Artículo
Avila, M.L.; Bercovich, N.; Westergaard, G.; Levin, M.J.; Vázquez, M.P. "Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi" (2007) Biochemical and Biophysical Research Communications. 364(1):26-32
Consulte la política de Acceso Abierto del editor
Abstract:
SF3b155 and p14 are essential components of spliceosome core that recognize the branch point adenosine, a critical step in splicing in eukaryotes. Trypanosomes are unusual since every transcribed gene is processed by trans-splicing instead of cis-splicing. Thus, the trans-spliceosome emerges as an interesting anti-parasitic drug target since this process is not present in mammalian hosts. Here, we present the orthologues of these proteins in Trypanosoma cruzi that interact strongly with each other. To define similarities and differences with the human pair, we performed a detailed alanine scan analysis that allowed us to identify the regions and the critical amino acids of T. cruzi SF3b155 involved in interaction with p14. We demonstrate that the T. cruzi SF3b155 interface is larger and contains more complex elements than its human counterpart. Additionally, our results provide the first insights into the core of the putative mRNA processing complex of trypanosomes. © 2007 Elsevier Inc. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| Autor: | Avila, M.L.; Bercovich, N.; Westergaard, G.; Levin, M.J.; Vázquez, M.P. |
| Filiación: | Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vta. de obligado 2490, Buenos Aires, Argentina Institut Cochin, INSERM U567, Paris, France |
| Palabras clave: | Drug targets; Protein interaction; Trans-splicing; alanine; messenger RNA; protein derivative; protein p14; SF3b155 protein; unclassified drug; article; DNA splicing; gene mapping; nonhuman; priority journal; protein binding; protein processing; spliceosome; Trypanosoma; Trypanosoma cruzi; Amino Acid Sequence; Animals; Humans; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Phosphoproteins; Protein Binding; Protein Structure, Tertiary; Protozoan Proteins; Ribonucleoprotein, U2 Small Nuclear; RNA-Binding Proteins; Spliceosomes; Trypanosoma cruzi; Eukaryota; Mammalia; Trypanosoma cruzi |
| Año: | 2007 |
| Volumen: | 364 |
| Número: | 1 |
| Página de inicio: | 26 |
| Página de fin: | 32 |
| DOI: | http://dx.doi.org/10.1016/j.bbrc.2007.09.090 |
| Título revista: | Biochemical and Biophysical Research Communications |
| Título revista abreviado: | Biochem. Biophys. Res. Commun. |
| ISSN: | 0006291X |
| CODEN: | BBRCA |
| CAS: | alanine, 56-41-7, 6898-94-8; Phosphoproteins; Protozoan Proteins; RNA-Binding Proteins; Ribonucleoprotein, U2 Small Nuclear; SF3B1 protein, human; U2 small nuclear ribonucleoprotein p14 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v364_n1_p26_Avila |
Referencias:
- Clayton, C.E., Life without transcriptional control? From fly to man and back again (2002) EMBO J., 21, pp. 1881-1888
- El-Sayed, N.M., Myler, P.J., Blandin, G., Berriman, M., Crabtree, J., Aggarwal, G., Caler, E., Hall, N., Comparative genomics of trypanosomatid parasitic protozoa (2005) Science, 309, pp. 404-409
- Docampo, R., Sensitivity of parasites to free radical damage by antiparasitic drugs (1990) Chem. Biol. Interact., 73, pp. 1-27
- Liang, X.H., Haritan, A., Uliel, S., Michaeli, S., trans and cis splicing in trypanosomatids: mechanism, factors and regulation (2003) Eukaryot. Cell, 2, pp. 830-840
- Vázquez, M., Atorrasagasti, C., Bercovich, N., Volcovich, R., Levin, M.J., Unique features of the Trypanosoma cruzi U2AF35 splicing factor (2003) Mol. Biochem. Parasitol., 128, pp. 77-81
