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Knowing that cell-surface receptors that recognize and respond to extracellular stimuli are key components for the regular communication between individual cells required for the survival of any living organism, the aim of the present work was to investigate the effect of H2R overexpression on the U937 signal transduction pathway and its consequences on cell proliferation and differentiation. The overexpression of H2R led to an increase in cAMP basal levels, a leftward shift of agonist concentration-response curves, and similar maximal response to agonist treatment, suggesting that overexpressed H2Rs act as functional spare receptors. In this system cells triggered several mechanisms tending to restore cAMP basal levels to those of the naïve cells. H2R overexpression induced PDE activity stimulation and GRK2 overexpression. In spite of the onset of these regulatory mechanisms, H2 agonist and rolipram treatments induced the terminal differentiation of the H2R overexpressed clone, conversely to the naïve cells. Present findings show that stably H2R overexpression alters cAMP signalling as the result of not only the amounts of second messenger generated but also the activation or upregulation of various components of signalling cascade, leading to an adapted biologically unique system. This adaptation may represent an advantage or a disadvantage, depending on the biological system, but in any case, the existence of compensatory mechanisms should be considered when a clinical treatment is designed. © 2006 Elsevier Inc. All rights reserved.


Documento: Artículo
Título:Histamine H2 receptor overexpression induces U937 cell differentiation despite triggered mechanisms to attenuate cAMP signalling
Autor:Monczor, F.; Fernandez, N.; Riveiro, E.; Mladovan, A.; Baldi, A.; Shayo, C.; Davio, C.
Filiación:Instituto de Biología Y Medicina Experimental, Buenos Aires, Argentina
Laboratorio de Radioisótopos, Facultad de Farmacia Y Bioquímica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina
Palabras clave:cAMP; Cell differentiation; GRK; Histamine receptors; PDE; Receptor overexpression; amthamine; cyclic AMP; G protein coupled receptor kinase 2; histamine H2 receptor; histamine H2 receptor agonist; phosphodiesterase; rolipram; article; cell differentiation; cell proliferation; concentration response; controlled study; desensitization; enzyme activity; gene overexpression; human; human cell; priority journal; signal transduction; Calcium; Cell Differentiation; Cyclic AMP; Dose-Response Relationship, Drug; Histamine Agonists; Humans; Phosphoric Diester Hydrolases; Radioligand Assay; Receptors, G-Protein-Coupled; Receptors, Histamine H2; Signal Transduction; U937 Cells
Página de inicio:1219
Página de fin:1228
Título revista:Biochemical Pharmacology
Título revista abreviado:Biochem. Pharmacol.
CAS:amthamine, 142437-67-0; cyclic AMP, 60-92-4; rolipram, 61413-54-5; Calcium, 7440-70-2; Cyclic AMP, 60-92-4; Histamine Agonists; Phosphoric Diester Hydrolases, EC 3.1.4.-; Receptors, G-Protein-Coupled; Receptors, Histamine H2


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---------- APA ----------
Monczor, F., Fernandez, N., Riveiro, E., Mladovan, A., Baldi, A., Shayo, C. & Davio, C. (2006) . Histamine H2 receptor overexpression induces U937 cell differentiation despite triggered mechanisms to attenuate cAMP signalling. Biochemical Pharmacology, 71(8), 1219-1228.
---------- CHICAGO ----------
Monczor, F., Fernandez, N., Riveiro, E., Mladovan, A., Baldi, A., Shayo, C., et al. "Histamine H2 receptor overexpression induces U937 cell differentiation despite triggered mechanisms to attenuate cAMP signalling" . Biochemical Pharmacology 71, no. 8 (2006) : 1219-1228.
---------- MLA ----------
Monczor, F., Fernandez, N., Riveiro, E., Mladovan, A., Baldi, A., Shayo, C., et al. "Histamine H2 receptor overexpression induces U937 cell differentiation despite triggered mechanisms to attenuate cAMP signalling" . Biochemical Pharmacology, vol. 71, no. 8, 2006, pp. 1219-1228.
---------- VANCOUVER ----------
Monczor, F., Fernandez, N., Riveiro, E., Mladovan, A., Baldi, A., Shayo, C., et al. Histamine H2 receptor overexpression induces U937 cell differentiation despite triggered mechanisms to attenuate cAMP signalling. Biochem. Pharmacol. 2006;71(8):1219-1228.