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Abstract:

In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3′ end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level. © 2009 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30
Autor:Bercovich, N.; Levin, M.J.; Vazquez, M.P.
Filiación:INGEBI-CONICET, Vta. de Obligado 2490, 1428 Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Buenos Aires, Argentina
Palabras clave:Drug target; mRNA processing; Polyadenylation factors; Protein interactions; Trypanosome diseases; amino acid; cleavage and polyadenylation specificity factor; cpsf30 protein; fip1 protein; nuclear protein; protein; unclassified drug; amino acid blood level; article; cell surface; controlled study; human; human cell; nonhuman; parasite viability; polyadenylation; priority journal; protein interaction; Trypanosoma; Amino Acid Sequence; Animals; Cleavage And Polyadenylation Specificity Factor; Molecular Sequence Data; mRNA Cleavage and Polyadenylation Factors; Polyadenylation; Trypanosoma brucei brucei; Trypanosoma cruzi; Two-Hybrid System Techniques
Año:2009
Volumen:380
Número:4
Página de inicio:850
Página de fin:855
DOI: http://dx.doi.org/10.1016/j.bbrc.2009.01.182
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:amino acid, 65072-01-7; protein, 67254-75-5; Cleavage And Polyadenylation Specificity Factor; mRNA Cleavage and Polyadenylation Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v380_n4_p850_Bercovich

Referencias:

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Citas:

---------- APA ----------
Bercovich, N., Levin, M.J. & Vazquez, M.P. (2009) . The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30. Biochemical and Biophysical Research Communications, 380(4), 850-855.
http://dx.doi.org/10.1016/j.bbrc.2009.01.182
---------- CHICAGO ----------
Bercovich, N., Levin, M.J., Vazquez, M.P. "The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30" . Biochemical and Biophysical Research Communications 380, no. 4 (2009) : 850-855.
http://dx.doi.org/10.1016/j.bbrc.2009.01.182
---------- MLA ----------
Bercovich, N., Levin, M.J., Vazquez, M.P. "The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30" . Biochemical and Biophysical Research Communications, vol. 380, no. 4, 2009, pp. 850-855.
http://dx.doi.org/10.1016/j.bbrc.2009.01.182
---------- VANCOUVER ----------
Bercovich, N., Levin, M.J., Vazquez, M.P. The FIP-1 like polyadenylation factor in trypanosomes and the structural basis for its interaction with CPSF30. Biochem. Biophys. Res. Commun. 2009;380(4):850-855.
http://dx.doi.org/10.1016/j.bbrc.2009.01.182