Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Trypanosoma cruzi, the etiological agent of Chagas' disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The transporter gene TcPAT12 was functionally expressed in Xenopus laevis oocytes, showing high levels of spermidine uptake. Similar apparent affinity constants for spermidine uptake were obtained when comparing T. cruzi epimastigotes and heterologous expressed TcPAT12 in X. laevis. In addition, TcPAT12 also transports putrescine and the amino acid l-arginine at lower rates than spermidine. © 2006 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi
Autor:Carrillo, C.; Canepa, G.E.; Algranati, I.D.; Pereira, C.A.
Filiación:Fundación Instituto Leloir, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Departamento de Sustancias Vasoactivas, Instituto de Investigaciones Médicas Alfredo Lanari, Buenos Aires, Argentina
Palabras clave:Arginine; Chagas' disease; Permease; Polyamines; Putrescine; Spermidine; Trypanosoma cruzi; amino acid; arginine; polyamine; putrescine; spermidine; spermidine transporter; unclassified drug; article; epimastigote; gene expression; nucleotide sequence; polyamine synthesis; priority journal; protein determination; protein expression; protein transport; Trypanosoma cruzi; Xenopus laevis; Amino Acid Sequence; Animals; Molecular Sequence Data; Protein Binding; Protozoan Proteins; Spermidine; Trypanosoma cruzi; Eukaryota; Trypanosoma cruzi; Xenopus laevis
Año:2006
Volumen:344
Número:3
Página de inicio:936
Página de fin:940
DOI: http://dx.doi.org/10.1016/j.bbrc.2006.03.215
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:amino acid, 65072-01-7; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; Protozoan Proteins; Spermidine, 124-20-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v344_n3_p936_Carrillo

Referencias:

