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Abstract:

The effects of the cyclin-dependent kinase (CDK) inhibitors olomoucine and roscovitine on DNA synthesis were studied using short time incubation (30-90 minutes). Both purine analogues at concentrations from 1-100 μM decreased the DNA synthesis of rat brain cortex in a dose-dependent manner and the maximum effect occurred within 30 min of incubation. Staurosporine, another potent CDK inhibitor did not affect the DNA synthesis in the concentration range 1-250 nM. These results indicate that olomoucine and roscovitine block DNA synthesis by a mechanism independent of CDK inhibition. We propose that the cellullar effects of olomuocine and roscovitine on the cell cycle are at least in part due to this early inhibitory effect on DNA synthesis.

Registro:

Documento: Artículo
Título:Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine
Autor:Yakisich, J.S.; Sidén, A.; Vargas, V.I.; Eneroth, P.; Cruz, M.
Filiación:Applied Biochemistry, Clinical Research Center, Huddinge University Hospital, S-141 86, Huddinge, Sweden
Department of Neurology, Karolinska Institute, Huddinge University Hospital, S-141 86, Huddinge, Sweden
Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:cyclin dependent kinase; cyclin dependent kinase inhibitor; purine derivative; animal cell; animal tissue; article; brain cortex; cell cycle; DNA synthesis inhibition; nonhuman; priority journal; rat; time; Animalia
Año:1998
Volumen:243
Número:3
Página de inicio:674
Página de fin:677
DOI: http://dx.doi.org/10.1006/bbrc.1998.8157
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:cyclin dependent kinase, 150428-23-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p674_Yakisich

Referencias:

  • Vesely, J., Havlicek, L., Strnad, M., Blow, J.J., Donella-Deana, A., Pinna, L., Letham, D.S., Meijer, L., (1994) Eur. J. Biochem., 224, pp. 771-786
  • Meijer, L., Borgne, A., Mulner, O., Chong, J.P.J., Blow, J.J., Inagaki, N., Inagaki, M., Moulinoux, J-P., (1997) Eur. J. Biochem., 243, pp. 527-536
  • Glab, N., Labidi, B., Qin, L-X., Trehin, C., Bergounioux, C., Meijer, L., (1994) FEBS Lett., 353, pp. 207-211
  • Abraham, R.T., Acquarone, M., Andersen, A., Asensi, A., Belle, R., Berger, F., Bergounioux, C., Verlhac, M.H., (1995) Biol. Cell., 83, pp. 105-120
  • Meijer, L., (1996) Trends Cell Biol, 6, pp. 393-397
  • Alperin, D.M., Idoyaga-Vargas, V.P., Carminatti, H., (1986) J. Neurochem., 47, pp. 355-362
  • Labarca, C., Paigen, K., (1980) Anal. Biochem., 102, pp. 344-352
  • Nurse, P., Bisset, Y., (1981) Nature, 292, pp. 558-560
  • Gadbois, D.M., Hamaguchi, J.R., Swank, R.A., Bradbury, M.E., (1992) Biochem. Biophys. Res. Comm., 184, pp. 80-85
  • Veerana, S.K.T., Amin, N., Grant, P., Albers, R.W., Pant, H.C., (1996) Neurochem. Res., 21, pp. 629-636
  • Hall, L.L., Th'Ng, J.P.H., Guo, X.W., Teplitz, R.L., Bradbury, M.E., (1996) Cancer Res., 56, pp. 3551-3559
  • Yakisich, J.S., Radrizzani, M., Idoyaga Vargas, V., (1994) Neurosci. Lett., 180, pp. 17-20
  • Blow, J.J., Laskey, R.A., (1988) Nature, 332, pp. 546-548
  • Chong, J.P.J., Mahbubani, H.M., Khoo, C-Y., Blow, J.J., (1995) Nature, 375, pp. 418-421
  • Shields, R., (1977) Nature, 267, pp. 704-707
  • McAuley, A., Werb, Z., Mirkes, P.E., (1993) Development, 117, pp. 873-883
  • Weinert, T.A., Hartwell, L.H., (1988) Science, 241, pp. 317-322
  • Hartwell, L.H., Weinert, T.A., (1989) Science, 246, pp. 629-634
  • Hartwell, L.H., Kastan, M.B., (1994) Science, 266, pp. 1821-1828

Citas:

---------- APA ----------
Yakisich, J.S., Sidén, A., Vargas, V.I., Eneroth, P. & Cruz, M. (1998) . Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine. Biochemical and Biophysical Research Communications, 243(3), 674-677.
http://dx.doi.org/10.1006/bbrc.1998.8157
---------- CHICAGO ----------
Yakisich, J.S., Sidén, A., Vargas, V.I., Eneroth, P., Cruz, M. "Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine" . Biochemical and Biophysical Research Communications 243, no. 3 (1998) : 674-677.
http://dx.doi.org/10.1006/bbrc.1998.8157
---------- MLA ----------
Yakisich, J.S., Sidén, A., Vargas, V.I., Eneroth, P., Cruz, M. "Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine" . Biochemical and Biophysical Research Communications, vol. 243, no. 3, 1998, pp. 674-677.
http://dx.doi.org/10.1006/bbrc.1998.8157
---------- VANCOUVER ----------
Yakisich, J.S., Sidén, A., Vargas, V.I., Eneroth, P., Cruz, M. Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine. Biochem. Biophys. Res. Commun. 1998;243(3):674-677.
http://dx.doi.org/10.1006/bbrc.1998.8157