Crude preparations of cyclic adenosine 3′, 5′-monophosphate phosphodiesterase were activated 1.5 to 2 fold by incubation with ATP, Mg2+ and cyclic AMP in a reaction which was both, time and temperature dependent. Cyclic AMP phosphodiesterase remained in an activated state upon filtration of the enzymatic preparation through Sephadex G-25 and ion-exchange chromatography. Activation of the enzyme in the presence of [γ 32P]ATP resulted in a significant amount of [32P] protein-bound radioactivity. Reversible deactivation of cyclic AMP phosphodiesterase was enhanced by Mg2+ and was accompanied by the release of [32P] protein bound radioactivity. The evidence is consistent with a mechanism for controlling cyclic AMP phosphodiesterase through phosphorylation-dephosphorylation sequence. © 1979.
Documento: | Artículo |
Título: | Cyclic adenosine 3′, 5′-monophosphate phosphodiesterase from Mucor rouxii: Regulation of enzyme activity by phosphorylation and dephosphorylation |
Autor: | Galvagno, M.A.; Moreno, S.; Cantore, M.L.; Passeron, S. |
Filiación: | Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
Palabras clave: | 3',5' cyclic nucleotide phosphodiesterase; magnesium; article; enzyme activation; enzymology; isolation and purification; kinetics; metabolism; Mucor; phosphorylation; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Enzyme Activation; Kinetics; Magnesium; Mucor; Phosphorylation |
Año: | 1979 |
Volumen: | 89 |
Número: | 3 |
Página de inicio: | 779 |
Página de fin: | 785 |
DOI: | http://dx.doi.org/10.1016/0006-291X(79)91846-1 |
Título revista: | Biochemical and Biophysical Research Communications |
Título revista abreviado: | Biochem. Biophys. Res. Commun. |
ISSN: | 0006291X |
CODEN: | BBRCA |
CAS: | 3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; magnesium, 7439-95-4; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Magnesium, 7439-95-4 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v89_n3_p779_Galvagno |