Artículo

Shizuka-Ikeda, M.; Matsubara, E.; Ikeda, M.; Kanai, M.; Tomidokoro, Y.; Ikeda, Y.; Watanabe, M.; Kawarabayashi, T.; Harigaya, Y.; Okamoto, K.; Shoji, M.; Maruyama, K.; Castao, E.M.; St. George-Hyslop, P. "Generation of amyloid β protein from a presenilin-1 and βAPP complex" (2002) Biochemical and Biophysical Research Communications. 292(2):571-578
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Abstract:

Presenilin-1 (PS1) is a causative gene in early onset familial Alzheimer’s disease (FAD). FAD-linked mutant PS1s significantly increased Aβ40 and Aβ42(43) levels (P < 0.001) and decreased the production of an 11.4 kD (β-stub) and an 8.7 kD (α-stub) carboxyl-terminal fragment of amyloid β precursor protein (βAPP-CTFs) (P < 0.01). In the 2% CHAPS extracted lysates, the complex containing the amino-terminal fragment of PS1 (PS1-NTF), the carboxyl-terminal fragments of PS1 (PS1-CTF), and βAPP-CTFs was identified. Incubation of this isolated complex at pH 6.4 showed the direct generation of Aβ40 and γ-stub from this complex. This reaction was inhibited by a β-secretase inhibitor. The degrading rate of a co-precipitated β-stub was facilitated under the presence of FAD-linked mutant PS1s. This findings suggest that the direct generation of Aβ from the complex may play an important role in the pathogenesis of Alzheimer’s disease. © 2002 Elsevier Science (USA).

Registro:

Documento: Artículo
Título:Generation of amyloid β protein from a presenilin-1 and βAPP complex
Autor:Shizuka-Ikeda, M.; Matsubara, E.; Ikeda, M.; Kanai, M.; Tomidokoro, Y.; Ikeda, Y.; Watanabe, M.; Kawarabayashi, T.; Harigaya, Y.; Okamoto, K.; Shoji, M.; Maruyama, K.; Castao, E.M.; St. George-Hyslop, P.
Filiación:Department of Neurology, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan
Department of Pharmacology, Saitama Medical School, Moroyama, Saitama, 350-0495, Japan
Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), Departamento de Química Biológica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina
Centre for Research in Neurodegenerative Diseases, Department of Medicine, University of Toronto, Toronto, Ont., Canada
Palabras clave:Aβ40; Aβ42(43); Presenilin-1; PS1/βAPP complex; βAPP; γ-secretase; 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid; amyloid beta protein; enzyme inhibitor; gamma secretase; mutant protein; presenilin 1; protein precursor; Alzheimer disease; amino terminal sequence; article; carboxy terminal sequence; cell line; cell lysate; pathogenesis; pH; precipitation; priority journal; protein analysis; protein degradation; protein isolation; reaction analysis
Año:2002
Volumen:292
Número:2
Página de inicio:571
Página de fin:578
DOI: http://dx.doi.org/10.1006/bbrc.2002.6681
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CAS:3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid, 75621-03-3; amyloid beta protein, 109770-29-8; presenilin 1, 186986-30-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v292_n2_p571_ShizukaIkeda

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Citas:

---------- APA ----------
Shizuka-Ikeda, M., Matsubara, E., Ikeda, M., Kanai, M., Tomidokoro, Y., Ikeda, Y., Watanabe, M.,..., St. George-Hyslop, P. (2002) . Generation of amyloid β protein from a presenilin-1 and βAPP complex. Biochemical and Biophysical Research Communications, 292(2), 571-578.
http://dx.doi.org/10.1006/bbrc.2002.6681
---------- CHICAGO ----------
Shizuka-Ikeda, M., Matsubara, E., Ikeda, M., Kanai, M., Tomidokoro, Y., Ikeda, Y., et al. "Generation of amyloid β protein from a presenilin-1 and βAPP complex" . Biochemical and Biophysical Research Communications 292, no. 2 (2002) : 571-578.
http://dx.doi.org/10.1006/bbrc.2002.6681
---------- MLA ----------
Shizuka-Ikeda, M., Matsubara, E., Ikeda, M., Kanai, M., Tomidokoro, Y., Ikeda, Y., et al. "Generation of amyloid β protein from a presenilin-1 and βAPP complex" . Biochemical and Biophysical Research Communications, vol. 292, no. 2, 2002, pp. 571-578.
http://dx.doi.org/10.1006/bbrc.2002.6681
---------- VANCOUVER ----------
Shizuka-Ikeda, M., Matsubara, E., Ikeda, M., Kanai, M., Tomidokoro, Y., Ikeda, Y., et al. Generation of amyloid β protein from a presenilin-1 and βAPP complex. Biochem. Biophys. Res. Commun. 2002;292(2):571-578.
http://dx.doi.org/10.1006/bbrc.2002.6681