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Abstract:

The microsomal glucosidases which act on the oligosaccharide that contains 2 N-acetylglucosamine, 9 mannose and 1-3 glucose residues have been studied. Two fractions were separated by differential solubility in detergent and phosphate solutions or by gel filtration. One of the fractions removed glucose from the oligosaccharide containing three glucose residues, and another fraction acted on the compounds containing one and two glucose residues. Both fractions were free of mannosidase. Some properties of the enzymes are described. © 1979.

Registro:

Documento: Artículo
Título:Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide
Autor:Ugalde, R.A.; Staneloni, R.J.; Leloir, L.F.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:glucosidase; animal experiment; dolichol diphosphate oligosaccharide; in vitro study; liver; microsome; rat; Animal; Glucosidases; Kinetics; Microsomes, Liver; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Rats; Substrate Specificity
Año:1979
Volumen:91
Número:3
Página de inicio:1174
Página de fin:1181
DOI: http://dx.doi.org/10.1016/0006-291X(79)92003-5
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:glucosidase, 9033-06-1; Glucosidases, EC 3.2.1.-; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v91_n3_p1174_Ugalde

Referencias:

  • Staneloni, Leloir, The biosynthetic pathway of the asparagine-linked oligosaccharides of glycoproteins (1978) Trends in Biochemical Sciences, 4, pp. 65-67
  • Parodi, Leloir, (1979) Biochim. Biophys. Acta, 559, pp. 1-37
  • Ugalde, Staneloni, Leloir, (1978) FEBS Lett, 91, pp. 209-212
  • parodi, Staneloni, Cantarella, Leloir, Behrens, Carminatti, Levy, (1973) Carbohyd. Research, 26, pp. 393-400
  • Staneloni, Leloir, (1978) Proc. Natl. Acad. Sci. U. S. A, 75, pp. 1162-1166
  • Spiro, Spiro, Bhoyroo, (1976) J. Biol. Chem, 251, pp. 6400-6408
  • Tabas, Schlesinger, Kornfeld, (1978) J. Biol. Chem, 253, pp. 716-722
  • Robbins, Hubbard, Turco, Wirth, (1977) Cell, 12, pp. 893-900
  • Herscovics, Bugge, Jeanloz, (1977) J. Biol. Chem, 222, pp. 2271-2277
  • Scher, Waechter, (1979) J. Biol. Chem, 254, pp. 2630-2637

Citas:

---------- APA ----------
Ugalde, R.A., Staneloni, R.J. & Leloir, L.F. (1979) . Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide. Biochemical and Biophysical Research Communications, 91(3), 1174-1181.
http://dx.doi.org/10.1016/0006-291X(79)92003-5
---------- CHICAGO ----------
Ugalde, R.A., Staneloni, R.J., Leloir, L.F. "Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide" . Biochemical and Biophysical Research Communications 91, no. 3 (1979) : 1174-1181.
http://dx.doi.org/10.1016/0006-291X(79)92003-5
---------- MLA ----------
Ugalde, R.A., Staneloni, R.J., Leloir, L.F. "Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide" . Biochemical and Biophysical Research Communications, vol. 91, no. 3, 1979, pp. 1174-1181.
http://dx.doi.org/10.1016/0006-291X(79)92003-5
---------- VANCOUVER ----------
Ugalde, R.A., Staneloni, R.J., Leloir, L.F. Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide. Biochem. Biophys. Res. Commun. 1979;91(3):1174-1181.
http://dx.doi.org/10.1016/0006-291X(79)92003-5