Artículo

Rodriguez Granillo, A.; Boffi, J.C.; Barañao, L.; Kordon, E.; Pecci, A.; Guberman, A. "STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line" (2007) Biochemical and Biophysical Research Communications. 356(3):727-732
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Abstract:

Gene regulation mediated by STAT factors has been implicated in cellular functions with relevance to a variety of processes. Particularly, STAT5 and STAT3 play a crucial role in mammary epithelium displaying reciprocal activation kinetics during pregnancy, lactation and involution. Here, we show that LIF treatment of mammary epithelial HC11 cells reduces the phosphorylation levels and transcriptional activity of p-STAT5 in correlation with STAT3 phosphorylation. We have also found that STAT5 activity is negatively modulated by this cytokine, both on a gene whose expression is induced, as well as on a promoter repressed by STAT5. Besides, our results show that lactogenic hormones increase LIF effect on gene induction without modifying STAT3 phosphorylation state. Our findings strongly suggest that there is crosstalk between STAT5 and STAT3 pathways that could modulate their ability to regulate gene expression. © 2007 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line
Autor:Rodriguez Granillo, A.; Boffi, J.C.; Barañao, L.; Kordon, E.; Pecci, A.; Guberman, A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Piso 2, C1428EGA Buenos Aires, Argentina
IBYME, CONICET, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
IFIBYNE, CONICET, Pab. II, C1428EGA Buenos Aires, Argentina
Palabras clave:Gene expression; HC11; Involution; LIF; Mammary epithelium; Prolactin; Signal transduction; STAT3; STAT5; leukemia inhibitory factor; prolactin; STAT3 protein; STAT5 protein; article; breast epithelium; controlled study; gene control; gene expression regulation; gene repression; human; human cell; involution; priority journal; promoter region; protein phosphorylation; signal transduction; transcription regulation; Animals; bcl-X Protein; CCAAT-Enhancer-Binding Protein-delta; Dexamethasone; Epithelium; Insulin; Leukemia Inhibitory Factor; Mammary Glands, Animal; Mice; Phosphorylation; Prolactin; Promoter Regions (Genetics); Proto-Oncogene Proteins c-fos; STAT3 Transcription Factor; STAT5 Transcription Factor
Año:2007
Volumen:356
Número:3
Página de inicio:727
Página de fin:732
DOI: http://dx.doi.org/10.1016/j.bbrc.2007.03.040
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:prolactin, 12585-34-1, 50647-00-2, 9002-62-4; bcl-X Protein; Bcl2l1 protein, mouse; CCAAT-Enhancer-Binding Protein-delta, 142662-43-9; Dexamethasone, 50-02-2; Insulin, 11061-68-0; Leukemia Inhibitory Factor; Lif protein, mouse; Prolactin, 9002-62-4; Proto-Oncogene Proteins c-fos; Stat3 protein, mouse; STAT3 Transcription Factor; STAT5 Transcription Factor; Stat5a protein, mouse; Stat5b protein, mouse
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v356_n3_p727_RodriguezGranillo

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Citas:

---------- APA ----------
Rodriguez Granillo, A., Boffi, J.C., Barañao, L., Kordon, E., Pecci, A. & Guberman, A. (2007) . STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line. Biochemical and Biophysical Research Communications, 356(3), 727-732.
http://dx.doi.org/10.1016/j.bbrc.2007.03.040
---------- CHICAGO ----------
Rodriguez Granillo, A., Boffi, J.C., Barañao, L., Kordon, E., Pecci, A., Guberman, A. "STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line" . Biochemical and Biophysical Research Communications 356, no. 3 (2007) : 727-732.
http://dx.doi.org/10.1016/j.bbrc.2007.03.040
---------- MLA ----------
Rodriguez Granillo, A., Boffi, J.C., Barañao, L., Kordon, E., Pecci, A., Guberman, A. "STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line" . Biochemical and Biophysical Research Communications, vol. 356, no. 3, 2007, pp. 727-732.
http://dx.doi.org/10.1016/j.bbrc.2007.03.040
---------- VANCOUVER ----------
Rodriguez Granillo, A., Boffi, J.C., Barañao, L., Kordon, E., Pecci, A., Guberman, A. STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line. Biochem. Biophys. Res. Commun. 2007;356(3):727-732.
http://dx.doi.org/10.1016/j.bbrc.2007.03.040