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Abstract:

a-1,4-glucan:a-1,4-glucan 6-glycosyltransferase was purified about 35-fold from rat-liver extracts. Removal of α-amylase could be accomplished by high-speed centrifugation of extracts from livers with high glycogen content. Enzyme action on amylopectin is optimum at pH 6.4 in 0.3 M citrate buffer. It requires salts for maximal activity, and is inhibited by molybdate, Mn2+, Mg2+ and sodium p-chloromercuribenzoate. Amylose and amylopectin ß-limit dextrin were also substrates for the liver branching enzyme. A method is described for the assay of the enzyme in the presence of α-amylase. © 1962.

Registro:

Documento: Artículo
Título:a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver
Autor:Krisman, C.R.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:glucosyltransferase; article; liver; GLUCOSYLTRANSFERASES; LIVER; Glucosyltransferases; Liver
Año:1962
Volumen:65
Número:2
Página de inicio:307
Página de fin:315
DOI: http://dx.doi.org/10.1016/0006-3002(62)91049-1
Título revista:BBA - Biochimica et Biophysica Acta
ISSN:00063002
CAS:glucosyltransferase, 9031-48-5; Glucosyltransferases, 2.4.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063002_v65_n2_p307_Krisman

Citas:

---------- APA ----------
(1962) . a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver. BBA - Biochimica et Biophysica Acta, 65(2), 307-315.
http://dx.doi.org/10.1016/0006-3002(62)91049-1
---------- CHICAGO ----------
Krisman, C.R. "a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver" . BBA - Biochimica et Biophysica Acta 65, no. 2 (1962) : 307-315.
http://dx.doi.org/10.1016/0006-3002(62)91049-1
---------- MLA ----------
Krisman, C.R. "a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver" . BBA - Biochimica et Biophysica Acta, vol. 65, no. 2, 1962, pp. 307-315.
http://dx.doi.org/10.1016/0006-3002(62)91049-1
---------- VANCOUVER ----------
Krisman, C.R. a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver. 1962;65(2):307-315.
http://dx.doi.org/10.1016/0006-3002(62)91049-1