Artículo

Shayo, C.; Legnazzi, B.L.; Monczor, F.; Fernández, N.; Riveiro, M.E.; Baldi, A.; Davio, C. "The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation" (2004) Biochemical and Biophysical Research Communications. 314(3):798-804
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Abstract:

The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to analyze the correlation between cAMP time-course and cell differentiation. All three agents denoted similar cAMP maximal responses in dose-response experiments. The kinetic of desensitization showed differential characteristics, while H2 receptor desensitized homologously without affecting PGE2 or forskolin effect, PGE2 response showed mixed desensitization characterized by a homologous initial phase followed by a heterologous phase. Regarding forskolin, long-term stimuli attenuated PGE2 and H2 agonist response without affecting adenylyl cyclase activity. In the absence of phosphodiesterase inhibitors, the three agents induced similar maximal cAMP levels after 5min, but only that induced by the H2 agonist returned to basal levels. Consistent with this observation, H2 agonist was not able to induce U-937 cell maturation in contrast to PGE2 and forskolin, supporting the importance of time-course signaling in the determination of cell behavior. © 2003 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
Autor:Shayo, C.; Legnazzi, B.L.; Monczor, F.; Fernández, N.; Riveiro, M.E.; Baldi, A.; Davio, C.
Filiación:Inst. de Biol. y Med. Experimental, Buenos Aires, Argentina
Laboratorio de Radioisótopos, Fac. de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
Depto. Fisiol., Biol. Molec. y Cel., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Consejo Nac. de Invest. Cie. y Tec., Buenos Aires, Argentina
Palabras clave:cAMP signaling; Cell differentiation; Leukemia; amthamine; calcium; complement component C5a; cyclic AMP; forskolin; histamine agonist; histamine H2 receptor; Myc protein; prostaglandin E receptor; prostaglandin E2; prostaglandin receptor stimulating agent; protein c fos; recombinant protein; thiazole derivative; article; biosynthesis; cell differentiation; cell division; cell maturation; cell strain U937; controlled study; correlation analysis; desensitization; dose response; drug effect; enzyme activity; genetics; human; human cell; kinetics; leukemia cell; metabolism; physiology; priority journal; sequence homology; signal transduction; time
Año:2004
Volumen:314
Número:3
Página de inicio:798
Página de fin:804
DOI: http://dx.doi.org/10.1016/j.bbrc.2003.12.166
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:amthamine, 142437-67-0; calcium, 7440-70-2, 14092-94-5; cyclic AMP, 60-92-4; forskolin, 66575-29-9; prostaglandin E2, 363-24-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v314_n3_p798_Shayo

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Citas:

---------- APA ----------
Shayo, C., Legnazzi, B.L., Monczor, F., Fernández, N., Riveiro, M.E., Baldi, A. & Davio, C. (2004) . The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation. Biochemical and Biophysical Research Communications, 314(3), 798-804.
http://dx.doi.org/10.1016/j.bbrc.2003.12.166
---------- CHICAGO ----------
Shayo, C., Legnazzi, B.L., Monczor, F., Fernández, N., Riveiro, M.E., Baldi, A., et al. "The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation" . Biochemical and Biophysical Research Communications 314, no. 3 (2004) : 798-804.
http://dx.doi.org/10.1016/j.bbrc.2003.12.166
---------- MLA ----------
Shayo, C., Legnazzi, B.L., Monczor, F., Fernández, N., Riveiro, M.E., Baldi, A., et al. "The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation" . Biochemical and Biophysical Research Communications, vol. 314, no. 3, 2004, pp. 798-804.
http://dx.doi.org/10.1016/j.bbrc.2003.12.166
---------- VANCOUVER ----------
Shayo, C., Legnazzi, B.L., Monczor, F., Fernández, N., Riveiro, M.E., Baldi, A., et al. The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation. Biochem. Biophys. Res. Commun. 2004;314(3):798-804.
http://dx.doi.org/10.1016/j.bbrc.2003.12.166