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Abstract:

The catalytic properties of ornithine decarboxylase (ODC) from Leishmania mexicana as well as the interaction with its cofactor pyridoxal 5'-phosphate (PLP) and the irreversible inhibitor α-difluoromethylornithine (DFMO) have been studied using partially purified preparations of the enzyme obtained from parasite promastigotes. Leishmania extracts prepared in the presence of saturating concentrations of PLP yielded an enzyme considerably more resistant to heat inactivation and with a three-fold higher activity than the ODC obtained without the addition of cofactor. The complete removal of PLP by treatment with hydroxylamine yielded the apoenzyme which shows an absolute requirement for PLP to recover its enzymatic activity. The K(m) values for L-ornithine and PLP were 0.7 mM and 25 μM, respectively, while K(i) for DFMO was 0.2 mM. The restoration of ODC activity from apoenzyme and cofactor seems to involve time and temperature-dependent activation processes. L. mexicana ODC has an apparent molecular mass of 240 ± 20 kDa. © 1995 Academic Press, Inc.

Registro:

Documento: Artículo
Título:Ornithine decarboxylase from Leishmania mexicana promastigotes: Interaction with pyridoxal 5'-phosphate and α-difluoromethylornithine
Autor:Sénchez, C.P.; Sidrauski, C.; Freire, S.M.; González, N.S.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioqufmicas "Fundación Campomar", Facultad de Ciencias Exactas y Naturales, University of Buenos Aires and CONICET, A. Machado 151, 1405 Buenos Aires, Argentina
Universidade Federal de Barnía, Brazil
Año:1995
Volumen:212
Número:2
Página de inicio:396
Página de fin:403
DOI: http://dx.doi.org/10.1006/bbrc.1995.1983
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:BIOCHEM. BIOPHYS. RES. COMMUN.
ISSN:0006291X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v212_n2_p396_Senchez

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Citas:

---------- APA ----------
Sénchez, C.P., Sidrauski, C., Freire, S.M., González, N.S. & Algranati, I.D. (1995) . Ornithine decarboxylase from Leishmania mexicana promastigotes: Interaction with pyridoxal 5'-phosphate and α-difluoromethylornithine. Biochemical and Biophysical Research Communications, 212(2), 396-403.
http://dx.doi.org/10.1006/bbrc.1995.1983
---------- CHICAGO ----------
Sénchez, C.P., Sidrauski, C., Freire, S.M., González, N.S., Algranati, I.D. "Ornithine decarboxylase from Leishmania mexicana promastigotes: Interaction with pyridoxal 5'-phosphate and α-difluoromethylornithine" . Biochemical and Biophysical Research Communications 212, no. 2 (1995) : 396-403.
http://dx.doi.org/10.1006/bbrc.1995.1983
---------- MLA ----------
Sénchez, C.P., Sidrauski, C., Freire, S.M., González, N.S., Algranati, I.D. "Ornithine decarboxylase from Leishmania mexicana promastigotes: Interaction with pyridoxal 5'-phosphate and α-difluoromethylornithine" . Biochemical and Biophysical Research Communications, vol. 212, no. 2, 1995, pp. 396-403.
http://dx.doi.org/10.1006/bbrc.1995.1983
---------- VANCOUVER ----------
Sénchez, C.P., Sidrauski, C., Freire, S.M., González, N.S., Algranati, I.D. Ornithine decarboxylase from Leishmania mexicana promastigotes: Interaction with pyridoxal 5'-phosphate and α-difluoromethylornithine. BIOCHEM. BIOPHYS. RES. COMMUN. 1995;212(2):396-403.
http://dx.doi.org/10.1006/bbrc.1995.1983