  • Sanchez, M.A., Ullman, B., Landfear, S.M., Carter, N.S., Cloning and functional expression of a gene encoding a P1 type nucleoside transporter from Trypanosoma brucei (1999) J. Biol. Chem., 274, pp. 30244-30249
  • Aronow, B., Kaur, K., Mc Cartan, K., Ullman, B., Two high affinity nucleoside transporters in Leishmania donovani (1987) Mol. Biochem. Parasitol., 22, pp. 29-37
  • Vasudevan, G., Ullman, B., Landfear, S., Point mutations in a nucleoside transporter gene from Leishmania donovani confer drug resistance and alter substrate selectivity (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 6092-6097
  • Maser, P., Sutterlin, C., Kralli, A., Kaminsky, R., A nucleoside transporter from Trypanosoma brucei involved in drug resistance (1999) Science, 285, pp. 242-244
  • Matovu, E., Stewart, M.L., Geiser, F., Brun, R., Maser, P., Wallace, L.J., Burchmore, R.J., de Koning, H.P., Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei (2003) Eukaryot. Cell, 2, pp. 1003-1008
  • Wallace, H.M., Fraser, A.V., Hughes, A., A perspective of polyamine metabolism (2003) Biochem. J., 376, pp. 1-14
  • Oza, S.L., Tetaud, E., Ariyanayagam, M.R., Warnon, S.S., Fairlamb, A.H., A single enzyme catalyses formation of Trypanothione from glutathione and spermidine in Trypanosoma cruzi (2002) J. Biol. Chem., 277, pp. 35853-35861
  • Carrillo, C., Cejas, S., Huber, A., Gonzalez, N.S., Algranati, I.D., Lack of arginine decarboxylase in Trypanosoma cruzi epimastigotes (2003) J. Eukaryot. Microbiol., 50, pp. 12-16
  • Carrillo, C., Cejas, S., Gonzalez, N.S., Algranati, I.D., Trypanosoma cruzi epimastigotes lack ornithine decarboxylase but can express a foreign gene encoding this enzyme (1999) FEBS Lett., 454, pp. 192-196
  • Bacchi, C.J., Nathan, H.C., Hutner, S.H., Mc Cann, P.P., Sjoerdsma, A., Polyamine metabolism: a potential therapeutic target in trypanosomes (1980) Science, 210, pp. 332-334
  • Bacchi, C.J., Mc Cann, P.P., Parasitic protozoa and polyamines (1987) Inhibition of Polyamine Metabolism. Biological Significance and Bases for New Therapies, pp. 317-344. , Mc Cann P.P., Pegg A.E., and Sjoerdsma A. (Eds), Academic Press, Orlando, FL
  • Van Nieuwenhove, S., Schechter, P.J., Declercq, J., Boné, G., Burke, J., Sjoerdsma, A., Treatment of gambiense sleeping sickness in the Sudan with oral DFMO, an inhibitor of ornithine decarboxylase: first field trial (1985) Trans. R. Soc. Trop. Med. Hyg., 79, pp. 692-698
  • Le Quesne, S.A., Fairlamb, A.H., Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes (1996) Biochem. J., 316, pp. 481-486
  • Gonzalez, N.S., Ceriani, C., Algranati, I.D., Differential regulation of putrescine uptake in Trypanosoma cruzi and other trypanosomatids (1992) Biochem. Biophys. Res. Commun., 188, pp. 120-128
  • Hasne, M.P., Ullman, B., Identification and characterization of a polyamine permease from the protozoan parasite Leishmania major (2005) J. Biol. Chem., 280, pp. 15188-15194
  • Bouvier, L.A., Silber, A.M., Galvao Lopes, C., Canepa, G.E., Miranda, M.R., Tonelli, R.R., Colli, W., Pereira, C.A., Post genomic analysis of permeases from the amino acid/auxin family in protozoan parasites (2004) Biochem. Biophys. Res. Commun., 321, pp. 547-556
  • Liman, E.R., Tytgat, J., Hess, P., Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs (1992) Neuron, 9, pp. 861-871
  • Czibener, C., Alvarez, D., Scodeller, E., Gamarnik, A.V., Characterization of internal ribosomal entry sites of Triatoma virus (2005) J. Gen. Virol., 86, pp. 2275-2280
  • Bertran, J., Werner, A., Stange, G., Markovich, D., Biber, J., Testar, X., Zorzano, A., Murer, H., Expression of Na(+)-independent amino acid transport in Xenopus laevis oocytes by injection of rabbit kidney cortex mRNA (1992) Biochem. J., 281, pp. 717-723
  • Dixon, M., Webb, E.C., (1964) Enzymes. 2nd ed., , Longmans, Green and Co., London pp. 67-70
  • Contreras, V.T., Salles, J.M., Thomas, N., Morel, C.M., Goldenberg, S., In vitro differentiation of Trypanosoma cruzi under chemically defined conditions (1985) Mol. Biochem. Parasitol., 16, pp. 315-327
  • Machado, C.A., Ayala, F.J., Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 7396-7401
  • Aouida, M., Leduc, A., Poulin, R., Ramotar, D., AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae (2005) J. Biol. Chem., 280, pp. 24267-24276

Citas:

---------- APA ----------
Carrillo, C., Canepa, G.E., Algranati, I.D. & Pereira, C.A. (2006) . Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi. Biochemical and Biophysical Research Communications, 344(3), 936-940.
http://dx.doi.org/10.1016/j.bbrc.2006.03.215
---------- CHICAGO ----------
Carrillo, C., Canepa, G.E., Algranati, I.D., Pereira, C.A. "Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi" . Biochemical and Biophysical Research Communications 344, no. 3 (2006) : 936-940.
http://dx.doi.org/10.1016/j.bbrc.2006.03.215
---------- MLA ----------
Carrillo, C., Canepa, G.E., Algranati, I.D., Pereira, C.A. "Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi" . Biochemical and Biophysical Research Communications, vol. 344, no. 3, 2006, pp. 936-940.
http://dx.doi.org/10.1016/j.bbrc.2006.03.215
---------- VANCOUVER ----------
Carrillo, C., Canepa, G.E., Algranati, I.D., Pereira, C.A. Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 2006;344(3):936-940.
http://dx.doi.org/10.1016/j.bbrc.2006.03.215