Artículo

Malicet, C.; Hoffmeister, A.; Moreno, S.; Closa, D.; Dagorn, J.-C.; Vasseur, S.; Iovanna, J.L. "Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation" (2006) Biochemical and Biophysical Research Communications. 339(1):284-289
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Abstract:

p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activity. We used two-hybrid screening of a pre-transformed human testes cDNA library to identify some of these partners. One of them was the multifunctional protein Jab1, its interaction with p8 being confirmed by His6-pull down and co-immunoprecipitation assays. In addition, we could show that the two proteins co-localized in the cell. Our functional data demonstrate that Jab1 requires direct interaction with p8 to induce the translocation of p27 from nucleus to cytoplasm and its subsequent degradation. Experiments showing that the knock-down of p8 expression results in a strong inhibition of Jab1 activity confirmed that the mechanism by which Jab1 promotes cell growth by decreasing p27 level is p8-dependent. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation
Autor:Malicet, C.; Hoffmeister, A.; Moreno, S.; Closa, D.; Dagorn, J.-C.; Vasseur, S.; Iovanna, J.L.
Filiación:INSERM U.624, Stress Cellulaire, Parc Scientifique et Technologique de Luminy, 163 Avenue de Luminy, Case 915, 13288 Marseille Cedex 9, France
Departamento de Química Biológica, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Experimental Pathology Department, IIBB-SCIC, IDIBAPS, Barcelona, Spain
Palabras clave:Cell cycle; Jab1; p27; p8; Two hybrid; binding protein; complementary DNA; heat shock protein; heat shock protein p 8; histidine; jun activating binding 1 protein; protein p27; unclassified drug; article; cell growth; cellular distribution; controlled study; DNA library; enzyme activity; enzyme inhibition; HeLa cell; human; human cell; hybrid cell; immunoprecipitation; polyacrylamide gel electrophoresis; priority journal; protein degradation; protein expression; protein function; protein induction; protein localization; protein protein interaction; protein synthesis inhibition; protein synthesis regulation
Año:2006
Volumen:339
Número:1
Página de inicio:284
Página de fin:289
DOI: http://dx.doi.org/10.1016/j.bbrc.2005.11.018
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:histidine, 645-35-2, 7006-35-1, 71-00-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v339_n1_p284_Malicet

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Citas:

---------- APA ----------
Malicet, C., Hoffmeister, A., Moreno, S., Closa, D., Dagorn, J.-C., Vasseur, S. & Iovanna, J.L. (2006) . Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation. Biochemical and Biophysical Research Communications, 339(1), 284-289.
http://dx.doi.org/10.1016/j.bbrc.2005.11.018
---------- CHICAGO ----------
Malicet, C., Hoffmeister, A., Moreno, S., Closa, D., Dagorn, J.-C., Vasseur, S., et al. "Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation" . Biochemical and Biophysical Research Communications 339, no. 1 (2006) : 284-289.
http://dx.doi.org/10.1016/j.bbrc.2005.11.018
---------- MLA ----------
Malicet, C., Hoffmeister, A., Moreno, S., Closa, D., Dagorn, J.-C., Vasseur, S., et al. "Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation" . Biochemical and Biophysical Research Communications, vol. 339, no. 1, 2006, pp. 284-289.
http://dx.doi.org/10.1016/j.bbrc.2005.11.018
---------- VANCOUVER ----------
Malicet, C., Hoffmeister, A., Moreno, S., Closa, D., Dagorn, J.-C., Vasseur, S., et al. Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation. Biochem. Biophys. Res. Commun. 2006;339(1):284-289.
http://dx.doi.org/10.1016/j.bbrc.2005.11.018