Artículo

Carrillo, C.; Cejas, S.; Cortés, M.; Ceriani, C.; Huber, A.; González, N.S.; Algranati, I.D. "Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase" (2000) Biochemical and Biophysical Research Communications. 279(2):663-668
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Abstract:

α-Difluoromethylornithine (DFMO), the specific and irreversible inhibitor of ornithine decarboxylase (ODC), was able to induce the arrest of proliferation in Leishmania mexicana and ODC-transformed Trypanosoma cruzi cultures grown in a semi-defined medium essentially free of polyamines. Conversely, Crithidia fasciculata and Phytomonas 274 were not affected by the inhibitor. The drug-resistance of Crithidia and Phytomonas was neither caused by an impairment of DFMO uptake nor by a decrease of the enzyme affinity for the inhibitor. We were also able to rule out the possibility of ODC overexpression in the drug-tolerant parasites. The measurements of ODC metabolic turnover indicated that the enzymes from Crithidia and Phytomonas have a short half-life of 20-40 min, while ODC from Leishmania and transgenic Trypanosoma cruzi are rather stable with a half-life longer than 6 hours. Analyses of polyamine internal pools under different growth conditions have shown that DFMO was able to markedly decrease the levels of putrescine and spermidine in all parasites, but the depletion of spermidine was higher in trypanosomatids containing an ODC with slow turnover. Our results suggest that in these parasites cultivated in the presence of the drug, spermidine might decrease below critical levels needed to maintain trypanothione concentrations or other conditions essential for normal proliferation. © 2000 Academic Press.

Registro:

Documento: Artículo
Título:Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase
Autor:Carrillo, C.; Cejas, S.; Cortés, M.; Ceriani, C.; Huber, A.; González, N.S.; Algranati, I.D.
Filiación:Instituto de Invest. Bioquím., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires/CONICET, A. Machado 151, 1405 Buenos Aires, Argentina
Palabras clave:Crithidia fasciculata; DFMO-resistance; Leishmania mexicana; Ornithine decarboxylase; Phytomonas; Polyamines; Trypanosoma cruzi; Trypanosomatids proliferation; eflornithine; ornithine decarboxylase; spermidine; trypanothione; antiprotozoal activity; article; Crithidia fasciculata; drug effect; drug screening; enzyme inhibition; enzyme metabolism; Leishmania mexicana; nonhuman; Phytomonas; priority journal; Trypanosoma cruzi
Año:2000
Volumen:279
Número:2
Página de inicio:663
Página de fin:668
DOI: http://dx.doi.org/10.1006/bbrc.2000.3996
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:eflornithine, 67037-37-0, 70052-12-9; ornithine decarboxylase, 9024-60-6; spermidine, 124-20-9, 334-50-9; trypanothione, 96304-42-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v279_n2_p663_Carrillo

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Citas:

---------- APA ----------
Carrillo, C., Cejas, S., Cortés, M., Ceriani, C., Huber, A., González, N.S. & Algranati, I.D. (2000) . Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase. Biochemical and Biophysical Research Communications, 279(2), 663-668.
http://dx.doi.org/10.1006/bbrc.2000.3996
---------- CHICAGO ----------
Carrillo, C., Cejas, S., Cortés, M., Ceriani, C., Huber, A., González, N.S., et al. "Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase" . Biochemical and Biophysical Research Communications 279, no. 2 (2000) : 663-668.
http://dx.doi.org/10.1006/bbrc.2000.3996
---------- MLA ----------
Carrillo, C., Cejas, S., Cortés, M., Ceriani, C., Huber, A., González, N.S., et al. "Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase" . Biochemical and Biophysical Research Communications, vol. 279, no. 2, 2000, pp. 663-668.
http://dx.doi.org/10.1006/bbrc.2000.3996
---------- VANCOUVER ----------
Carrillo, C., Cejas, S., Cortés, M., Ceriani, C., Huber, A., González, N.S., et al. Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase. Biochem. Biophys. Res. Commun. 2000;279(2):663-668.
http://dx.doi.org/10.1006/bbrc.2000.3996