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Ellison, M.; Haberlandt, C.; Gomez-Casati, M.E.; Watkins, M.; Elgoyhen, A.B.; McIntosh, J.M.; Olivera, B.M. "α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR" (2006) Biochemistry. 45(5):1511-1517
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Abstract:

The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits assemble to form the α9α10 nAChR subtype. This receptor is believed to mediate cholinergic synaptic transmission between efferent olivocochlear fibers and the hair cells of the cochlea. In addition α9 and/or α10 expression has been described in dorsal root ganglion neurons, lymphocytes, skin keratinocytes, and the pars tuberalis of the pituitary. Specific antagonists that selectively block the α9α10 channel could be valuable tools for elucidating its role in these diverse tissues. This study describes a novel α-conotoxin from the Western Atlantic species Conus regius, α-conotoxin RgIA (α-RgIA), that is a subtype specific blocker of the α9α10 nAChR. α-RgIA belongs to the α4/3 subfamily of the α-conotoxin family; sequence and subtype specificity comparisons between α-RgIA and previously characterized α4/3 toxins indicate that the amino acids in the C-terminal half of α-RgIA are responsible for its preferential inhibition of the α9α10 nAChR subtype. © 2006 American Chemical Society.

Registro:

Documento: Artículo
Título:α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR
Autor:Ellison, M.; Haberlandt, C.; Gomez-Casati, M.E.; Watkins, M.; Elgoyhen, A.B.; McIntosh, J.M.; Olivera, B.M.
Filiación:Department of Biology, University of Utah, Salt Lake City, UT 84112, United States
Department of Pathology, University of Utah, Salt Lake City, UT 84112, United States
Department of Psychiatry, University of Utah, Salt Lake City, UT 84112, United States
Instituto Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112-0840, United States
Palabras clave:Amino acids; Biochemistry; Neurology; Skin; Tissue; Conotoxin; Dorsal root ganglion neurons; Lymphocytes; Nicotinic acetylcholine receptor (nAChR); Cells; alpha conotoxin RgIA; alpha10 nicotinic receptor; alpha9 alpha10 nicotinic receptor; alpha9 nicotinic receptor; amino acid; conotoxin; nicotinic receptor; nicotinic receptor blocking agent; receptor subtype; receptor subunit; unclassified drug; animal cell; animal tissue; article; carboxy terminal sequence; cholinergic transmission; cochlea; controlled study; Conus regius; efferent nerve; hair cell; hypophysis pars tuberalis; keratinocyte; lymphocyte; nonhuman; priority journal; protein assembly; protein expression; rat; receptor blocking; snail; spinal ganglion; toxin structure; Animals; Base Sequence; Cloning, Molecular; Conotoxins; Gene Expression Regulation; Hair Cells, Inner; Molecular Sequence Data; Oocytes; Polymerase Chain Reaction; Protein Subunits; Rats; Rats, Sprague-Dawley; Receptors, Nicotinic; Species Specificity; Xenopus; Animalia; Conus regius; Gastropoda
Año:2006
Volumen:45
Número:5
Página de inicio:1511
Página de fin:1517
DOI: http://dx.doi.org/10.1021/bi0520129
Título revista:Biochemistry
Título revista abreviado:Biochemistry
ISSN:00062960
CODEN:BICHA
CAS:amino acid, 65072-01-7; alpha10 acetylcholine receptor, rat; Chrna9 protein, rat; conotoxin alpha-RgIA, Conus regius; Conotoxins; Protein Subunits; Receptors, Nicotinic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v45_n5_p1511_Ellison

Referencias:

