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Abstract:

In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc.

Registro:

Documento: Artículo
Título:Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation
Autor:Ayala-Peña, V.; Santillán, G.; Scolaro, L.
Filiación:Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional Del sur, San Juan 670, (B8000ICN), Bahía Blanca, Argentina
Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Pabellón 2, Piso 4, Buenos Aires, 1428, Argentina
Palabras clave:Bone morphogenetic proteins; Differentiation; Measles virus; Osteoblasts; Otosclerosis; 2 morpholino 8 phenylchromone; adenosine triphosphate; alkaline phosphatase; bone morphogenetic protein 4; bone morphogenetic protein 5; bone sialo protein; messenger RNA; osteogenic protein 1; phosphatidylinositol 3 kinase; procollagen C proteinase; protein; protein kinase B; unclassified drug; 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; adenosine 5' o (3 thiotriphosphate); adenosine 5'-O-(3-thiotriphosphate); adenosine triphosphate; alkaline phosphatase; bone morphogenetic protein; bone morphogenetic protein 4; chromone derivative; drug derivative; morpholine derivative; phosphatidylinositol 3 kinase; animal cell; animal experiment; animal model; Article; bone development; cell differentiation; controlled study; experimental infection; in vitro study; measles; Measles virus; nonhuman; osteoblast; protein expression; rat; stimulation; virus load; animal; article; biosynthesis; bone; cell culture; differentiation; drug antagonism; drug effect; metabolism; otosclerosis; physiology; virology; virus replication; Bone morphogenetic proteins; Differentiation; Measles virus; Osteoblasts; Otosclerosis; Adenosine Triphosphate; Alkaline Phosphatase; Animals; Bone and Bones; Bone Morphogenetic Protein 4; Cell Differentiation; Cells, Cultured; Chromones; Measles virus; Morpholines; Osteoblasts; Osteogenesis; Otosclerosis; Phosphatidylinositol 3-Kinases; Rats; Virus Replication
Año:2014
Volumen:451
Número:4
Página de inicio:609
Página de fin:614
DOI: http://dx.doi.org/10.1016/j.bbrc.2014.08.027
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:2 morpholino 8 phenylchromone, 154447-36-6; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; alkaline phosphatase, 9001-78-9; phosphatidylinositol 3 kinase, 115926-52-8; procollagen C proteinase, 68651-95-6; protein, 67254-75-5; protein kinase B, 148640-14-6; adenosine 5' o (3 thiotriphosphate), 35094-46-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v451_n4_p609_AyalaPena

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Citas:

---------- APA ----------
Ayala-Peña, V., Santillán, G. & Scolaro, L. (2014) . Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation. Biochemical and Biophysical Research Communications, 451(4), 609-614.
http://dx.doi.org/10.1016/j.bbrc.2014.08.027
---------- CHICAGO ----------
Ayala-Peña, V., Santillán, G., Scolaro, L. "Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation" . Biochemical and Biophysical Research Communications 451, no. 4 (2014) : 609-614.
http://dx.doi.org/10.1016/j.bbrc.2014.08.027
---------- MLA ----------
Ayala-Peña, V., Santillán, G., Scolaro, L. "Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation" . Biochemical and Biophysical Research Communications, vol. 451, no. 4, 2014, pp. 609-614.
http://dx.doi.org/10.1016/j.bbrc.2014.08.027
---------- VANCOUVER ----------
Ayala-Peña, V., Santillán, G., Scolaro, L. Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation. Biochem. Biophys. Res. Commun. 2014;451(4):609-614.
http://dx.doi.org/10.1016/j.bbrc.2014.08.027