Artículo

Solari, C.; Losino, N.; Luzzani, C.; Waisman, A.; Bluguermann, C.; Questa, M.; Sevlever, G.; Miriuka, S.; Barañao, L.; Guberman, A. "Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium" (2011) Biochemical and Biophysical Research Communications. 410(2):252-257
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Abstract:

Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc.

Registro:

Documento: Artículo
Título:Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
Autor:Solari, C.; Losino, N.; Luzzani, C.; Waisman, A.; Bluguermann, C.; Questa, M.; Sevlever, G.; Miriuka, S.; Barañao, L.; Guberman, A.
Filiación:Laboratorio de Regulación de la Expresión Génica en el Crecimiento, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Biología del Desarrollo Celular, FLENI, Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:BGC; BGC-CM; CM; Conditioned medium; Culture conditions; ESC; GC; Induced pluripotent stem cell; IPSC; LIF; MEF; MESC; Pluripotency; Self-renewal; SM; animal cell; article; cell differentiation; cell type; controlled study; fibroblast; gene expression; granulosa cell; immunofluorescence; in vitro study; in vivo study; Lentivirinae; marker gene; mouse; nonhuman; pluripotent stem cell; priority journal; Animals; Cattle; Cell Culture Techniques; Cell Line; Culture Media, Conditioned; Female; Granulosa Cells; Induced Pluripotent Stem Cells; Mice; Octamer Transcription Factor-3; Regeneration; Bovinae
Año:2011
Volumen:410
Número:2
Página de inicio:252
Página de fin:257
DOI: http://dx.doi.org/10.1016/j.bbrc.2011.05.126
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:Culture Media, Conditioned; Octamer Transcription Factor-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n2_p252_Solari

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Citas:

---------- APA ----------
Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., Sevlever, G.,..., Guberman, A. (2011) . Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium. Biochemical and Biophysical Research Communications, 410(2), 252-257.
http://dx.doi.org/10.1016/j.bbrc.2011.05.126
---------- CHICAGO ----------
Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., et al. "Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium" . Biochemical and Biophysical Research Communications 410, no. 2 (2011) : 252-257.
http://dx.doi.org/10.1016/j.bbrc.2011.05.126
---------- MLA ----------
Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., et al. "Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium" . Biochemical and Biophysical Research Communications, vol. 410, no. 2, 2011, pp. 252-257.
http://dx.doi.org/10.1016/j.bbrc.2011.05.126
---------- VANCOUVER ----------
Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., et al. Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium. Biochem. Biophys. Res. Commun. 2011;410(2):252-257.
http://dx.doi.org/10.1016/j.bbrc.2011.05.126