  • Armishaw, C.J., Alewood, P.F., Conotoxins as research tools and drug leads (2005) Curr. Protein Pept. Sci., 6, pp. 221-240
  • Wang, C.Z., Chi, C.W., Conus peptides - A rich pharmaceutical treasure (2004) Acta Biochim. Biophys. Sin., 36, pp. 713-723
  • Livett, B.G., Gayler, K.R., Kahlil, Z., Drugs from the sea: Conopeptides as potential therapeutics (2004) Curr. Med. Chem., 11, pp. 1715-1723
  • Lewis, R.J., Conotoxins as selective inhibitors of neuronal ion channels, receptors and transporters (2004) IUBMB Life, 56, pp. 89-93
  • Terlau, H., Olivera, B.M., Conus venoms: A rich source of novel ion channel-targeted peptides (2004) Physiol. Rev., 84, pp. 41-68
  • McIntosh, J.M., Santos, A.D., Olivera, B.M., Conus peptides targeted to specific nicotinic acetylcholine receptor subtypes (1999) Annu. Rev. Biochem., 68, pp. 59-88
  • Janes, R.W., Conotoxins as selective probes for nicotinic acetylcholine receptor subclasses (2005) Curr. Opin. Pharmacol, 5, pp. 280-292
  • Dutton, J.L., Craik, D.J., α-conotoxins: Nicotinic acetylcholine receptor antagonists as pharmacological tools and potential drug leads (2001) Curr. Med. Chem., 8, pp. 327-344
  • Arias, H.R., Blanton, M.P., Alpha-conotoxins (2000) Int. J. Biochem. Cell Biol., 32, pp. 1017-1028
  • Karlin, A., Emerging structure of the nicotinic acetylcholine receptors (2002) Nat. Rev. Neurosci., 3, pp. 102-114
  • Gotti, C., Clementi, F., Neuronal nicotinic receptors: From structure to pathology (2004) Prog. Neurobiol., 74, pp. 363-396
  • Le Novere, N., Corringer, P.J., Changeux, J.P., The diversity of subunit composition in nAChRs: Evolutionary origins, physiologic and pharmacologic consequences (2002) J. Neurobiol., 53, pp. 447-456
  • Sgard, F., Charpantier, E., Bertrand, S., Walker, N., Caput, D., Graham, D., Bertrand, D., Besnard, F., A Novel Human Nicotinic Receptor Subunit, α10, That Confers Functionality to the α9-Subunit (2002) Mol. Pharmacol., 61, pp. 150-159
  • Elgoyhen, A.B., Johnson, D.S., Boulter, J., Vetter, D.E., Heinemann, S., α9: An acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells (1994) Cell, 79, pp. 705-715
  • Elgoyhen, A.B., Vetter, D.E., Katz, E., Rothlin, C.V., Heinemann, S.F., Boulter, J., α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells (2001) Proc. Natl. Acad. Sci. U.S.A., 98, pp. 3501-3506
  • Haberberger, R.V., Bernardini, N., Kress, M., Hartmann, P., Lips, K.S., Kummer, W., Nicotinic acetylcholine receptor subtypes in nociceptive dorsal root ganglion neurons of the adult rat (2004) Auton. Neurosci., 113, pp. 32-42
  • Lips, K.S., Pfeil, U., Kummer, W., Coexpression of α9 and α10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons (2002) Neuroscience, 115, pp. 1-5
  • Peng, H., Ferris, R.L., Matthews, T., Hiel, H., Lopez-Albaitero, A., Lustig, L.R., Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes (2004) Life Sci., 76, pp. 263-280
  • Arredondo, J., Nguyen, V., Chernyavsky, A.I., Bercovich, D., Orr-Urtreger, A., Kummer, W., Lips, K., Grando, S.A., Central role of alpha7 nicotinic receptor in differentiation of the stratified squamous epithelium (2002) J. Cell Biol., 159, pp. 325-336
  • Nguyen, V.T., Ndoye, A., Grando, S.A., Novel human alpha9 acetylcholine receptor regulating keratinocyte adhesion is targeted by Pemphigus vulgaris autoimmunity (2000) Am. J. Pathol., 157, pp. 1377-1391
  • Kurzen, H., Berger, H., Jager, C., Hartschuh, W., Naher, H., Gratchev, A., Goerdt, S., Deichmann, M., Phenotypical and molecular profiling of the extraneuronal cholinergic system of the skin (2004) J. Invest. Dermatol., 123, pp. 937-949
  • Ellison, M., McIntosh, J.M., Olivera, B.M., α-conotoxins ImI and ImII: Similar α7 nicotinic receptor antagonists act at different sites (2003) J. Biol. Chem., 278, pp. 757-764
  • McIntosh, J.M., Plazas, P.V., Watkins, M., Gomez-Casati, M.E., Olivera, B.M., Elgoyhen, A.B., A novel α-conotoxin, PeIA, cloned from Conus pergrandis, discriminates between rat α9α10 and α7 nicotinic cholinergic receptors (2005) J. Biol. Chem., 280, pp. 30107-30112
  • Luo, S., Nguyen, T.A., Cartier, G.E., Olivera, B.M., Yoshikami, D., McIntosh, J.M., Single-residue alteration in α-conotoxin PnIA switches its nAChR subtype selectivity (1999) Biochemistry, 38, pp. 14542-14548
  • McIntosh, J.M., Yoshikami, D., Mahe, E., Nielsen, D.B., Rivier, J.E., Gray, W.R., Olivera, B.M., A nicotinic acetylcholine receptor ligand of unique specificity, α-conotoxin ImI (1994) J. Biol. Chem., 269, pp. 16733-16739
  • Cartier, G.E., Yoshikami, D., Gray, W.R., Luo, S., Olivera, B.M., McIntosh, J.M., A new α-conotoxin which targets α3β2 nicotinic acetylcholine receptors (1996) J. Biol. Chem., 271, pp. 7522-7528
  • Dowell, C., Olivera, B.M., Garrett, J.E., Staheli, S.T., Watkins, M., Kuryatov, A., Yoshikami, D., Mcintosh, J.M., α-conotoxin PIA is selective for α6 subunit-containing nicotinic acetylcholine receptors (2003) J. Neurosci., 23, pp. 8445-8452
  • McIntosh, J.M., Gardner, S., Luo, S., Garrett, J.E., Yoshikami, D., Conus peptides: Novel probes for nicotinic acetylcholine receptor structure and function (2000) Eur. J. Pharmacol., 393, pp. 205-208
  • Katz, E., Elgoyhen, A.B., Gomez-Casati, M.E., Knipper, M., Vetter, D.E., Fuchs, P.A., Glowatzki, E., Developmental regulation of nicotinic synapses on cochlear inner hair cells (2004) J. Neurosci., 24, pp. 7814-7820
  • Glowatzki, E., Fuchs, P.A., Cholinergic synaptic inhibition of inner hair cells in the neonatal mammalian cochlea (2000) Science, 288, pp. 2366-2368
  • Genzen, J.R., Van Cleve, W., Mc Gehee, D.S., Dorsal root ganglion neurons express multiple nicotinic acetylcholine receptor subtypes (2001) J. Neurophysiol., 86, pp. 1773-1782
  • Sato, K.Z., Fujii, T., Watanabe, Y., Yamada, S., Ando, T., Kazuko, F., Kawashima, K., Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines (1999) Neurosci. Lett., 266, pp. 17-20
  • Johnson, D.S., Martinez, J., Elgoyhen, A.B., Heinemann, S.F., McIntosh, J.M., α-conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: Preferential inhibition of homomeric α7 and α9 receptors (1995) Mol. Pharmacol., 48, pp. 194-199
  • Ellison, M., Gao, F., Wang, H.-L., Sine, S.M., Mcintosh, J.M., Olivera, B.M., α-conotoxins ImI and ImII target distinct regions of the human α7 nicotinic acetylcholine receptor and distinguish human nAChR subtypes (2004) Biochemistry, 43, pp. 16019-16026

