Abstract:
Polypeptide synthesis programmed by poly(U) and globin mRNA has been studied in cell-free extracts from wheat germ. A two-step reaction with a preincubation at high Mg++ levels followed by a second step carried out after a shift to a low Mg++ concentration and the addition of labeled amino acids is described. Under these conditions the initiation of polyphenylalanine synthesis can be blocked without affecting the elongation of polypeptide chains. This procedure allows the selective inhibition of polypeptide synthesis initiation without using any drug or antibiotic. © 1980, All rights reserved.
Registro:
Documento: |
Artículo
|
Título: | Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems |
Autor: | Algranati, I.D. |
Filiación: | Instituto de Investigaciones Bioquímicas “Fundación Campomar” Obligado 2490, Buenos Aires, 1428, Argentina Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
|
Palabras clave: | aurintricarboxylic acid; globin; magnesium; messenger RNA; polyuridylic acid; article; biosynthesis; cell free system; drug effect; kinetics; metabolism; peptide synthesis; plant; protein synthesis; wheat; Aurintricarboxylic Acid; Cell-Free System; Globins; Kinetics; Magnesium; Peptide Biosynthesis; Plants; Poly U; Protein Biosynthesis; RNA, Messenger; Triticum |
Año: | 1980
|
Volumen: | 96
|
Número: | 1
|
Página de inicio: | 54
|
Página de fin: | 60
|
DOI: |
http://dx.doi.org/10.1016/0006-291X(80)91180-8 |
Título revista: | Topics in Catalysis
|
Título revista abreviado: | Top. Catal.
|
ISSN: | 0006291x
|
CAS: | aurintricarboxylic acid, 4431-00-9; magnesium, 7439-95-4; polyuridylic acid, 27416-86-0; Aurintricarboxylic Acid, 4431-00-9; Globins, 9004-22-2; Magnesium, 7439-95-4; Poly U, 27416-86-0, 9004-22-2; RNA, Messenger
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291x_v96_n1_p54_Algranati |
Referencias:
- Schreier, M.H., Noll, H., (1971) Proc. Natl. Acad. Sci. U. S. A, 68, pp. 805-809. , second ed
- Haselkorn, R., Rothman-Denes, L.F., (1973) Ann. Rev. Biochem, 42, pp. 397-438
- Shafritz, D.A., Prichard, P.M., Gilbert, J.M., Anderson, W.F., (1970) Biochem. Biophys. Res. Commun, 38, pp. 721-727
- Marcus, A., Efron, D., Weeks, D.P., (1974) Methods in Enzymology, 30, pp. 749-754. , K. Moldave, L. Grossman, second ed
- Efron, D., Marcus, A., (1973) FEBS Lett, 33, pp. 23-27
- Revel, M., (1977) Molecular Mechanisms of Protein Biosynthesis, pp. 245-321. , H. Weissbach, S. Pestka, Academic Press, New York
- Weissbach, H., Ochoa, S., (1976) Ann. Rev. Biochem, 45, pp. 191-216
- Huang, M.T., Grollman, A.P., (1972) Mol. Pharmacol, 8, pp. 111-127
- Marcus, A., Bewley, J.D., Weeks, D.P., (1970) Science, 167, pp. 1735-1736
- Vázquez, D., (1979) Inhibitors of Protein Biosynthesis, pp. 28-30. , Springer Verlag, Berlin
- Lodish, H.F., Housman, D., Jacobsen, M., (1971) Biochemistry, 10, pp. 2348-2356
- Culp, W.J., McKeehan, W.L., Hardesty, B., (1969) Proc. Natl. Acad. Sci. U. S. A, 63, pp. 1431-1438. , second ed
Citas:
---------- APA ----------
(1980)
. Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems. Topics in Catalysis, 96(1), 54-60.
http://dx.doi.org/10.1016/0006-291X(80)91180-8---------- CHICAGO ----------
Algranati, I.D.
"Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems"
. Topics in Catalysis 96, no. 1
(1980) : 54-60.
http://dx.doi.org/10.1016/0006-291X(80)91180-8---------- MLA ----------
Algranati, I.D.
"Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems"
. Topics in Catalysis, vol. 96, no. 1, 1980, pp. 54-60.
http://dx.doi.org/10.1016/0006-291X(80)91180-8---------- VANCOUVER ----------
Algranati, I.D. Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems. Top. Catal. 1980;96(1):54-60.
http://dx.doi.org/10.1016/0006-291X(80)91180-8