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Abstract:

Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2, lacking the acidic cluster, were used to analyze its effect on the interaction with the C subunit, assessed through inhibition of catalytic activity and cAMP activation of reconstituted holoenzyme. A similar decrease in the interaction was obtained when using R1 and R2 with the homologous C subunit; however when using heterologous bovine C, only R1 had a decreased interaction. The results show the general importance of linker I region in the R-C interaction in protein kinases A and point to the importance of the acidic cluster present in the N-terminus of M. circinelloides R subunit in the high affinity interaction between R and C in this holoenzyme. © 2007 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits
Autor:Ocampo, J.; Moreno, S.; Rossi, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y, Naturales, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina
Palabras clave:cAMP; Interaction R-C; Linker I; Mucor circinelloides; PKA; cyclic AMP; cyclic AMP dependent protein kinase; holoenzyme; amino acid sequence; amino terminal sequence; article; catalyst; enzyme activity; enzyme binding; enzyme purification; enzyme subunit; molecular model; Mucor; mucor circinelloides; nonhuman; priority journal; protein expression; protein interaction; Amino Acid Sequence; Animals; Catalytic Domain; Cattle; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Dose-Response Relationship, Drug; Gene Expression Profiling; Kinetics; Molecular Sequence Data; Mucor; Mutagenesis; Mutation; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Bovinae; Fungi; Mucor circinelloides
Año:2007
Volumen:362
Número:3
Página de inicio:721
Página de fin:726
DOI: http://dx.doi.org/10.1016/j.bbrc.2007.08.052
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:cyclic AMP, 60-92-4; Cyclic AMP, 60-92-4; Cyclic AMP-Dependent Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v362_n3_p721_Ocampo

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Citas:

---------- APA ----------
Ocampo, J., Moreno, S. & Rossi, S. (2007) . PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits. Biochemical and Biophysical Research Communications, 362(3), 721-726.
http://dx.doi.org/10.1016/j.bbrc.2007.08.052
---------- CHICAGO ----------
Ocampo, J., Moreno, S., Rossi, S. "PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits" . Biochemical and Biophysical Research Communications 362, no. 3 (2007) : 721-726.
http://dx.doi.org/10.1016/j.bbrc.2007.08.052
---------- MLA ----------
Ocampo, J., Moreno, S., Rossi, S. "PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits" . Biochemical and Biophysical Research Communications, vol. 362, no. 3, 2007, pp. 721-726.
http://dx.doi.org/10.1016/j.bbrc.2007.08.052
---------- VANCOUVER ----------
Ocampo, J., Moreno, S., Rossi, S. PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits. Biochem. Biophys. Res. Commun. 2007;362(3):721-726.
http://dx.doi.org/10.1016/j.bbrc.2007.08.052