas have been reported in human cocaine addicts with noninvasive functional magnetic resonance imaging. Given the substantial involvement of the thalamocortical system in sensory processing and perception, we defined electrophysiology-based protocols to attempt a characterization of cocaine effects on thalamocortical circuits. Methods: Thalamocortical function was studied in vivo and in vitro in mice after cocaine "binge" administration. In vivo awake electroencephalography (EEG) was implemented in mice injected with saline, 1 hour or 24 hours after the last cocaine "binge" injection. In vitro current- and voltage-clamp whole-cell patch-clamp recordings were performed from slices including thalamic relay ventrobasal (VB) neurons. Results: In vivo EEG recordings after cocaine "binge" administration showed a significant increment, compared with saline, in low frequencies while observing no changes in high-frequency γ activity. In vitro patch recordings from VB neurons after cocaine "binge" administration showed low threshold spikes activation at more negative membrane potentials and increments in both lh and low voltage activated T-type calcium currents. Also, a 10-mV negative shift on threshold activation level of T-type current and a remarkable increment in both frequency and amplitudes of γ-aminobutyric acid-A-mediated minis were observed. Conclusions: Our data indicate that thalamocortical dysfunctions observed in cocaine abusers might be due to two distinct but additive events: 1) increased low frequency oscillatory thalamocortical activity, and 2) overinhibition of VB neurons that can abnormally "lock" the whole thalamocortical system at low frequencies. © 2009 Society of Biological Psychiatry.

Registro:

Documento: Artículo
Título:Cocaine acute "binge" administration results in altered thalamocortical interactions in mice
Autor:Urbano, F.J.; Bisagno, V.; Wikinski, S.I.; Uchitel, O.D.; Llinás, R.R.
Filiación:Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY, United States
Laboratorio de Fisiología y Biología Molecular (LFBM), Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Ciudad Universitaria, Buenos Aires, Argentina
Instituto de Investigaciones Farmacológicas (ININFA-UBACONICET), Buenos Aires, Argentina
Palabras clave:Cocaine; GABA-A receptors; Mice; T-type calcium channels; Thalamocortical dysrhythmia; 2 amino 5 phosphonovaleric acid; 4 aminobutyric acid A receptor; 6 cyano 7 nitro 2,3 quinoxalinedione; AMPA receptor antagonist; calcium channel T type; cesium; cocaine; kainic acid receptor antagonist; n methyl dextro aspartic acid receptor blocking agent; picrotoxin; potassium ion; tetrodotoxin; 4 aminobutyric acid A receptor; cocaine; animal cell; animal experiment; animal model; animal tissue; article; brain function; brain slice; cocaine dependence; drug abuse; electroencephalography; in vitro study; in vivo study; mouse; neurotransmitter release; nonhuman; patch clamp; priority journal; thalamocortical tract; voltage clamp; animal; brain cortex; C57BL mouse; cell membrane potential; drug antagonism; drug effect; drug interaction; drug potentiation; methodology; nerve cell; nerve cell inhibition; nerve tract; physiology; thalamus; Animals; Cerebral Cortex; Cocaine; Drug Interactions; Electroencephalography; Membrane Potentials; Mice; Mice, Inbred C57BL; Neural Inhibition; Neural Pathways; Neurons; Patch-Clamp Techniques; Receptors, GABA-A; Thalamus
Año:2009
Volumen:66
Número:8
Página de inicio:769
Página de fin:776
DOI: http://dx.doi.org/10.1016/j.biopsych.2009.04.026
Título revista:Biological Psychiatry
Título revista abreviado:Biol. Psychiatry
ISSN:00063223
CODEN:BIPCB
CAS:2 amino 5 phosphonovaleric acid, 76726-92-6; 6 cyano 7 nitro 2,3 quinoxalinedione, 115066-14-3; cesium, 7440-46-2; cocaine, 50-36-2, 53-21-4, 5937-29-1; picrotoxin, 124-87-8; potassium ion, 24203-36-9; tetrodotoxin, 4368-28-9, 4664-41-9; Cocaine, 50-36-2; Receptors, GABA-A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063223_v66_n8_p769_Urbano

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Citas:

---------- APA ----------
Urbano, F.J., Bisagno, V., Wikinski, S.I., Uchitel, O.D. & Llinás, R.R. (2009) . Cocaine acute "binge" administration results in altered thalamocortical interactions in mice. Biological Psychiatry, 66(8), 769-776.
http://dx.doi.org/10.1016/j.biopsych.2009.04.026
---------- CHICAGO ----------
Urbano, F.J., Bisagno, V., Wikinski, S.I., Uchitel, O.D., Llinás, R.R. "Cocaine acute "binge" administration results in altered thalamocortical interactions in mice" . Biological Psychiatry 66, no. 8 (2009) : 769-776.
http://dx.doi.org/10.1016/j.biopsych.2009.04.026
---------- MLA ----------
Urbano, F.J., Bisagno, V., Wikinski, S.I., Uchitel, O.D., Llinás, R.R. "Cocaine acute "binge" administration results in altered thalamocortical interactions in mice" . Biological Psychiatry, vol. 66, no. 8, 2009, pp. 769-776.
http://dx.doi.org/10.1016/j.biopsych.2009.04.026
---------- VANCOUVER ----------
Urbano, F.J., Bisagno, V., Wikinski, S.I., Uchitel, O.D., Llinás, R.R. Cocaine acute "binge" administration results in altered thalamocortical interactions in mice. Biol. Psychiatry. 2009;66(8):769-776.
http://dx.doi.org/10.1016/j.biopsych.2009.04.026