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Bocca, S.N.; Muzzopappa, M.; Silberstein, S.; Wappner, P. "Occurrence of a putative SCF ubiquitin ligase complex in Drosophila" (2001) Biochemical and Biophysical Research Communications. 286(2):357-364
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Abstract:

Many proteins are targeted to proteasome degradation by a family of E3 ubiquitin ligases, termed SCF complexes, that link substrate proteins to an E2 ubiquitin-conjugating enzyme. SCFs are composed of three core proteins-Skp1, Cdc53/Cull, Rbx1/Hrt1-and a substrate specific F-box protein. We have identified in Drosophila melanogaster the closest homologues to the human components of the SCFβTrCP complex and the E2 ubiquitin-conjugating enzyme UbcH5. We show that putative Drosophila SCF core subunits dSkpA and dRbx1 both interact directly with dCu11 and the F-box protein Slmb. We also describe the direct interaction of the UbcH5 related protein UbcD1 with dCul1 and Slmb. In addition, a functional complementation test performed on a Saccharomyces cerevisiae Hrt1p-deficient mutant showed that Drosophila Rbx1 is able to restore the yeast cells viability. Our results suggest that dRbx1, dSkpA, dCullin1, and Slimb proteins are components of a Drosophila SCF complex that functions in combination with the ubiquitin conjugating enzyme UbcD1. © 2001 Academic Press.

Registro:

Documento: Artículo
Título:Occurrence of a putative SCF ubiquitin ligase complex in Drosophila
Autor:Bocca, S.N.; Muzzopappa, M.; Silberstein, S.; Wappner, P.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, 1405, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad de San Martin, San Martin, Pcia. de Buenos Aires, Argentina
Palabras clave:Drosophila; E3 ligase; Proteolysis; SCF; Ubiquitin; ligase; proteasome; ubiquitin; article; Drosophila; Drosophila melanogaster; enzyme activity; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; protein analysis; protein degradation; protein interaction; protein purification; Saccharomyces cerevisiae
Año:2001
Volumen:286
Número:2
Página de inicio:357
Página de fin:364
DOI: http://dx.doi.org/10.1006/bbrc.2001.5394
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:ligase, 9080-13-1; proteasome, 140879-24-9; ubiquitin, 60267-61-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v286_n2_p357_Bocca

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Citas:

---------- APA ----------
Bocca, S.N., Muzzopappa, M., Silberstein, S. & Wappner, P. (2001) . Occurrence of a putative SCF ubiquitin ligase complex in Drosophila. Biochemical and Biophysical Research Communications, 286(2), 357-364.
http://dx.doi.org/10.1006/bbrc.2001.5394
---------- CHICAGO ----------
Bocca, S.N., Muzzopappa, M., Silberstein, S., Wappner, P. "Occurrence of a putative SCF ubiquitin ligase complex in Drosophila" . Biochemical and Biophysical Research Communications 286, no. 2 (2001) : 357-364.
http://dx.doi.org/10.1006/bbrc.2001.5394
---------- MLA ----------
Bocca, S.N., Muzzopappa, M., Silberstein, S., Wappner, P. "Occurrence of a putative SCF ubiquitin ligase complex in Drosophila" . Biochemical and Biophysical Research Communications, vol. 286, no. 2, 2001, pp. 357-364.
http://dx.doi.org/10.1006/bbrc.2001.5394
---------- VANCOUVER ----------
Bocca, S.N., Muzzopappa, M., Silberstein, S., Wappner, P. Occurrence of a putative SCF ubiquitin ligase complex in Drosophila. Biochem. Biophys. Res. Commun. 2001;286(2):357-364.
http://dx.doi.org/10.1006/bbrc.2001.5394