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Abstract:

We report a resonance Raman and UV-vis characterization of the active site structure of oxidatively modified forms of cytochrome c (Cyt-c) free in solution and in complexes with cardiolipin (CL). The studied post-translational modifications of Cyt-c include methionine sulfoxidation and tyrosine nitration, which lead to altered heme axial ligation and increased peroxidase activity with respect to those of the wild-type protein. In spite of the structural and activity differences between the protein variants free in solution, binding to CL liposomes induces in all cases the formation of a spectroscopically identical bis-His axial coordination conformer that more efficiently promotes lipid peroxidation. The spectroscopic results indicate that the bis-His form is in equilibrium with small amounts of high-spin species, thus suggesting a labile distal His ligand as the basis for the CL-induced increase in enzymatic activity observed for all protein variants. For Cyt-c nitrated at Tyr74 and sulfoxidized at Met80, the measured apparent binding affinities for CL are ∼4 times larger than for wild-type Cyt-c. On the basis of these results, we propose that these post-translational modifications may amplify the pro-apoptotic signal of Cyt-c under oxidative stress conditions at CL concentrations lower than for the unmodified protein. © 2015 American Chemical Society.

Registro:

Documento: Artículo
Título:Active Site Structure and Peroxidase Activity of Oxidatively Modified Cytochrome c Species in Complexes with Cardiolipin
Autor:Capdevila, D.A.; Oviedo Rouco, S.; Tomasina, F.; Tortora, V.; Demicheli, V.; Radi, R.; Murgida, D.H.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física and INQUIMAE (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, piso 1, Buenos Aires, C1428EHA, Argentina
Departamento de Bioquímica, Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Palabras clave:Amino acids; Binding energy; Bins; Enzyme activity; Liposomes; Nitration; Oxidation; Phospholipids; Active site structure; Enzymatic activities; Lipid peroxidation; Peroxidase activities; Post-translational modifications; Proapoptotic signals; Tyrosine nitration; Wild-type proteins; Proteins; cardiolipin; cytochrome c; heme; liposome; methionine; peroxidase; protein variant; tyrosine; cardiolipin; cytochrome c; Article; conformational transition; controlled study; enzyme active site; enzyme activity; enzyme structure; excitation; lipid peroxidation; nitration; oxidative stress; priority journal; protein processing; protein protein interaction; Raman spectrometry; sulfoxidation; ultracentrifugation; ultraviolet spectroscopy; animal; chemistry; enzyme active site; horse; protein conformation; ultraviolet spectrophotometry; Animals; Cardiolipins; Catalytic Domain; Cytochromes c; Horses; Protein Conformation; Spectrophotometry, Ultraviolet; Spectrum Analysis, Raman
Año:2015
Volumen:54
Número:51
Página de inicio:7491
Página de fin:7504
DOI: http://dx.doi.org/10.1021/acs.biochem.5b00922
Título revista:Biochemistry
Título revista abreviado:Biochemistry
ISSN:00062960
CODEN:BICHA
CAS:cytochrome c, 9007-43-6, 9064-84-0; heme, 14875-96-8; methionine, 59-51-8, 63-68-3, 7005-18-7; peroxidase, 9003-99-0; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Cardiolipins; Cytochromes c
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v54_n51_p7491_Capdevila

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Citas:

---------- APA ----------
Capdevila, D.A., Oviedo Rouco, S., Tomasina, F., Tortora, V., Demicheli, V., Radi, R. & Murgida, D.H. (2015) . Active Site Structure and Peroxidase Activity of Oxidatively Modified Cytochrome c Species in Complexes with Cardiolipin. Biochemistry, 54(51), 7491-7504.
http://dx.doi.org/10.1021/acs.biochem.5b00922
---------- CHICAGO ----------
Capdevila, D.A., Oviedo Rouco, S., Tomasina, F., Tortora, V., Demicheli, V., Radi, R., et al. "Active Site Structure and Peroxidase Activity of Oxidatively Modified Cytochrome c Species in Complexes with Cardiolipin" . Biochemistry 54, no. 51 (2015) : 7491-7504.
http://dx.doi.org/10.1021/acs.biochem.5b00922
---------- MLA ----------
Capdevila, D.A., Oviedo Rouco, S., Tomasina, F., Tortora, V., Demicheli, V., Radi, R., et al. "Active Site Structure and Peroxidase Activity of Oxidatively Modified Cytochrome c Species in Complexes with Cardiolipin" . Biochemistry, vol. 54, no. 51, 2015, pp. 7491-7504.
http://dx.doi.org/10.1021/acs.biochem.5b00922
---------- VANCOUVER ----------
Capdevila, D.A., Oviedo Rouco, S., Tomasina, F., Tortora, V., Demicheli, V., Radi, R., et al. Active Site Structure and Peroxidase Activity of Oxidatively Modified Cytochrome c Species in Complexes with Cardiolipin. Biochemistry. 2015;54(51):7491-7504.
http://dx.doi.org/10.1021/acs.biochem.5b00922