Artículo

Maskin, L.; Frankel, N.; Gudesblat, G.; Demergasso, M.J.; Pietrasanta, L.I.; Iusem, N.D. "Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss" (2007) Biochemical and Biophysical Research Communications. 352(4):831-835
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Abstract:

The Asr gene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss. In vitro, electrophoretic assays show a homo-dimeric structure for ASR1 and highlight strong non-covalent interactions between monomers prone to self-assemble. Direct visualization of single molecules by atomic force microscopy (AFM) confirms that ASR1 forms homodimers and that uncovers both monomers and dimers bind double stranded DNA. © 2006 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss
Autor:Maskin, L.; Frankel, N.; Gudesblat, G.; Demergasso, M.J.; Pietrasanta, L.I.; Iusem, N.D.
Filiación:Laboratorio de Fisiología y Biología Molecular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina
Centro de Microscopías Avanzadas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Asr genes; Atomic force microscopy; Dimerization; Lycopersicon esculentum; Seed; dimer; double stranded DNA; homodimer; monomer; protein asr1; vegetable protein; article; atomic force microscopy; chemical bond; dimerization; DNA binding; electrophoresis; embryo development; gene activation; hydrophilicity; in vitro study; multigene family; nonhuman; plant seed; plant tissue; priority journal; protein assembly; protein DNA interaction; protein structure; water loss; Dimerization; DNA-Binding Proteins; Lycopersicon esculentum; Microscopy, Atomic Force; Plant Proteins; Plasmids; Protein Binding; Seeds; Water; Lycopersicon esculentum; Spermatophyta
Año:2007
Volumen:352
Número:4
Página de inicio:831
Página de fin:835
DOI: http://dx.doi.org/10.1016/j.bbrc.2006.11.115
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:DNA-Binding Proteins; Plant Proteins; Water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v352_n4_p831_Maskin

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Citas:

---------- APA ----------
Maskin, L., Frankel, N., Gudesblat, G., Demergasso, M.J., Pietrasanta, L.I. & Iusem, N.D. (2007) . Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss. Biochemical and Biophysical Research Communications, 352(4), 831-835.
http://dx.doi.org/10.1016/j.bbrc.2006.11.115
---------- CHICAGO ----------
Maskin, L., Frankel, N., Gudesblat, G., Demergasso, M.J., Pietrasanta, L.I., Iusem, N.D. "Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss" . Biochemical and Biophysical Research Communications 352, no. 4 (2007) : 831-835.
http://dx.doi.org/10.1016/j.bbrc.2006.11.115
---------- MLA ----------
Maskin, L., Frankel, N., Gudesblat, G., Demergasso, M.J., Pietrasanta, L.I., Iusem, N.D. "Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss" . Biochemical and Biophysical Research Communications, vol. 352, no. 4, 2007, pp. 831-835.
http://dx.doi.org/10.1016/j.bbrc.2006.11.115
---------- VANCOUVER ----------
Maskin, L., Frankel, N., Gudesblat, G., Demergasso, M.J., Pietrasanta, L.I., Iusem, N.D. Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss. Biochem. Biophys. Res. Commun. 2007;352(4):831-835.
http://dx.doi.org/10.1016/j.bbrc.2006.11.115