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Cardalda, C.A.; Batlle, A.; Juknat, A.A. "Sequence and structure of the rat housekeeping PBG-D isoform" (1998) Biochemical and Biophysical Research Communications. 249(2):438-443
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Abstract:

Porphobilinogen deaminase (PBG-D), a key enzyme in the tetrapyrrole biosynthetic pathway, is encoded by a single gene containing two different promoters. The upstream promoter, found in all cell types, initiates the transcription of the housekeeping PBG-D isoform, whereas the downstream one is erythroid-specific. In this study, we provide the first full sequence of a 1086 bp cDNA covering the coding region for the rat ubiquitous PBG-D and its primary amino acid sequence. The cDNA encodes a 39,361 Da protein composed of 361 amino acids. Nucleotide sequence comparison between both isoforms from rat shows similarities of 99.5%, with four changes (C/G) in exon 8 and only one (C/A) in exon 12. Secondary structure prediction reveals that 76.5% of the amino acids from exon 1 are located in a loop. Potential phosphorylation, glycosylation, and myristoylation sites were revealed through motif searches. Housekeeping PBG-D contains coiled-coil segments known to be involved in dynamic rearrangements in the active site.

Registro:

Documento: Artículo
Título:Sequence and structure of the rat housekeeping PBG-D isoform
Autor:Cardalda, C.A.; Batlle, A.; Juknat, A.A.
Filiación:Ctro. Invest. Sobre Porfirinas y P., CONICET, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Palabras clave:complementary DNA; isoenzyme; porphobilinogen deaminase; amino acid sequence; animal tissue; article; enzyme active site; enzyme glycosylation; enzyme phosphorylation; enzyme structure; exon; housekeeping gene; male; myristylation; nonhuman; nucleotide sequence; priority journal; protein secondary structure; rat; sequence homology; Animalia; Rodentia
Año:1998
Volumen:249
Número:2
Página de inicio:438
Página de fin:443
DOI: http://dx.doi.org/10.1006/bbrc.1998.9021
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:porphobilinogen deaminase, 9036-47-9, 9074-91-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v249_n2_p438_Cardalda

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Citas:

---------- APA ----------
Cardalda, C.A., Batlle, A. & Juknat, A.A. (1998) . Sequence and structure of the rat housekeeping PBG-D isoform. Biochemical and Biophysical Research Communications, 249(2), 438-443.
http://dx.doi.org/10.1006/bbrc.1998.9021
---------- CHICAGO ----------
Cardalda, C.A., Batlle, A., Juknat, A.A. "Sequence and structure of the rat housekeeping PBG-D isoform" . Biochemical and Biophysical Research Communications 249, no. 2 (1998) : 438-443.
http://dx.doi.org/10.1006/bbrc.1998.9021
---------- MLA ----------
Cardalda, C.A., Batlle, A., Juknat, A.A. "Sequence and structure of the rat housekeeping PBG-D isoform" . Biochemical and Biophysical Research Communications, vol. 249, no. 2, 1998, pp. 438-443.
http://dx.doi.org/10.1006/bbrc.1998.9021
---------- VANCOUVER ----------
Cardalda, C.A., Batlle, A., Juknat, A.A. Sequence and structure of the rat housekeeping PBG-D isoform. Biochem. Biophys. Res. Commun. 1998;249(2):438-443.
http://dx.doi.org/10.1006/bbrc.1998.9021