Artículo

Bollo, M.; Venera, G.; de Jiménez Bonino, M.B.; Machado-Domenech, E. "Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi" (2001) Biochemical and Biophysical Research Communications. 281(2):300-304
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Abstract:

This work provides evidence that nicotine (1×10-5M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6×10-7M) and nicotine (IC50: 1×10-7M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. © 2001 Academic Press.

Registro:

Documento: Artículo
Título:Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi
Autor:Bollo, M.; Venera, G.; de Jiménez Bonino, M.B.; Machado-Domenech, E.
Filiación:Química Biológica, Facultad de Ciencias Exactas Fisico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas UBA-CONICET, Junín 956, 1113 Buenos Aires, Argentina
Palabras clave:Calcium; Carbamylcholine; Nicotine; Nicotinic ligands; Trypanosoma cruzi; α-bungarotoxin; alpha bungarotoxin; carbachol; cholinergic receptor; nicotine; article; binding affinity; calcium cell level; calcium signaling; epimastigote; IC 50; ligand binding; membrane binding; nonhuman; priority journal; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:2001
Volumen:281
Número:2
Página de inicio:300
Página de fin:304
DOI: http://dx.doi.org/10.1006/bbrc.2001.4344
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CAS:alpha bungarotoxin, 11032-79-4; carbachol, 462-58-8, 51-83-2; nicotine, 54-11-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v281_n2_p300_Bollo

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Citas:

---------- APA ----------
Bollo, M., Venera, G., de Jiménez Bonino, M.B. & Machado-Domenech, E. (2001) . Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi. Biochemical and Biophysical Research Communications, 281(2), 300-304.
http://dx.doi.org/10.1006/bbrc.2001.4344
---------- CHICAGO ----------
Bollo, M., Venera, G., de Jiménez Bonino, M.B., Machado-Domenech, E. "Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi" . Biochemical and Biophysical Research Communications 281, no. 2 (2001) : 300-304.
http://dx.doi.org/10.1006/bbrc.2001.4344
---------- MLA ----------
Bollo, M., Venera, G., de Jiménez Bonino, M.B., Machado-Domenech, E. "Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi" . Biochemical and Biophysical Research Communications, vol. 281, no. 2, 2001, pp. 300-304.
http://dx.doi.org/10.1006/bbrc.2001.4344
---------- VANCOUVER ----------
Bollo, M., Venera, G., de Jiménez Bonino, M.B., Machado-Domenech, E. Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 2001;281(2):300-304.
http://dx.doi.org/10.1006/bbrc.2001.4344