Artículo

Martínez, M.G.; Prado Acosta, M.; Candurra, N.A.; Ruzal, S.M. "S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection" (2012) Biochemical and Biophysical Research Communications. 422(4):590-595
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Abstract:

It has been previously described that S-layer binds to the C-type lectin DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN, CD209). It was also shown that DC-SIGN is a cell-surface adhesion factor that enhances viral entry of several virus families. Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. However no inhibition effect was observed in 3T3 wild type cells or in 3T3 cells expressing liver/lymph node-specific ICAM-3 grabbing nonintegrin (L-SIGN or DC-SIGNR or CD209L).Treatments with S-layer during different times in the infection demonstrated that inhibition was only observed when S-layer was presented in early stages of the viral infection. This inhibition does not involve the classic recognition of mannose by this C-type lectin as the S-layer showed no evidence to be glycosylated. In fact, the highly basic nature of the S-layer (pI>9.5) seems to be involved in electrostatic interactions between DC-SIGN and S-layer, since high pH abolished the inhibitory effect on infection cause by the S-layer. In silico analysis predicts a Ca 2+-dependant carbohydrate recognition domain in the SlpA protein.This novel characteristic of the S-layer, a GRAS status protein, contribute to the pathogen exclusion reported for this probiotic strain and may be applied as an antiviral agent to inhibit several kinds of viruses. © 2012 Elsevier Inc..

Registro:

Documento: Artículo
Título:S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection
Autor:Martínez, M.G.; Prado Acosta, M.; Candurra, N.A.; Ruzal, S.M.
Filiación:Departamento de Química Biológica, Facultad Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, Ciudad Universitaria Pabellon II. 4 piso, C1428EGA Buenos Aires, Argentina
Palabras clave:Antiviral; Lactobacillus; Surface layer; antivirus agent; calcium ion; CD209 antigen; intercellular adhesion molecule 3; probiotic agent; protein slpa; unclassified drug; animal cell; Argentine hemorrhagic fever; article; cell strain 3T3; computer model; disease course; evaluation; human; Lactobacillus acidophilus; nonhuman; priority journal; virus entry; 3T3 Cells; Amino Acid Sequence; Animals; Antiviral Agents; Arenaviridae Infections; Bacterial Proteins; Cell Adhesion Molecules; Cercopithecus aethiops; Consensus Sequence; Junin virus; Lactobacillus acidophilus; Lectins, C-Type; Membrane Glycoproteins; Mice; Molecular Sequence Data; Receptors, Cell Surface; Vero Cells; Virus Internalization; Junin virus; Lactobacillus; Lactobacillus acidophilus
Año:2012
Volumen:422
Número:4
Página de inicio:590
Página de fin:595
DOI: http://dx.doi.org/10.1016/j.bbrc.2012.05.031
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:calcium ion, 14127-61-8; Antiviral Agents; Bacterial Proteins; Cell Adhesion Molecules; DC-specific ICAM-3 grabbing nonintegrin; Lectins, C-Type; Membrane Glycoproteins; Receptors, Cell Surface; S-layer proteins; surface layer protein A, Bacteria
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v422_n4_p590_Martinez

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Citas:

---------- APA ----------
Martínez, M.G., Prado Acosta, M., Candurra, N.A. & Ruzal, S.M. (2012) . S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection. Biochemical and Biophysical Research Communications, 422(4), 590-595.
http://dx.doi.org/10.1016/j.bbrc.2012.05.031
---------- CHICAGO ----------
Martínez, M.G., Prado Acosta, M., Candurra, N.A., Ruzal, S.M. "S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection" . Biochemical and Biophysical Research Communications 422, no. 4 (2012) : 590-595.
http://dx.doi.org/10.1016/j.bbrc.2012.05.031
---------- MLA ----------
Martínez, M.G., Prado Acosta, M., Candurra, N.A., Ruzal, S.M. "S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection" . Biochemical and Biophysical Research Communications, vol. 422, no. 4, 2012, pp. 590-595.
http://dx.doi.org/10.1016/j.bbrc.2012.05.031
---------- VANCOUVER ----------
Martínez, M.G., Prado Acosta, M., Candurra, N.A., Ruzal, S.M. S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection. Biochem. Biophys. Res. Commun. 2012;422(4):590-595.
http://dx.doi.org/10.1016/j.bbrc.2012.05.031