Artículo

Arroyo Mañez, P.; Lu, C.; Boechi, L.; Martí, M.A.; Shepherd, M.; Wilson, J.L.; Poole, R.K.; Luque, F.J.; Yeh, S.-R.; Estrin, D.A. "Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni" (2011) Biochemistry. 50(19):3946-3956
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Abstract:

Oxygen affinity in heme-containing proteins is determined by a number of factors, such as the nature and conformation of the distal residues that stabilize the heme bound-oxygen via hydrogen-bonding interactions. The truncated hemoglobin III from Campylobacter jejuni (Ctb) contains three potential hydrogen-bond donors in the distal site: TyrB10, TrpG8, and HisE7. Previous studies suggested that Ctb exhibits an extremely slow oxygen dissociation rate due to an interlaced hydrogen-bonding network involving the three distal residues. Here we have studied the structural and kinetic properties of the G8WF mutant of Ctb and employed state-of-the-art computer simulation methods to investigate the properties of the O2 adduct of the G8 WF mutant, with respect to those of the wild-type protein and the previously studied E7HL and/or B10YF mutants. Our data indicate that the unique oxygen binding properties of Ctb are determined by the interplay of hydrogen-bonding interactions between the heme-bound ligand and the surrounding TyrB10, TrpG8, and HisE7 residues. © 2011 American Chemical Society.

Registro:

Documento: Artículo
Título:Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni
Autor:Arroyo Mañez, P.; Lu, C.; Boechi, L.; Martí, M.A.; Shepherd, M.; Wilson, J.L.; Poole, R.K.; Luque, F.J.; Yeh, S.-R.; Estrin, D.A.
Filiación:Departamento de Química Inorgánica, Analítica, y Química Fisica, INQUIMAE-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, United States
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom
Department de Fisicoquimica and Institut de Biomedicina (IBUB), Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain
Palabras clave:Campylobacter jejuni; Dissociation rates; Hydrogen bonding interactions; Hydrogen bonding network; Hydrogen-bond donors; Kinetic properties; Oxygen affinity; Oxygen binding; Truncated hemoglobins; Wild-type proteins; Binding energy; Computer simulation; Dissociation; Hemoglobin; Hydrogen; Hydrogen bonds; Porphyrins; Oxygen; truncated hemoglobin; article; Campylobacter jejuni; computer simulation; hydrogen bond; molecular dynamics; oxygen affinity; priority journal; Raman spectrometry; Bacterial Proteins; Campylobacter jejuni; Glycine; Heme; Histidine; Hydrogen Bonding; Ligands; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Oxygen; Protein Binding; Spectrum Analysis, Raman; Truncated Hemoglobins; Tryptophan; Tyrosine; Campylobacter jejuni
Año:2011
Volumen:50
Número:19
Página de inicio:3946
Página de fin:3956
DOI: http://dx.doi.org/10.1021/bi101137n
Título revista:Biochemistry
Título revista abreviado:Biochemistry
ISSN:00062960
CODEN:BICHA
CAS:Bacterial Proteins; Glycine, 56-40-6; Heme, 14875-96-8; Histidine, 71-00-1; Ligands; Oxygen, 7782-44-7; Truncated Hemoglobins; Tryptophan, 73-22-3; Tyrosine, 55520-40-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v50_n19_p3946_ArroyoManez

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Citas:

---------- APA ----------
Arroyo Mañez, P., Lu, C., Boechi, L., Martí, M.A., Shepherd, M., Wilson, J.L., Poole, R.K.,..., Estrin, D.A. (2011) . Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni. Biochemistry, 50(19), 3946-3956.
http://dx.doi.org/10.1021/bi101137n
---------- CHICAGO ----------
Arroyo Mañez, P., Lu, C., Boechi, L., Martí, M.A., Shepherd, M., Wilson, J.L., et al. "Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni" . Biochemistry 50, no. 19 (2011) : 3946-3956.
http://dx.doi.org/10.1021/bi101137n
---------- MLA ----------
Arroyo Mañez, P., Lu, C., Boechi, L., Martí, M.A., Shepherd, M., Wilson, J.L., et al. "Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni" . Biochemistry, vol. 50, no. 19, 2011, pp. 3946-3956.
http://dx.doi.org/10.1021/bi101137n
---------- VANCOUVER ----------
Arroyo Mañez, P., Lu, C., Boechi, L., Martí, M.A., Shepherd, M., Wilson, J.L., et al. Role of the distal hydrogen-bonding network in regulating oxygen affinity in the truncated hemoglobin III from campylobacter jejuni. Biochemistry. 2011;50(19):3946-3956.
http://dx.doi.org/10.1021/bi101137n