- Brosi, R., Hauri, H.P., Krämer, A., Separation of splicing factor SF3 into two components and purification of SF3a activity (1993) J. Bio. Chem., 268, pp. 17640-17646
- Cass, D.M., Berglund, J.A., The SF3b155 N-terminal domain is a scaffold important for splicing (2006) Biochemistry, 45, pp. 10092-10101
- Golas, M.M., Sander, B., Will, C.L., Lührmann, R., Stark, H., Molecular architecture of the multiprotein splicing Factor SF3b (2003) Science, 300, pp. 980-984
- Will, C.L., Schneider, C., MacMillan, A.M., Katopodis, N.F., Neubauer, G., Will, M., Lührmann, R., Query, C.C., A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site (2001) EMBO J., 20, pp. 4536-4546
- Spadaccini, R., Reist, U., Dybkov, O., Will, C., Frank, R., Stier, G., Corsini, L., Sattler, M., Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing (2006) RNA, 12, pp. 410-425
- Schellenberg, M.J., Edwards, R.A., Ritchie, D.B., Kent, O.A., Golas, M.M., Stark, H., Lührmann, R., MacMillan, A.M., Crystal structure of core spliceosomal protein interface (2006) Proc. Natl. Acad. Sci. USA, 103, pp. 1266-1271
- Horton, R.M., PCR-mediated recombination and mutagenesis SOEing together tailor-made genes (1995) Mol. Biotechnol., 3, pp. 93-99
- Caro, F., Bercovich, N., Atorrasagasti, C., Levin, M.J., Vázquez, M.P., Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: implications for their functions (2005) Biochem. Biophys. Res. Commun., 333, pp. 1017-1025
- Reed, R., Initial splice site recognition and pairing during pre-mRNA splicing (1996) Curr. Opin. Genet. Dev., 6, pp. 215-220
- Eilbracht, J., Schmidt-Zachmann, M.S., Identification of a sequence element directing a protein to nuclear speckles (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 3849-3854
- Thickman, K.R., Swenson, M.C., Kabogo, J.M., Gryczynski, Z., Kielkopf, C.L., Multiple U2AF65 Binding Sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA Splicing Factors (2006) J. Mol. Biol., 356, pp. 664-683
- Rymond, B., Targeting the spliceosome (2007) Nat. Chem. Biol., 3, pp. 533-535
- Kotake, Y., Sagane, K., Owa, T., Mimori-Kiyosue, Y., Shimizu, H., Uesugi, M., Ishihama, Y., Mizui, Y., Splicing factor SF3b as a target of the antitumor natural product pladienolide (2007) Nat. Chem. Biol., 3, pp. 570-575
- Kaida, D., Motoyoshi, H., Tashiro, E., Nojima, T., Hagiwara, M., Ishigami, K., Watanabe, H., Yoshida, M., Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA (2007) Nat. Chem. Biol., 3, pp. 576-583
- Zeng, J., Mini-review: computational structure-based design of inhibitors that target protein surfaces (2000) Comb. Chem. High Throughput Screen, 3, pp. 355-362
Citas:
---------- APA ----------
Avila, M.L., Bercovich, N., Westergaard, G., Levin, M.J. & Vázquez, M.P. (2007) . Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi. Biochemical and Biophysical Research Communications, 364(1), 26-32.http://dx.doi.org/10.1016/j.bbrc.2007.09.090
---------- CHICAGO ----------
Avila, M.L., Bercovich, N., Westergaard, G., Levin, M.J., Vázquez, M.P. "Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi" . Biochemical and Biophysical Research Communications 364, no. 1 (2007) : 26-32.http://dx.doi.org/10.1016/j.bbrc.2007.09.090
---------- MLA ----------
Avila, M.L., Bercovich, N., Westergaard, G., Levin, M.J., Vázquez, M.P. "Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi" . Biochemical and Biophysical Research Communications, vol. 364, no. 1, 2007, pp. 26-32.http://dx.doi.org/10.1016/j.bbrc.2007.09.090
---------- VANCOUVER ----------
Avila, M.L., Bercovich, N., Westergaard, G., Levin, M.J., Vázquez, M.P. Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 2007;364(1):26-32.http://dx.doi.org/10.1016/j.bbrc.2007.09.090
