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Solari, C.; Echegaray, C.V.; Luzzani, C.; Cosentino, M.S.; Waisman, A.; Petrone, M.V.; Francia, M.; Sassone, A.; Canizo, J.; Sevlever, G.; Barañao, L.; Miriuka, S.; Guberman, A. "Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2" (2016) Biochemical and Biophysical Research Communications. 473(1):194-199
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Abstract:

Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neurogenesis. In this work, we found that Prmt8 is expressed in mouse embryonic stem cells (ESC) and in induced pluripotent stem cells, and modulated along differentiation to neural precursor cells. We found that Prmt8 promoter activity is induced by the pluripotency transcription factors Oct4, Sox2 and Nanog. Moreover, endogenous Prmt8 mRNA levels were reduced in ESC transfected with Sox2 shRNA vector. As a whole, our results indicate that Prmt8 is expressed in pluripotent stem cells and its transcription is modulated by pluripotency transcription factors. These findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells. © 2016 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2
Autor:Solari, C.; Echegaray, C.V.; Luzzani, C.; Cosentino, M.S.; Waisman, A.; Petrone, M.V.; Francia, M.; Sassone, A.; Canizo, J.; Sevlever, G.; Barañao, L.; Miriuka, S.; Guberman, A.
Filiación:Laboratorio de Regulación Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, Buenos Aires, C1428EGA, Argentina
Instituto de Química Biológica - Ciencias Exactas y Naturales (IQUIBICEN), UBA/CONICET, Buenos Aires, Argentina
Laboratorio de Investigación Aplicada A Las Neurociencias (LIAN)-CONICET, Fundación Para la Lucha Contra Las Enfermedades Neurológicas de la Infancia (FLENI), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
4 Ruta 9 Km. 52.5, Escobar, Buenos Aires, B1625XAF, Argentina
Palabras clave:Embryonic stem cells; Induced pluripotent stem cells; Nanog; Oct4; Prmt8; Sox2; messenger RNA; octamer transcription factor 4; protein arginine methyltransferase; protein arginine methyltransferase 8; short hairpin RNA; transcription factor NANOG; transcription factor Sox2; unclassified drug; homeodomain protein; Nanog protein, mouse; octamer transcription factor 4; Pou5f1 protein, mouse; PRMT8 protein, mouse; protein arginine methyltransferase; small interfering RNA; Sox2 protein, mouse; transcription factor Sox; animal cell; Article; binding site; cell differentiation; controlled study; down regulation; embryo; gene expression; genetic transfection; mouse; mouse embryonic stem cell; neural stem cell; nonhuman; nuclear reprogramming; pluripotent stem cell; priority journal; promoter region; upregulation; 3T3 cell line; animal; cell differentiation; cytology; fibroblast; gene expression regulation; metabolism; nerve cell; pluripotent stem cell; reverse transcription polymerase chain reaction; Animals; Cell Differentiation; Down-Regulation; Fibroblasts; Gene Expression Regulation, Enzymologic; Homeodomain Proteins; Mice; Neurons; NIH 3T3 Cells; Octamer Transcription Factor-3; Pluripotent Stem Cells; Promoter Regions, Genetic; Protein-Arginine N-Methyltransferases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Small Interfering; SOXB1 Transcription Factors; Transfection
Año:2016
Volumen:473
Número:1
Página de inicio:194
Página de fin:199
DOI: http://dx.doi.org/10.1016/j.bbrc.2016.03.077
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:protein arginine methyltransferase; Homeodomain Proteins; Nanog protein, mouse; Octamer Transcription Factor-3; Pou5f1 protein, mouse; PRMT8 protein, mouse; Protein-Arginine N-Methyltransferases; RNA, Small Interfering; Sox2 protein, mouse; SOXB1 Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v473_n1_p194_Solari

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Citas:

---------- APA ----------
Solari, C., Echegaray, C.V., Luzzani, C., Cosentino, M.S., Waisman, A., Petrone, M.V., Francia, M.,..., Guberman, A. (2016) . Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2. Biochemical and Biophysical Research Communications, 473(1), 194-199.
http://dx.doi.org/10.1016/j.bbrc.2016.03.077
---------- CHICAGO ----------
Solari, C., Echegaray, C.V., Luzzani, C., Cosentino, M.S., Waisman, A., Petrone, M.V., et al. "Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2" . Biochemical and Biophysical Research Communications 473, no. 1 (2016) : 194-199.
http://dx.doi.org/10.1016/j.bbrc.2016.03.077
---------- MLA ----------
Solari, C., Echegaray, C.V., Luzzani, C., Cosentino, M.S., Waisman, A., Petrone, M.V., et al. "Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2" . Biochemical and Biophysical Research Communications, vol. 473, no. 1, 2016, pp. 194-199.
http://dx.doi.org/10.1016/j.bbrc.2016.03.077
---------- VANCOUVER ----------
Solari, C., Echegaray, C.V., Luzzani, C., Cosentino, M.S., Waisman, A., Petrone, M.V., et al. Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2. Biochem. Biophys. Res. Commun. 2016;473(1):194-199.
http://dx.doi.org/10.1016/j.bbrc.2016.03.077