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Abstract:

Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosyl-(1rarr4)-2-acetamido-2-deoxy-D-glucose). The formation of the disaccharide containing lipid was increased by incubation with crude liver lipids or by reincubation with unlabelled UDP-N-acetylglucosamine. The labelling in the latter compound varied according to whether one of the N-acetylglucosamyl residues arose from a crude lipid or from unlabelled UDP-N-acetylglucosamine. Evidence is presented indicating that the compounds are dolichol pyrophosphate derivatives. © 1973.

Registro:

Documento: Artículo
Título:The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
Autor:Leloir, L.F.; Staneloni, R.J.; Carminatti, H.; Behrens, N.H.
Filiación:Instituto de Investigaciones Bioquímicas, Fundación Campomar, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires (28), Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado, 2490 Buenos Aires (28), Argentina
Palabras clave:acetic acid derivative; borane derivative; carbon; disaccharide; fatty alcohol; glucosamine; lipid; magnesium; terpene; uridine diphosphate sugar; animal; article; biosynthesis; drug effect; hydrolysis; ion exchange chromatography; liver microsome; metabolism; oxidation reduction reaction; paper chromatography; paper electrophoresis; rat; Acetic Acids; Animal; Borohydrides; Carbon Isotopes; Chromatography, DEAE-Cellulose; Chromatography, Paper; Disaccharides; Electrophoresis, Paper; Fatty Alcohols; Glucosamine; Hydrolysis; Lipids; Magnesium; Microsomes, Liver; Oxidation-Reduction; Rats; Terpenes; Uridine Diphosphate Sugars
Año:1973
Volumen:52
Número:4
Página de inicio:1285
Página de fin:1292
DOI: http://dx.doi.org/10.1016/0006-291X(73)90640-2
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:carbon, 7440-44-0; glucosamine, 3416-24-8, 4607-22-1; lipid, 66455-18-3; magnesium, 7439-95-4; Acetic Acids; Borohydrides; Carbon Isotopes; Disaccharides; Fatty Alcohols; Glucosamine, 3416-24-8; Lipids; Magnesium, 7439-95-4; Terpenes; Uridine Diphosphate Sugars
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v52_n4_p1285_Leloir

Referencias:

  • Tetas, Chao, Molnar, (1970) Arch. Biochem. Biophys, 138, p. 135
  • Molnar, Chao, Ikehara, Phosphoryl-n-acetylglucosamine transfer to a lipid acceptor of liver microsomal preparations (1971) Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 239, p. 40
  • Behrens, Parodi, Leloir, Krisman, (1971) Arch. Biochem. Biophys, 143, p. 375
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  • Leseney, Bourrillon, Kornfeld, (1972) Arch. Biochem. Biophys, 153, p. 831
  • Parodi, Behrens, Leloir, Carminatti, (1972) Proc. Nat. Acad. Sci. US, 69, p. 3268
  • Parodi, A.J., Staneloni, R.J., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H. and Levy, J.A., Carbohyd. Res. (in press)

Citas:

---------- APA ----------
Leloir, L.F., Staneloni, R.J., Carminatti, H. & Behrens, N.H. (1973) . The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative. Biochemical and Biophysical Research Communications, 52(4), 1285-1292.
http://dx.doi.org/10.1016/0006-291X(73)90640-2
---------- CHICAGO ----------
Leloir, L.F., Staneloni, R.J., Carminatti, H., Behrens, N.H. "The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative" . Biochemical and Biophysical Research Communications 52, no. 4 (1973) : 1285-1292.
http://dx.doi.org/10.1016/0006-291X(73)90640-2
---------- MLA ----------
Leloir, L.F., Staneloni, R.J., Carminatti, H., Behrens, N.H. "The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative" . Biochemical and Biophysical Research Communications, vol. 52, no. 4, 1973, pp. 1285-1292.
http://dx.doi.org/10.1016/0006-291X(73)90640-2
---------- VANCOUVER ----------
Leloir, L.F., Staneloni, R.J., Carminatti, H., Behrens, N.H. The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative. Biochem. Biophys. Res. Commun. 1973;52(4):1285-1292.
http://dx.doi.org/10.1016/0006-291X(73)90640-2