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Di Lella, S.; Martí, M.A.; Croci, D.O.; Guardia, C.M.A.; Díaz-Ricci, J.C.; Rabinovich, G.A.; Caramelo, J.J.; Estrin, D.A. "Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria" (2010) Biochemistry. 49(35):7652-7658
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Abstract:

The stability of proteins involves a critical balance of interactions of different orders of magnitude. In this work, we present experimental evidence of an increased thermal stability of galectin-1, a multifunctional β-galactoside-binding protein, upon binding to the disaccharide lactose. Analysis of structural changes occurring upon binding of lectin to its specific glycans and thermal denaturation of the protein and the complex were analyzed by circular dichroism. On the other hand, we studied dimerization as another factor that may induce structural and thermal stability changes. The results were then complemented with molecular dynamics simulations followed by a detailed computation of thermodynamic properties, including the internal energy, solvation free energy, and conformational entropy. In addition, an energetic profile of the binding and dimerization processes is also presented. Whereas binding and cross-linking of lactose do not alter galectin-1 structure, this interaction leads to substantial changes in the flexibility and internal energy of the protein which confers increased thermal stability to this endogenous lectin. Given that an improved understanding of the physicochemical properties of galectin-glycan lattices may contribute to the dissection of their biological functions and prediction of their therapeutic applications, our study suggests that galectin binding to specific disaccharide ligands may increase the thermal stability of this glycan-binding protein, an effect that could influence its critical biological functions. © 2010 American Chemical Society.

Registro:

Documento: Artículo
Título:Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria
Autor:Di Lella, S.; Martí, M.A.; Croci, D.O.; Guardia, C.M.A.; Díaz-Ricci, J.C.; Rabinovich, G.A.; Caramelo, J.J.; Estrin, D.A.
Filiación:Departamento de Química Inorgánica, Analítica y Química-Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, CONICET, Ciudad de Buenos Aires, Argentina
Instituto Superior de Investigaciones Biológicas, CONICET-Facultad de Bioquímica, Universidad Nacional de Tucumán, San Miguel de Tucuman, Argentina
Fundación Instituto Leloir, Ciudad de Buenos Aires, Argentina
Palabras clave:Biological systems; Dichroism; Dimerization; Ligands; Molecular dynamics; Proteins; Sugars; Thermodynamic stability; Binding proteins; Biological functions; Circular dichroism; Conformational entropy; Different order; Disaccharide lactose; Experimental evidence; Galectin-1; Glycans; Internal energies; Ligand binding; Molecular dynamics simulations; Physicochemical property; Solvation free energies; Structural change; Therapeutic Application; Thermal denaturations; Thermal stability; Binding energy; beta galactoside; carbohydrate binding protein; disaccharide; galectin 1; glycan; lactose; lectin; recombinant protein; beta-galactoside; galactoside; galectin 1; ligand; article; bacterial cell; circular dichroism; dimerization; energy; entropy; Escherichia coli; human; ligand binding; molecular dynamics; nonhuman; priority journal; protein denaturation; protein structure; spectroscopy; structure analysis; thermodynamics; thermostability; binding site; chemical structure; chemistry; metabolism; protein folding; Binding Sites; Dimerization; Galactosides; Galectin 1; Humans; Ligands; Models, Molecular; Protein Folding; Thermodynamics
Año:2010
Volumen:49
Número:35
Página de inicio:7652
Página de fin:7658
DOI: http://dx.doi.org/10.1021/bi100356g
Título revista:Biochemistry
Título revista abreviado:Biochemistry
ISSN:00062960
CODEN:BICHA
CAS:galectin 1, 258495-34-0; lactose, 10039-26-6, 16984-38-6, 63-42-3, 64044-51-5; beta-galactoside; Galactosides; Galectin 1; Ligands
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v49_n35_p7652_DiLella

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Citas:

---------- APA ----------
Di Lella, S., Martí, M.A., Croci, D.O., Guardia, C.M.A., Díaz-Ricci, J.C., Rabinovich, G.A., Caramelo, J.J.,..., Estrin, D.A. (2010) . Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria. Biochemistry, 49(35), 7652-7658.
http://dx.doi.org/10.1021/bi100356g
---------- CHICAGO ----------
Di Lella, S., Martí, M.A., Croci, D.O., Guardia, C.M.A., Díaz-Ricci, J.C., Rabinovich, G.A., et al. "Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria" . Biochemistry 49, no. 35 (2010) : 7652-7658.
http://dx.doi.org/10.1021/bi100356g
---------- MLA ----------
Di Lella, S., Martí, M.A., Croci, D.O., Guardia, C.M.A., Díaz-Ricci, J.C., Rabinovich, G.A., et al. "Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria" . Biochemistry, vol. 49, no. 35, 2010, pp. 7652-7658.
http://dx.doi.org/10.1021/bi100356g
---------- VANCOUVER ----------
Di Lella, S., Martí, M.A., Croci, D.O., Guardia, C.M.A., Díaz-Ricci, J.C., Rabinovich, G.A., et al. Linking the structure and thermal stability of β-galactoside-binding protein galectin-1 to ligand binding and dimerization equilibria. Biochemistry. 2010;49(35):7652-7658.
http://dx.doi.org/10.1021/bi100356g