Citas:

---------- APA ----------
Ellison, M., Haberlandt, C., Gomez-Casati, M.E., Watkins, M., Elgoyhen, A.B., McIntosh, J.M. & Olivera, B.M. (2006) . α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR. Biochemistry, 45(5), 1511-1517.
http://dx.doi.org/10.1021/bi0520129
---------- CHICAGO ----------
Ellison, M., Haberlandt, C., Gomez-Casati, M.E., Watkins, M., Elgoyhen, A.B., McIntosh, J.M., et al. "α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR" . Biochemistry 45, no. 5 (2006) : 1511-1517.
http://dx.doi.org/10.1021/bi0520129
---------- MLA ----------
Ellison, M., Haberlandt, C., Gomez-Casati, M.E., Watkins, M., Elgoyhen, A.B., McIntosh, J.M., et al. "α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR" . Biochemistry, vol. 45, no. 5, 2006, pp. 1511-1517.
http://dx.doi.org/10.1021/bi0520129
---------- VANCOUVER ----------
Ellison, M., Haberlandt, C., Gomez-Casati, M.E., Watkins, M., Elgoyhen, A.B., McIntosh, J.M., et al. α-RgIA: A novel conotoxin that specifically and potently blocks the α9α10 nAChR. Biochemistry. 2006;45(5):1511-1517.
http://dx.doi.org/10.1021/bi0520129