Abstract:
Insect extracts were found to contain a lipid which becomes glycosylated when incubated with uridine diphosphate glucose or uridine diphosphate N-acetylglucosamine and microsomal enzymes of rat liver. The behaviour of the lipid on column or thin-layer chromatography and its stability to acid were equal to those of dolichol monophosphate. The glycosylated compounds were acid labile. Treatment with alkali of the acetylglucosaminyl compound produced a substance that migrated like a hexose phosphate on electrophoresis and that liberated acetylglucosamine on treatment with alkaline phosphatase. The behaviour of the insect glucosylated lipid on thin-layer chromatography and its stability to phenol were similar to dolichol monophosphate glucose and different from ficaprenyl monophosphate glucose. It is concluded that the insect glycosyl acceptor lipid is an α saturated polyprenyl phosphate. © 1975.
Registro:
Documento: |
Artículo
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Título: | Glycosyl transfer to an acceptor lipid from insects |
Autor: | Quesada Allué, L.A.; Belocopitow, E.; Maréchal, L.R. |
Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina
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Palabras clave: | lipid; uridine diphosphate glucose; uridine diphosphate n acetylglucosamine; arthropod; in vitro study; liver; microsome; rat; theoretical study; Acetylglucosamine; Animal; Chromatography, Thin Layer; Diptera; Glucose; Glycolipids; Insects; Phospholipids; Rats; Species Specificity |
Año: | 1975
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Volumen: | 66
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Número: | 4
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Página de inicio: | 1201
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Página de fin: | 1208
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DOI: |
http://dx.doi.org/10.1016/0006-291X(75)90486-6 |
Título revista: | Biochemical and Biophysical Research Communications
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Título revista abreviado: | Biochem. Biophys. Res. Commun.
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ISSN: | 0006291X
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CODEN: | BBRCA
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CAS: | lipid, 66455-18-3; uridine diphosphate glucose, 133-89-1; uridine diphosphate n acetylglucosamine, 528-04-1; Acetylglucosamine, 7512-17-6; Glucose, 50-99-7; Glycolipids; Phospholipids
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v66_n4_p1201_QuesadaAllue |
Referencias:
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Citas:
---------- APA ----------
Quesada Allué, L.A., Belocopitow, E. & Maréchal, L.R.
(1975)
. Glycosyl transfer to an acceptor lipid from insects. Biochemical and Biophysical Research Communications, 66(4), 1201-1208.
http://dx.doi.org/10.1016/0006-291X(75)90486-6---------- CHICAGO ----------
Quesada Allué, L.A., Belocopitow, E., Maréchal, L.R.
"Glycosyl transfer to an acceptor lipid from insects"
. Biochemical and Biophysical Research Communications 66, no. 4
(1975) : 1201-1208.
http://dx.doi.org/10.1016/0006-291X(75)90486-6---------- MLA ----------
Quesada Allué, L.A., Belocopitow, E., Maréchal, L.R.
"Glycosyl transfer to an acceptor lipid from insects"
. Biochemical and Biophysical Research Communications, vol. 66, no. 4, 1975, pp. 1201-1208.
http://dx.doi.org/10.1016/0006-291X(75)90486-6---------- VANCOUVER ----------
Quesada Allué, L.A., Belocopitow, E., Maréchal, L.R. Glycosyl transfer to an acceptor lipid from insects. Biochem. Biophys. Res. Commun. 1975;66(4):1201-1208.
http://dx.doi.org/10.1016/0006-291X(75)90486-6