Abstract:
Since the discovery of soluble guanylate cyclase (sGC) as the mammalian receptor for nitric oxide (NO), numerous studies have been performed in order to understand how sGC transduces the NO signal. However, the structural basis of sGC activation is still not completely elucidated. Spectroscopic and kinetic studies showed that the key step in the activation mechanism was the NO-induced breaking of the iron proximal histidine bond in the so-called 6c-NO to 5c-NO transition. The main breakthrough in the understanding of sGC activation mechanism came, however, from the elucidation of crystal structures for two different prokaryotic heme NO oxygen (HNOX) domains, which are homologues to the sGC heme domain. In this work we present computer simulation results of Thermoanaerobacter tencogensis HNOX that complement these structural studies, yielding molecular explanations to several poorly understood properties of these proteins. Specifically, our results explain the differential ligand binding patterns of the HNOX domains according to the nature of proximal and distal residues. We also show that the natural dynamics of these proteins is intimately related with the proposed conformational dependent activation process, which involves mainly the αFβ1 loop and the αA-αC distal subdomain. The results from the sGC models also support this view and suggest a key role for the αFβ1 loop in the iron proximal histidine bond breaking process and, therefore, in the sGC activation mechanism. © 2008 American Chemical Society.
Registro:
Documento: |
Artículo
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Título: | Dynamical characterization of the heme NO oxygen binding (HNOX) domain. Insight into soluble guanylate cyclase allosteric transition |
Autor: | Capece, L.; Estrin, D.A.; Marti, M.A. |
Filiación: | Departamento de Quimica Inorganica, Analitica Y Quimica Fisica/INQUIMAE-CONICET, Facultad de Ciencias Exactas Y Naturales, Pabellón 2, Buenos Aires, C1428EHA, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Pabellón 2, Buenos Aires, C1428EHA, Argentina
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Palabras clave: | Biochemistry; Computational methods; Computer networks; Computer simulation; Mammals; Mechanisms; Nitric oxide; Oxygen; Proteins; Activation mechanisms; Soluble guanylate cyclase; Hemoglobin; guanylate cyclase; hemoprotein; histidine; allosterism; article; computer simulation; enzyme activation; heme nitric oxide oxygen binding; ligand binding; priority journal; protein conformation; protein domain; protein structure; spectroscopy; Thermoanaerobacter; thermoanaerobacter tencogensis; Allosteric Regulation; Animals; Bacterial Proteins; Computer Simulation; Enzyme Activation; Guanylate Cyclase; Heme; Histidine; Humans; Iron; Models, Molecular; Nitric Oxide; Oxygen; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Signal Transduction; Structure-Activity Relationship; Thermoanaerobacter; Mammalia; Prokaryota; Thermoanaerobacter |
Año: | 2008
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Volumen: | 47
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Número: | 36
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Página de inicio: | 9416
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Página de fin: | 9427
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DOI: |
http://dx.doi.org/10.1021/bi800682k |
Título revista: | Biochemistry
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Título revista abreviado: | Biochemistry
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ISSN: | 00062960
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CODEN: | BICHA
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CAS: | guanylate cyclase, 9054-75-5; histidine, 645-35-2, 7006-35-1, 71-00-1; Bacterial Proteins; Guanylate Cyclase, EC 4.6.1.2; Heme, 14875-96-8; Histidine, 71-00-1; Iron, 7439-89-6; Nitric Oxide, 10102-43-9; Oxygen, 7782-44-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v47_n36_p9416_Capece |
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Citas:
---------- APA ----------
Capece, L., Estrin, D.A. & Marti, M.A.
(2008)
. Dynamical characterization of the heme NO oxygen binding (HNOX) domain. Insight into soluble guanylate cyclase allosteric transition. Biochemistry, 47(36), 9416-9427.
http://dx.doi.org/10.1021/bi800682k---------- CHICAGO ----------
Capece, L., Estrin, D.A., Marti, M.A.
"Dynamical characterization of the heme NO oxygen binding (HNOX) domain. Insight into soluble guanylate cyclase allosteric transition"
. Biochemistry 47, no. 36
(2008) : 9416-9427.
http://dx.doi.org/10.1021/bi800682k---------- MLA ----------
Capece, L., Estrin, D.A., Marti, M.A.
"Dynamical characterization of the heme NO oxygen binding (HNOX) domain. Insight into soluble guanylate cyclase allosteric transition"
. Biochemistry, vol. 47, no. 36, 2008, pp. 9416-9427.
http://dx.doi.org/10.1021/bi800682k---------- VANCOUVER ----------
Capece, L., Estrin, D.A., Marti, M.A. Dynamical characterization of the heme NO oxygen binding (HNOX) domain. Insight into soluble guanylate cyclase allosteric transition. Biochemistry. 2008;47(36):9416-9427.
http://dx.doi.org/10.1021/bi800682k