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Abstract:

The presence and some properties of DNA polymerases isolated from normal human lymphocytes, non stimulated and stimulated by phytohemagglutinin, are described. In the non stimulated lymphocytes two cytoplasmic DNA polymerases are found, one eluting from DEAE cellulose at 0.07 M NaCl (CIn) and the other at 0.13 M NaCl (CIIn). In the nuclear soluble fraction only one enzyme activity is found (NIn) which does not adsorb to DEAE cellulose. In the cytoplasm of stimulated lymphocytes only one enzyme activity is detected (CIs) which elutes from DEAE cellulose at 0.12 M NaCl. The nuclear soluble fraction contains two activities, NIs, which does not adsorb to DEAE cellulose, and NIIs, which elutes from DEAE cellulose at 0.07 M NaCl. Some properties of the different enzymes are described which indicate that NIn and NIs enzymes are clearly different from the others. © 1974.

Registro:

Documento: Artículo
Título:DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes
Autor:Fridlender, B.; Virasoro, S.; Blau, S.; Mordoh, J.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires (28), Argentina
Palabras clave:1,10 phenanthroline; bovine serum albumin; dithiothreitol; dna; dna polymerase; enzyme; magnesium chloride; manganese; phytohemagglutinin; potassium chloride; cytoplasm; deoxynucleoside triphosphate; human; in vitro study; lymphocyte; lymphocyte transformation; theoretical study; thymus; Animal; Cattle; Cell Nucleus; Chromatography, DEAE-Cellulose; Cytoplasm; Dithiothreitol; DNA Nucleotidyltransferases; Human; Hydrogen-Ion Concentration; Lectins; Lymphocyte Activation; Lymphocytes; Magnesium; Manganese; Phenanthrolines; Serum Albumin, Bovine; Sodium Chloride; Stimulation, Chemical; Tritium
Año:1974
Volumen:60
Número:3
Página de inicio:983
Página de fin:990
DOI: http://dx.doi.org/10.1016/0006-291X(74)90410-0
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:1,10 phenanthroline, 3829-86-5, 66-71-7; dithiothreitol, 3483-12-3; DNA polymerase, 37217-33-7; DNA, 9007-49-2; magnesium chloride, 7786-30-3, 7791-18-6; manganese, 16397-91-4, 7439-96-5; phytohemagglutinin, 9008-97-3; potassium chloride, 7447-40-7; Dithiothreitol, 3483-12-3; DNA Nucleotidyltransferases, EC 2.7.7.-; Lectins; Magnesium, 7439-95-4; Manganese, 7439-96-5; Phenanthrolines; Serum Albumin, Bovine; Sodium Chloride, 7647-14-5; Tritium, 10028-17-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v60_n3_p983_Fridlender

Referencias:

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Citas:

---------- APA ----------
Fridlender, B., Virasoro, S., Blau, S. & Mordoh, J. (1974) . DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes. Biochemical and Biophysical Research Communications, 60(3), 983-990.
http://dx.doi.org/10.1016/0006-291X(74)90410-0
---------- CHICAGO ----------
Fridlender, B., Virasoro, S., Blau, S., Mordoh, J. "DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes" . Biochemical and Biophysical Research Communications 60, no. 3 (1974) : 983-990.
http://dx.doi.org/10.1016/0006-291X(74)90410-0
---------- MLA ----------
Fridlender, B., Virasoro, S., Blau, S., Mordoh, J. "DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes" . Biochemical and Biophysical Research Communications, vol. 60, no. 3, 1974, pp. 983-990.
http://dx.doi.org/10.1016/0006-291X(74)90410-0
---------- VANCOUVER ----------
Fridlender, B., Virasoro, S., Blau, S., Mordoh, J. DNA polymerases from non stimulated and phytohemagglutinin stimulated normal human lymphocytes. Biochem. Biophys. Res. Commun. 1974;60(3):983-990.
http://dx.doi.org/10.1016/0006-291X(74)90410-0