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Florin-Christensen, J.; D'Alessio, C.; Arighi, C.; Caramelo, J.; Florin-Christensen, M.; Delfino, J.M. "Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol" (1998) Biochemical and Biophysical Research Communications. 243(3):669-673
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Abstract:

Previous work indicated that diacylglycerol (DG) molecules translocate across the cytoplasm of mammalian cells, a process relevant to the signalling role of this lipid as protein kinase C activator. Here we investigated the possible mechanism underlying DG translocation. We examined the interaction between 1,2-di-[1-14C]oleoyl-sn-glycerol and rat liver cytosol (rlc) using assays based on Lipidex-1000 and on coelution on Sepharose CL 6B. We measured high DG binding activity and found that it resides in cytosolic proteins and not in cytosolic lipids. Chromatography of rlc proteins on Sepharose CL 6B showed profiles in which the activity measured by either method coincided. Further, we showed that the DG-rlc protein interaction results in the stabilization of DG in a micellar form, eluting in the void volume of Sepharose CL 6B. Such stabilized micelles are reminiscent of insect lipophorins and may represent a new, thus far unrecognized, mode of lipid transport within living cells.

Registro:

Documento: Artículo
Título:Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
Autor:Florin-Christensen, J.; D'Alessio, C.; Arighi, C.; Caramelo, J.; Florin-Christensen, M.; Delfino, J.M.
Filiación:Institute of Neuroscience (INEUCI), Ciudad Universitaria, Pabellón II, RA-1428, Buenos Aires, Argentina
Inst. Biochem. Biophys. (IQUIFIB), Junín 956, RA-1113, Buenos Aires, Argentina
Palabras clave:diacylglycerol; lipoprotein; protein kinase C activator; sepharose; animal cell; article; binding affinity; column chromatography; cytoplasm; intracellular transport; lipid transport; liver cytosol; micelle; nonhuman; priority journal; protein interaction; rat; Animalia; Insecta; Mammalia
Año:1998
Volumen:243
Número:3
Página de inicio:669
Página de fin:673
DOI: http://dx.doi.org/10.1006/bbrc.1998.8155
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:sepharose, 61970-08-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p669_FlorinChristensen

Referencias:

  • Nishizuka, Y., (1992) Science, 258, pp. 607-614
  • Florin-Christensen, J., Florin-Christensen, M., Meinardi, E., Calle, R., (1996) Biochem. J., 315, pp. 513-516
  • Dlugovz, A.A., Yuspa, S.H., (1993) J. Cell Biol., 120, pp. 217-225
  • Calle, R., Ganesan, S., Smallwood, J.I., Rasmussen, H., (1992) J. Biol. Chem., 267, pp. 18723-18727
  • Takuwa, Y., Kelley, G., Takuwa, N., Rasmussen, H., (1988) Mol. Cell. Endocrinol., 60, pp. 71-86
  • Gurr, M. Hartwood, J. L. 1991; Pagano, R.E., Sleight, R.G., (1985) Science, 229, pp. 1051-1057
  • Florin-Christensen, J., Florin-Christensen, M., Delfino, J.M., Stegmann, T., Rasmussen, H., (1992) J. Biol. Chem., 267, pp. 14783-14789
  • Florin-Christensen, J., Florin-Christensen, M., Delfino, J.M., Rasmussen, H., (1993) Biochem. J., 289, pp. 783-788
  • Mochly-Rosen, D., (1995) Science, 268, pp. 247-251
  • Bell, R.M., (1986) Cell, 45, pp. 631-632
  • Wirtz, K.W.A., (1982) Lipid-Protein Interactions, , New York: Wiley. p. 151-231
  • Bass, N.M., (1990) Mol. Cell. Biochem., 98, pp. 167-176
  • Clarke, S.D., Armstrong, M.K., (1989) FASEB J., 3, pp. 2480-2487
  • Banaszak, L., Winter, N., Xu, Z., Bernlohr, D.A., Cowan, S., Jones, T.A., (1994), 45, p. 89. , in 151, Academic Press, New York; Greenberg, A.S., Egan, J.J., Wek, S.A., Garty, N.B., Blanchette-Mackie, J., Londos, C., (1991) J. Biol. Chem., 266, pp. 11341-11346
  • Greenberg, A.S., Egan, J.J., Wek, S.A., Garty, N.B., Moos M.C., Jr., Londos, C., Kimmel, A.R., (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 12035-12039
  • Brasaemle, D.L., Barber, T., Kimmel, A.R., Londos, C., (1997) J. Biol. Chem., 272, pp. 9378-9387
  • Colley, C.M., Zwaal, R.F.A., Roelofsen, B., Van Deenen, L.L.M., (1973) Biochim. Biophys. Acta, 307, pp. 74-82
  • Dawson, R.M.C., Hennington, N.L., Irvine, R.F., (1983) Biochem. Biophys. Res. Commun., 117, pp. 196-201
  • Das, S., Rand, R.P., (1986) Biochemistry, 25, pp. 2882-2889
  • De Boeck, H., Zidovetski, R., (1989) Biochemistry, 28, pp. 7439-7446
  • Banschbach, M.W., Geison, R.L., Hokin-Neaverson, M., (1974) Biochem. Biophys. Res. Commun., 58, pp. 714-718
  • Hughes, B.P., (1984) Biochem. J., 222, pp. 535-540
  • Tardi, P.G., Man, R.Y.K., Choy, P.C., (1992) Biochem. J., 285, pp. 161-166
  • Bligh, E.G., Dyer, W.J., (1959) Can. J. Biochem. Physiol., 37, pp. 911-917
  • Glatz, J.F., Veerkamp, J.H., (1983) Anal. Biochem., 132, pp. 89-95
  • Lowry, O.H., Rosebrough, N.J., Far, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Dahlberg, E., Snochowski, M., Gustaffson, J.-A., (1980) Anal. Biochem., 106, pp. 380-388
  • Wang, J., Liu, H., Sykes, B.D., Ryan, R.O., (1995) Biochemistry, 34, pp. 6755-6761
  • Ryan, R.O., (1996) Biochem. Cell Biol., 74, pp. 155-164
  • Soulages, J.L., Van Antwerpen, R., Wells, M.A., (1996) Biochemistry, 35, pp. 5191-5198

Citas:

---------- APA ----------
Florin-Christensen, J., D'Alessio, C., Arighi, C., Caramelo, J., Florin-Christensen, M. & Delfino, J.M. (1998) . Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol. Biochemical and Biophysical Research Communications, 243(3), 669-673.
http://dx.doi.org/10.1006/bbrc.1998.8155
---------- CHICAGO ----------
Florin-Christensen, J., D'Alessio, C., Arighi, C., Caramelo, J., Florin-Christensen, M., Delfino, J.M. "Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol" . Biochemical and Biophysical Research Communications 243, no. 3 (1998) : 669-673.
http://dx.doi.org/10.1006/bbrc.1998.8155
---------- MLA ----------
Florin-Christensen, J., D'Alessio, C., Arighi, C., Caramelo, J., Florin-Christensen, M., Delfino, J.M. "Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol" . Biochemical and Biophysical Research Communications, vol. 243, no. 3, 1998, pp. 669-673.
http://dx.doi.org/10.1006/bbrc.1998.8155
---------- VANCOUVER ----------
Florin-Christensen, J., D'Alessio, C., Arighi, C., Caramelo, J., Florin-Christensen, M., Delfino, J.M. Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol. Biochem. Biophys. Res. Commun. 1998;243(3):669-673.
http://dx.doi.org/10.1006/bbrc.1998.8155