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Abstract:

Testicular, soluble adenylate cyclase has been purified by anion-exchange chromatography, gel filtration, and isoelectric focusing. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peak fractions from the latter purification step showed only one polypeptide band with an apparent molecular weight of about 69 000. The following hydrodynamic and molecular parameters have been established for this enzyme: sedimentation constant, 4.3 S; Stokes radius, 3.95 nm; partial specific volume, 0.74 mL.g-1; molecular weight, 74000; frictional ratio, 1.4. © 1981, American Chemical Society. All rights reserved.

Registro:

Documento: Artículo
Título:Manganese Ion Dependent Adenylate Cyclase Activity in Rat Testes: Purification and Properties
Autor:Kornblihtt, A.R.; Flawia, M.M.; Torres, H.N.
Filiación:Instituto de Investigaciones Bioquimicas, “Fundacion Campomar”, and Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:adenylate cyclase; manganese; animal; article; enzymology; isolation and purification; male; metabolism; molecular weight; protein conformation; rat; testis; Adenylate Cyclase; Animal; Male; Manganese; Molecular Weight; Protein Conformation; Rats; Support, Non-U.S. Gov't; Testis
Año:1981
Volumen:20
Número:5
Página de inicio:1262
Página de fin:1267
DOI: http://dx.doi.org/10.1021/bi00508a033
Título revista:Biochemistry
Título revista abreviado:Biochemistry
ISSN:00062960
CAS:adenylate cyclase, 9012-42-4; manganese, 16397-91-4, 7439-96-5; Adenylate Cyclase, EC 4.6.1.1; Manganese, 7439-96-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00062960_v20_n5_p1262_Kornblihtt

Referencias:

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Citas:

---------- APA ----------
Kornblihtt, A.R., Flawia, M.M. & Torres, H.N. (1981) . Manganese Ion Dependent Adenylate Cyclase Activity in Rat Testes: Purification and Properties. Biochemistry, 20(5), 1262-1267.
http://dx.doi.org/10.1021/bi00508a033
---------- CHICAGO ----------
Kornblihtt, A.R., Flawia, M.M., Torres, H.N. "Manganese Ion Dependent Adenylate Cyclase Activity in Rat Testes: Purification and Properties" . Biochemistry 20, no. 5 (1981) : 1262-1267.
http://dx.doi.org/10.1021/bi00508a033
---------- MLA ----------
Kornblihtt, A.R., Flawia, M.M., Torres, H.N. "Manganese Ion Dependent Adenylate Cyclase Activity in Rat Testes: Purification and Properties" . Biochemistry, vol. 20, no. 5, 1981, pp. 1262-1267.
http://dx.doi.org/10.1021/bi00508a033
---------- VANCOUVER ----------
Kornblihtt, A.R., Flawia, M.M., Torres, H.N. Manganese Ion Dependent Adenylate Cyclase Activity in Rat Testes: Purification and Properties. Biochemistry. 1981;20(5):1262-1267.
http://dx.doi.org/10.1021/bi00508a033