Artículo

Dávola, M.E.; Alonso, F.; Cabrera, G.M.; Ramírez, J.A.; Barquero, A.A. "Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins" (2012) Biochemical and Biophysical Research Communications. 427(1):107-112
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Abstract:

The need to develop novel antiviral agents encouraged us to assess the antiviral activity of synthetic sterol analogues with a diamide side chains. Cytotoxicity and antiviral activity of a family of azasterol previously synthesized was evaluated against herpes simplex virus 1 (HSV-1) (KOS and B2006) and vesicular stomatitis virus (VSV). This family of compounds was extended by the synthesis of novel analogs using an Ugi multicomponent reaction and their ability to inhibit viral multiplication was also evaluated. The results show that some of the compounds tested exert an antiviral activity. Besides, the effect of the azasterols on the intracellular localization of viral glycoproteins was examined. Strikingly, alteration on the glycoprotein D (gD) of HSV-1 fluorescence pattern was observed with both the antiherpetic compounds and the inactive azasterols. © 2012 Elsevier Inc.

Registro:

Documento: Artículo
Título:Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins
Autor:Dávola, M.E.; Alonso, F.; Cabrera, G.M.; Ramírez, J.A.; Barquero, A.A.
Filiación:Laboratorio de Virologia, Departamento de Quimica Biologica and IQUIBICEN (CONICET-Facultad de Ciencias Exactas y Naturales), Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, C1428EGA Buenos Aires, Argentina
Departamento de Quimica Organica and UMYMFOR (CONICET-Facultad de Ciencias Exactas y Naturales), Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 3, C1428EGA Buenos Aires, Argentina
Palabras clave:Antiviral; Azasterol; Glycoprotein transport; HSV-1; Ugi reaction; VSV; azacosterol; diamide; glycoprotein D; n [(tert butylcarbamoyl)methyl] n (2 chlorophenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (2 ethyl 6 methylphenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (2 fluorophenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (3 trifluorophenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (3,4 dichlorophenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (4 fluorophenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (4 methoxyphenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; n [(tert butylcarbamoyl)methyl] n (4 methylphenyl) 3beta hydroxyandrost 5 ene 17beta carboxamide; unclassified drug; virus glycoprotein; animal cell; antiviral activity; article; chemical reaction; drug synthesis; fluorescence analysis; Herpes simplex virus 1; nonhuman; priority journal; protein localization; virogenesis; virus inhibition; Antiviral Agents; Diamide; Herpesvirus 1, Human; Humans; Intracellular Space; Sterols; Viral Envelope Proteins; Virus Replication; Human herpesvirus 1; Vesicular stomatitis virus
Año:2012
Volumen:427
Número:1
Página de inicio:107
Página de fin:112
DOI: http://dx.doi.org/10.1016/j.bbrc.2012.09.019
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:azacosterol, 1249-84-9, 313-05-3; diamide, 10465-78-8; Antiviral Agents; Diamide, 10465-78-8; Sterols; Viral Envelope Proteins; glycoprotein D, Human herpesvirus 1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v427_n1_p107_Davola

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Citas:

---------- APA ----------
Dávola, M.E., Alonso, F., Cabrera, G.M., Ramírez, J.A. & Barquero, A.A. (2012) . Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins. Biochemical and Biophysical Research Communications, 427(1), 107-112.
http://dx.doi.org/10.1016/j.bbrc.2012.09.019
---------- CHICAGO ----------
Dávola, M.E., Alonso, F., Cabrera, G.M., Ramírez, J.A., Barquero, A.A. "Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins" . Biochemical and Biophysical Research Communications 427, no. 1 (2012) : 107-112.
http://dx.doi.org/10.1016/j.bbrc.2012.09.019
---------- MLA ----------
Dávola, M.E., Alonso, F., Cabrera, G.M., Ramírez, J.A., Barquero, A.A. "Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins" . Biochemical and Biophysical Research Communications, vol. 427, no. 1, 2012, pp. 107-112.
http://dx.doi.org/10.1016/j.bbrc.2012.09.019
---------- VANCOUVER ----------
Dávola, M.E., Alonso, F., Cabrera, G.M., Ramírez, J.A., Barquero, A.A. Sterol analogues with diamide side chains interfere with the intracellular localization of viral glycoproteins. Biochem. Biophys. Res. Commun. 2012;427(1):107-112.
http://dx.doi.org/10.1016/j.bbrc.2012.09.019